Droplet Digital PCR Quantification of Selected Intracellular and Extracellular microRNAs Reveals Changes in Their Expression Pattern during Porcine In Vitro Adipogenesis

Droplet Digital PCR Quantification of Selected Intracellular and Extracellular microRNAs Reveals Changes in Their Expression Pattern during Porcine In Vitro Adipogenesis

Extracellular miRNAs have attracted considerable interest because of their role in intercellular communication, as well as because of their potential use as diagnostic and prognostic biomarkers for many diseases. It has been shown that miRNAs secreted by adipose tissue can contribute to the pathophy...

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Bibliographic Details
Published in:Genes Vol. 14; no. 3; p. 683
Main Authors: Bilinska, Adrianna, Pszczola, Marcin, Stachowiak, Monika, Stachecka, Joanna, Garbacz, Franciszek, Aksoy, Mehmet Onur, Szczerbal, Izabela
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 09-03-2023
MDPI
Subjects:
Adipocytes
Adipogenesis
Adipogenesis - genetics
Adipose tissue
Adipose Tissue - metabolism
Adipose tissues
Animals
Antibiotics
Body fat
Cell culture
Cell differentiation
Cell Differentiation - genetics
Communication
Genes
Inflammation
Intracellular
Mesenchymal stem cells
MicroRNA
MicroRNAs
MicroRNAs - metabolism
miRNA
Polymerase Chain Reaction
Proteins
Stem cells
Swine
United States > US
Germany
ddPCR
ECmiRNAs
adipocytes
mesenchymal stem cells
pig
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Summary:Extracellular miRNAs have attracted considerable interest because of their role in intercellular communication, as well as because of their potential use as diagnostic and prognostic biomarkers for many diseases. It has been shown that miRNAs secreted by adipose tissue can contribute to the pathophysiology of obesity. Detailed knowledge of the expression of intracellular and extracellular microRNAs in adipocytes is thus urgently required. The system of in vitro differentiation of mesenchymal stem cells (MSCs) into adipocytes offers a good model for such an analysis. The aim of this study was to quantify eight intracellular and extracellular miRNAs (miR-21a, miR-26b, miR-30a, miR-92a, miR-146a, miR-148a, miR-199, and miR-383a) during porcine in vitro adipogenesis using droplet digital PCR (ddPCR), a highly sensitive method. It was found that only some miRNAs associated with the inflammatory process (miR-21a, miR-92a) were highly expressed in differentiated adipocytes and were also secreted by cells. All miRNAs associated with adipocyte differentiation were highly abundant in both the studied cells and in the cell culture medium. Those miRNAs showed a characteristic expression profile with upregulation during differentiation.
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ISSN:2073-4425
2073-4425
DOI:10.3390/genes14030683