Search Results - "Gao, Xinliu"
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Targeted Host Cell Protein Quantification by LC–MRM Enables Biologics Processing and Product Characterization
Published in Analytical chemistry (Washington) (07-01-2020)“…Multiple reaction monitoring (MRM) is a liquid chromatography–mass spectrometry (LC–MS) based quantification platform with high sensitivity, specificity, and…”
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Determination of ultra-trace metal-protein interactions in co-formulated monoclonal antibody drug product by SEC-ICP-MS
Published in mAbs (2023)“…Transition metals can be introduced in therapeutic protein drugs at various steps of the manufacturing process (e.g. manufacturing raw materials, formulation,…”
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Enzymatic activity of the alternative complex III as a menaquinol:auracyanin oxidoreductase in the electron transfer chain of Chloroflexus aurantiacus
Published in FEBS letters (06-10-2009)“…The surprising lack of the cytochrome bc 1 complex in the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus suggests that a functional…”
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Structural Analysis of Alternative Complex III in the Photosynthetic Electron Transfer Chain of Chloroflexus aurantiacus
Published in Biochemistry (Easton) (10-08-2010)“…The green photosynthetic bacterium Chloroflexus aurantiacus, which belongs to the phylum of filamentous anoxygenic phototrophs, does not contain a cytochrome…”
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Extended characterization of unpaired cysteines in an IgG1 monoclonal antibody by LC-MS analysis
Published in Analytical biochemistry (01-06-2021)“…The development of comprehensive methods to characterize unpaired cysteines in monoclonal antibodies (mAbs) is very important for understanding structural…”
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Precise O‑Glycosylation Site Localization of CD24Fc by LC-MS Workflows
Published in Analytical chemistry (Washington) (14-06-2022)“…CD24Fc is a homodimeric recombinant Fc-fusion protein comprised of human CD24 connected to immunoglobulin G1 (IgG1) Fc fragment. CD24 is heavily glycosylated,…”
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LC–MS Approach to Decipher a Light Chain Chromatographic Peak Splitting of a Monoclonal Antibody
Published in Pharmaceutical research (01-12-2023)“…Purpose Monoclonal antibodies (mAbs), like other protein therapeutics, are prone to various forms of degradation, some of which are difficult to distinguish…”
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Temporal Changes in Milk Proteomes Reveal Developing Milk Functions
Published in Journal of proteome research (06-07-2012)“…Human milk proteins provide essential nutrition for growth and development, and support a number of vital developmental processes in the neonate. A complete…”
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Discovery and Control of Succinimide Formation and Accumulation at Aspartic Acid Residues in The Complementarity-Determining Region of a Therapeutic Monoclonal Antibody
Published in Pharmaceutical research (01-06-2023)“…Purpose Succinimide formation and isomerization alter the chemical and physical properties of aspartic acid residues in a protein. Modification of aspartic…”
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Identification and characterization of a residual host cell protein hexosaminidase B associated with N‐glycan degradation during the stability study of a therapeutic recombinant monoclonal antibody product
Published in Biotechnology progress (01-05-2021)“…Host cell proteins (HCPs) are process‐related impurities derived from host organisms, which need to be controlled to ensure adequate product quality and…”
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Volumetric absorptive microsampling (VAMS®) in therapeutic protein quantification by LC-MS/MS: Investigation of anticoagulant impact on assay performance and recommendations for best practices in method development
Published in Journal of pharmaceutical and biomedical analysis (20-03-2021)“…•We describe the method development, validation, and a proof-of-concept non-human primate study of a LC–MS method for VAMS utilized to obtain PK data for a…”
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Functional analysis and expression of the mono-heme containing cytochrome c subunit of alternative complex III in Chloroflexus aurantiacus
Published in Archives of biochemistry and biophysics (15-07-2013)“…•ActE subunit of Alternative Complex III (ACIII) cloned and expressed.•ACIII treated with chaotropic agents to remove ActE.•ActE reconstituted to depleted…”
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Expression and characterization of the diheme cytochrome c subunit of the cytochrome bc complex in Heliobacterium modesticaldum
Published in Archives of biochemistry and biophysics (15-01-2012)“…► Diheme cytochrome c from heliobacterial cytochrome bc complex is expressed in Escherichia coli and purified. ► Mass spectra confirm the correct expression of…”
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14
Semi‐quantitative analysis of milk proteomes reveals new evolving activities in carbohydrate metabolism in breastfeeding women
Published in The FASEB journal (01-04-2012)“…Abstract only Human milk proteins are thought to support a number of fundamental biological processes in the neonate. Among these processes may be carbohydrate…”
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Proteomics reveals new evolving roles in defense and development from colostrum to mature milk
Published in The FASEB journal (01-04-2012)“…Abstract only Human milk proteins provide nutrition and support a number of vital biological processes in nursing infants. In‐depth understanding of the…”
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Quantitative analysis of factor P (Properdin) in monkey serum using immunoaffinity capturing in combination with LC-MS/MS
Published in Bioanalysis (01-01-2016)“…Factor P (Properdin), an endogenous glycoprotein, plays a key role in innate immune defense. Its quantification is important for understanding the…”
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Structural and functional study of Alternative Complex III in the photosynthetic electron transfer chain of Chloroflexus aurantiacus
Published 01-01-2011“…This dissertation focuses on the structural and functional study of a newly found electron transfer protein complex in Chloroflexus aurantiacus. The lack of…”
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Dissertation -
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Antitumor active monofunctional platinum(II) complexes: Synthesis, structural characterization and reactivity towards biomolecules
Published in Inorganic chemistry communications (01-07-2006)“…The platinum(II) complexes of l-valine- N-8-quinolylamide and l-leucine- N-8-quinolylamide were shown to bind to DNA with high affinity and demonstrated…”
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Structural and functional study of Alternative Complex III in the photosynthetic electron transfer chain of Chloroflexus aurantiacus
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Dissertation -
20
Determination of ultra-trace metal-protein interactions in co-formulated monoclonal antibody drug product by SEC-ICP-MS
Published in mAbs (31-12-2023)“…Transition metals can be introduced in therapeutic protein drugs at various steps of the manufacturing process (e.g. manufacturing raw materials, formulation,…”
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