An S-locus Receptor Protein Kinase from Peach is Expressed Predominantly in Fruit and Responds Differently to Various External Stresses

A receptor-like protein kinase (RLK) isolated from December peach bark (Ppsrlk1) was previously shown to have considerable homology to the S -locus class of RLKs associated with reproductive self-incompatibility. Unlike those RLKs, transcripts arising from Ppsrlk1 were not detectable in standard RNA...

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Bibliographic Details
Published in:	HortScience Vol. 33; no. 3; p. 532
Main Authors:	Bassett, C.L, Nickerson, M, Artlip, T.A, Wisniewski, M, Gaouth, A. El, Wilson, C
Format:	Journal Article
Language:	English
Published:	01-06-1998
Online Access:	Get full text
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Summary:	A receptor-like protein kinase (RLK) isolated from December peach bark (Ppsrlk1) was previously shown to have considerable homology to the S -locus class of RLKs associated with reproductive self-incompatibility. Unlike those RLKs, transcripts arising from Ppsrlk1 were not detectable in standard RNA blots. To determine the pattern of expression of this gene, RNAs from various peach tissues, some of which had been subjected to different stress treatments, were surveyed using Reverse Transcript-PCR (RT-PCR). Based on these results, select RNAs were further quantitated by Competitive PCR (C-PCR). RT-PCR of RNAs from seasonal peach bark revealed little change in the relative abundance of Ppsrlk1, although a slight decrease was noted in the RNAs from October and December bark. Expression of Ppsrlk1 in developing peach fruit peaked at stage II and declined thereafter from stage III and in the later stages of ripening. Ripe peach fruit subjected to 10 min UV treatment or wounding followed by application of a yeast biocontrol agent showed a further decline in Ppsrlk1 expression; C-PCR analysis of the wounded fruit indicated a five-fold difference in expression between wounded water controls and wounded fruit treated with the biocontrol agen 48 h after treatment. In bark and xylem tissues under water deficit conditions expression of Ppsrlk1 declined. In contrast, levels of Ppsrlk1 in leaves and roots increased. C-PCR of the root samples revealed a 50-fold increase in the transcript in response to water deficit.
ISSN:	0018-5345 2327-9834
DOI:	10.21273/HORTSCI.33.3.532b