Synthesis and characterization of new 1,3,4-thiadiazole derivatives: study of their antibacterial activity and CT-DNA binding

1,3,4-Thiadiazole molecules ( 1-4 ) were synthesized by the reaction of phenylthiosemicarbazide and methoxy cinnamic acid molecules in the presence of phosphorus oxychloride, and characterized with UV, FT-IR, 13 C-NMR, and 1 H-NMR methods. DFT calculations (b3lyp/6-311++G(d,p)) were performed to inv...

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Bibliographic Details
Published in:	RSC advances Vol. 12; no. 46; pp. 29627 - 29639
Main Authors:	Sayiner, Hakan S, Yilmazer, Mehmet I, Abdelsalam, Aisha. T, Ganim, Mohamed A, Baloglu, Cengiz, Altunoglu, Yasemin Celik, Gür, Mahmut, Saracoglu, Murat, Attia, Mohamed S, Mahmoud, Safwat A, Mohamed, Ekram H, Boukherroub, Rabah, Al-Shaalan, Nora Hamad, Alharthi, Sarah, Kandemirli, Fatma, Amin, Mohammed A
Format:	Journal Article
Language:	English
Published:	Cambridge Royal Society of Chemistry 17-10-2022 The Royal Society of Chemistry
Subjects:	Bacteria Chemical synthesis Chemistry Cinnamic acid Coliforms E coli Engineering Sciences Kinases Klebsiella NMR Nuclear magnetic resonance Physiochemistry Pseudomonas aeruginosa Thiadiazoles
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Summary:	1,3,4-Thiadiazole molecules ( 1-4 ) were synthesized by the reaction of phenylthiosemicarbazide and methoxy cinnamic acid molecules in the presence of phosphorus oxychloride, and characterized with UV, FT-IR, 13 C-NMR, and 1 H-NMR methods. DFT calculations (b3lyp/6-311++G(d,p)) were performed to investigate the structures' geometry and physiochemical properties. Their antibacterial activity was screened for various bacteria strains such as Enterobacter aerogenes , Escherichia coli ATCC 13048, Salmonella kentucky , Pseudomonas aeruginosa , Klebsiella pneumoniae , Proteus and Gram positive such as Staphylococcus aureus ATCC 25923, Listeria monocytogenes ATCC 7644, Enterococcus faecium , Enterococcus durans , Staphylococcus aureus ATCC, Serratia marcescens , Staphylococcus hominis , Staphylococcus epidermidis , alfa Streptococcus haemolyticus , Enterococcus faecium and found to have an inhibitory effect on Klebsiella pneumoniae and Staphylococcus hominis , while molecules 1 , 3 and 4 had an inhibitory effect on Staphylococcus epidermidis and alpha Streptococcus haemolyticus . The experimental results were supported by the docking study using the Kinase ThiM from Klebsiella pneumoniae . All the investigated compounds showed an inhibitory effect for the Staphylococcus epidermidis protein. In addition, the mechanism of the 1-4 molecule interaction with calf thymus-DNA (CT-DNA) was investigated by UV-vis spectroscopic methods. 1,3,4-Thiadiazole molecules showed an inhibitory effect for the Staphylococcus epidermidis protein.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2046-2069 2046-2069
DOI:	10.1039/d2ra02435g