Purification, Characterization and Degradation Performance of a Novel Dextranase from Penicillium cyclopium CICC-4022

A novel dextranase was purified from CICC-4022 by ammonium sulfate fractional precipitation and gel filtration chromatography. The effects of temperature, pH and some metal ions and chemicals on dextranase activity were investigated. Subsequently, the dextranase was used to produce dextran with spec...

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Bibliographic Details
Published in:	International journal of molecular sciences Vol. 20; no. 6; p. 1360
Main Authors:	Huang, Ruijie, Zhong, Lei, Xie, Fengwei, Wei, Liming, Gan, Lanfang, Wang, Xuejiao, Liao, Anping
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 18-03-2019 MDPI
Subjects:	Acid resistance Bacteria Cellulose Chitin Chitosan Chromatography Cyclodextrins degradation Dextran Dextranase Dextrans Enzymes Fungi Glucose Mass distribution molecular mass Molecular weight Penicillium Penicillium cyclopium Purification Sucrose β-Cyclodextrin dextran degradation Penicillium cyclopium dextranase molecular mass purification
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Summary:	A novel dextranase was purified from CICC-4022 by ammonium sulfate fractional precipitation and gel filtration chromatography. The effects of temperature, pH and some metal ions and chemicals on dextranase activity were investigated. Subsequently, the dextranase was used to produce dextran with specific molecular mass. Weight-average molecular mass ( ) and the ratio of weight-average molecular mass/number-average molecular mass, or polydispersity index ( / ), of dextran were measured by multiple-angle laser light scattering (MALS) combined with gel permeation chromatography (GPC). The dextranase was purified to 16.09-fold concentration; the recovery rate was 29.17%; and the specific activity reached 350.29 U/mg. of the dextranase was 66 kDa, which is similar to dextranase obtained from other species reported previously. The highest activity was observed at 55 °C and a pH of 5.0. This dextranase was identified as an endodextranase, which specifically degraded the α-1,6 glucosidic bonds of dextran. According to metal ion dependency tests, Li⁺, Na⁺ and Fe were observed to effectively improve the enzymatic activity. In particular, Li⁺ could improve the activity to 116.28%. Furthermore, the dextranase was efficient at degrading dextran and the degradation rate can be well controlled by the dextranase activity, substrate concentration and reaction time. Thus, our results demonstrate the high potential of this dextranase from CICC-4022 as an efficient enzyme to produce specific clinical dextrans.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms20061360