Search Results - "Garber, Maria B"
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Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1
Published in Cell reports (Cambridge) (18-07-2017)“…The repertoire of the density-regulated protein (DENR) and the malignant T cell-amplified sequence 1 (MCT-1/MCTS1) oncoprotein was recently expanded to include…”
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Protein L5 is crucial for in vivo assembly of the bacterial 50S ribosomal subunit central protuberance
Published in Nucleic acids research (01-10-2012)“…In the present work, ribosomes assembled in bacterial cells in the absence of essential ribosomal protein L5 were obtained. After arresting L5 synthesis,…”
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Structure and Function of Archaeal Translation Initiation Factor 2 Fragments Containing Cys2‑Cys2 Motifs
Published in Biochemistry (Moscow) (01-08-2021)“…The heterotrimeric (αβγ) translation initiation factor 2 of archaea and eukaryotes (a/eIF2) supplies the P-site of the ribosome with the initiation tRNA. Its…”
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4
Four translation initiation pathways employed by the leaderless mRNA in eukaryotes
Published in Scientific reports (28-11-2016)“…mRNAs lacking 5′ untranslated regions (leaderless mRNAs) are molecular relics of an ancient translation initiation pathway. Nevertheless, they still represent…”
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Hydration shells of molecules in molecular association: A mechanism for biomolecular recognition
Published in Journal of theoretical biology (21-05-2012)“…It has become clear that water should not be treated as an inert environment, but rather as an integral and active component of molecules. Here, we consider…”
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Archaeal Translation Initiation Factor aIF2 Can Substitute for Eukaryotic eIF2 in Ribosomal Scanning during Mammalian 48S Complex Formation
Published in Journal of molecular biology (14-10-2011)“…Heterotrimeric translation initiation factor (IF) a/eIF2 ( archaeal/ eukaryotic IF 2) is present in both Eukarya and Archaea. Despite strong structural…”
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Studying the Properties of Domain I of the Ribosomal Protein L1: Incorporation into Ribosome and Regulation of the L1 Operon Expression
Published in Protein Journal (01-04-2015)“…L1 is a conserved protein of the large ribosomal subunit. This protein binds strongly to the specific region of the high molecular weight rRNA of the large…”
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30S ribosomal subunits can be assembled in vivo without primary binding ribosomal protein S15
Published in RNA (Cambridge) (01-07-2006)“…Assembly of 30S ribosomal subunits from Escherichia coli has been dissected in detail using an in vitro system. Such studies have allowed characterization of…”
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Importance of the 5 S rRNA-binding Ribosomal Proteins for Cell Viability and Translation in Escherichia coli
Published in Journal of molecular biology (02-03-2007)“…A specific complex of 5 S rRNA and several ribosomal proteins is an integral part of ribosomes in all living organisms. Here we studied the importance of…”
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10
Analysis of Recognition of Transfer-messenger RNA by the Ribosomal Decoding Center
Published in Journal of molecular biology (18-02-2005)“…Bacterial ribosomes stalled on defective mRNAs are rescued by tmRNA that functions as both tRNA and mRNA. The first ribosomal elongation cycle on tmRNA where…”
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Ribosomal Elongation Cycle: Energetic, Kinetic and Stereochemical Aspects
Published in Journal of molecular biology (19-08-2005)“…As a preface to an analysis of the ribosomal elongation cycle, we examine the energetics of macromolecular structural transformations. We show that the kinetic…”
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The crucial role of conserved intermolecular H-bonds inaccessible to the solvent in formation and stabilization of the TL5.5 SrRNA complex
Published in The Journal of biological chemistry (22-04-2005)“…Analysis of the structures of two complexes of 5 S rRNA with homologous ribosomal proteins, Escherichia coli L25 and Thermus thermophilus TL5, revealed that…”
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13
NMR structure of the ribosomal protein L23 from Thermus thermophilus
Published in Journal of biomolecular NMR (01-06-2003)“…The ribosomal protein L23 is a component of the large ribosomal subunit in which it is located close to the peptide exit tunnel. In this position L23 plays a…”
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The Crucial Role of Conserved Intermolecular H-bonds Inaccessible to the Solvent in Formation and Stabilization of the TL5·5 SrRNA Complex
Published in The Journal of biological chemistry (01-04-2005)“…Analysis of the structures of two complexes of 5 S rRNA with homologous ribosomal proteins, Escherichia coli L25 and Thermus thermophilus TL5, revealed that…”
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15
The solution structure of ribosomal protein L18 from Thermus thermophilus reveals a conserved RNA-binding fold
Published in Biochemical journal (01-05-2002)“…We have determined the solution structure of ribosomal protein L18 from Thermus thermophilus. L18 is a 12.5 kDa protein of the large subunit of the ribosome…”
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Crystallization of Hfq protein: a bacterial gene-expression regulator
Published in Acta crystallographica. Section D, Biological crystallography. (01-06-2003)“…Hfq protein from Escherichia coli (EcoHfq) has been overproduced in E. coli, purified to homogeneity and crystallized using the hanging‐drop vapour‐diffusion…”
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Structure and Function of Archaeal Translation Initiation Factor 2 Fragments Containing Cys2âCys2 Motifs
Published in Biochemistry (Moscow) (01-08-2021)“…The heterotrimeric ([alpha][beta][gamma]) translation initiation factor 2 of archaea and eukaryotes (a/eIF2) supplies the P-site of the ribosome with the…”
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Another piece of the ribosome: solution structure of S16 and its location in the 30S subunit
Published in Structure (London) (15-08-2000)“…Background: X-ray crystallography has recently yielded much-improved electron-density maps of the bacterial ribosome and its two subunits and many structural…”
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Preliminary investigation of the three-dimensional structure of Salmonella typhimurium uridine phosphorylase in the crystalline state
Published in Acta crystallographica. Section F, Structural biology and crystallization communications (01-04-2005)“…Uridine phosphorylase (UPh) catalyzes the phosphorolytic cleavage of the C—N glycosidic bond of uridine to ribose 1‐phosphate and uracil in the…”
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20
Proteolytic fragmentation of polypeptide release factor 1 of Thermus thermophilus and crystallization of the stable fragments
Published in Biochimie (01-08-2000)“…Polypeptide release factor one from Thermus thermophilus, ttRF1, was purified and subjected to crystallization. Thin crystalline needles were obtained but…”
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