Divergent macrophage responses to Mycobacterium bovis among naturally exposed uninfected and infected cattle

Mycobacterium bovis, the causative agent of bovine tuberculosis (TB), is a successful pathogen that remains an important global threat to livestock. Cattle naturally exposed to M. bovis normally become reactive to the M. bovis‐purified protein derivative (tuberculin) skin test; however, some individ...

Full description

Saved in:

Bibliographic Details
Published in:	Immunology and cell biology Vol. 95; no. 5; pp. 436 - 442
Main Authors:	Alcaraz‐López, Omar A, García‐Gil, Cindy, Morales‐Martínez, Claudia, López‐Rincón, Gonzalo, Estrada‐Chávez, Ciro, Gutiérrez‐Pabello, José A, Esquivel‐Solís, Hugo
Format:	Journal Article
Language:	English
Published:	United States Nature Publishing Group 01-05-2017 Blackwell Science Ltd
Subjects:	Animals Cattle Cell Survival Chemokine CCL2 - genetics Chemokine CCL2 - metabolism Female Gene Expression Regulation Interleukin-10 - genetics Interleukin-10 - metabolism Interleukin-12 - genetics Interleukin-12 - metabolism Macrophages - immunology Macrophages - microbiology Mycobacterium bovis Mycobacterium bovis - physiology Nitric Oxide - metabolism Nitric Oxide Synthase Type II - genetics Nitric Oxide Synthase Type II - metabolism Phagocytosis Superoxides - metabolism Tuberculosis, Bovine - genetics Tuberculosis, Bovine - immunology Tuberculosis, Bovine - microbiology Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Mycobacterium bovis, the causative agent of bovine tuberculosis (TB), is a successful pathogen that remains an important global threat to livestock. Cattle naturally exposed to M. bovis normally become reactive to the M. bovis‐purified protein derivative (tuberculin) skin test; however, some individuals remain negative, suggesting that they may be resistant to infection. To better understand host innate resistance to infection, 26 cattle from herds with a long history of high TB prevalence were included in this study. We investigated the bactericidal activity, the production of reactive oxygen and nitrogen species and the TB‐related gene expression profile after in vitro M. bovis challenge of monocyte‐derived macrophages from cattle with TB (n=17) and from non‐infected, exposed cattle (in‐contacts, n=9). The disease status was established based on the tuberculin skin test and blood interferon‐gamma test responses, the presence of visible lesions at inspection on abattoirs and the histopathology and culture of M. bovis. Although macrophages from TB‐infected cattle enabled M. bovis replication, macrophages from healthy, exposed cattle had twofold lower bacterial loads, overproduced nitric oxide and had lower interleukin (IL)‐10 gene expression (P⩽0.05). Higher mRNA expression levels of inducible nitric oxide synthase, C‐C motif chemokine ligand 2 and IL‐12 were observed in macrophages from all in‐contact cattle than in macrophages from their TB‐infected counterparts, which expressed more tumour necrosis factor‐α; however, the differences were not statistically significant owing to individual variation. These results confirm that macrophage bactericidal responses have a crucial role in innate resistance to M. bovis infection in cattle.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0818-9641 1440-1711
DOI:	10.1038/icb.2016.114