Evaluation of the swab sampling method to recover viruses from fomites

Evaluation of the swab sampling method to recover viruses from fomites

•We tested the swab sampling method in surface samples.•Recovery efficiency for MNV-1 was significantly higher compared to PP7.•MNV-1 demonstrate to be a potential ICP for swab sampling method. The monitoring of virus contamination on fomites, especially at hospitals has been used for a more effecti...

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Bibliographic Details
Published in:Journal of virological methods Vol. 217; pp. 24 - 27
Main Authors: Ganime, A.C., Leite, J.P.G., de Abreu Corrêa, A., Melgaço, F.G., Carvalho-Costa, F.A., Miagostovich, M.P.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-06-2015
Subjects:
Bacteriophage PP7
Bacteriophages - isolation & purification
Fomites
Fomites - virology
Internal control process
MNV-1
Norovirus
Norovirus - isolation & purification
Real-Time Polymerase Chain Reaction
Reference Standards
Swab
Virology - methods
Virology - standards
MNV-1
Fomites
Swab
Bacteriophage PP7
Internal control process
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Summary:•We tested the swab sampling method in surface samples.•Recovery efficiency for MNV-1 was significantly higher compared to PP7.•MNV-1 demonstrate to be a potential ICP for swab sampling method. The monitoring of virus contamination on fomites, especially at hospitals has been used for a more effective evaluation of the microbiological quality of surfaces. Swab sampling is the method used currently, although the use of an internal control process (ICP) has not yet been assessed. The aim of this study is to determine the recovery rate of murine norovirus 1 (MNV-1) and bacteriophage PP7 on different surfaces in order to assess their potential use as an ICP. For this purpose both viruses were spiked experimentally both on porous and non-porous formic as well as on rubberized surfaces. Quantitative PCR (qPCR) showed a variable efficiency with a percentage recovery ranging from 0.6 to 77% according to viruses and surfaces. A global analysis suggested that MNV-1 could be used as a potential ICP for the swab sampling method.
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ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2015.02.009