Exposure to Carbon Nanotube Material: Assessment of Nanotube Cytotoxicity using Human Keratinocyte Cells

Carbon nanotubes are new members of carbon allotropes similar to fullerenes and graphite. Because of their unique electrical, mechanical, and thermal properties, carbon nanotubes are important for novel applications in the electronics, aerospace, and computer industries. Exposure to graphite and car...

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Bibliographic Details
Published in:	Journal of Toxicology and Environmental Health, Part A Vol. 66; no. 20; pp. 1909 - 1926
Main Authors:	Shvedova, Anna, Castranova, Vincent, Kisin, Elena, Schwegler-Berry, Diane, Murray, Ashley, Gandelsman, Vadim, Maynard, Andrew, Baron, Paul
Format:	Journal Article
Language:	English
Published:	Johnson Space Center Informa UK Ltd 24-10-2003 Taylor and Francis
Subjects:	Biological and medical sciences Cells, Cultured Chemical and industrial products toxicology. Toxic occupational diseases Free Radicals - metabolism Humans Keratinocytes - drug effects Life Sciences (General) Lipid Peroxidation - drug effects Medical sciences Metals and various inorganic compounds Microscopy, Electron, Scanning Nanotubes, Carbon - adverse effects Oxidative Stress - drug effects Toxicology Human Oxidative stress Skin disease Cell line Toxicity Carbon nanotubes Cytotoxicity Keratinocyte Skin Mechanism of action In vitro
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Summary:	Carbon nanotubes are new members of carbon allotropes similar to fullerenes and graphite. Because of their unique electrical, mechanical, and thermal properties, carbon nanotubes are important for novel applications in the electronics, aerospace, and computer industries. Exposure to graphite and carbon materials has been associated with increased incidence of skin diseases, such as carbon fiber dermatitis, hyperkeratosis, and naevi. We investigated adverse effects of single-wall carbon nanotubes (SWCNT) using a cell culture of immortalized human epidermal keratinocytes (HaCaT). After 18 h of exposure of HaCaT to SWCNT, oxidative stress and cellular toxicity were indicated by formation of free radicals, accumulation of peroxidative products, antioxidant depletion, and loss of cell viability. Exposure to SWCNT also resulted in ultrastructural and morphological changes in cultured skin cells. These data indicate that dermal exposure to unrefined SWCNT may lead to dermal toxicity due to accelerated oxidative stress in the skin of exposed workers.
Bibliography:	Johnson Space Center JSC-CN-8128 JSC ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1528-7394 1087-2620 2381-3504
DOI:	10.1080/713853956