Search Results - "Gamer, Martin"

Unraveling what makes a monoclonal antibody difficult‐to‐express: From intracellular accumulation to incomplete folding and degradation via ERAD by Mathias, Sven, Wippermann, Anna, Raab, Nadja, Zeh, Nikolas, Handrick, René, Gorr, Ingo, Schulz, Patrick, Fischer, Simon, Gamer, Martin, Otte, Kerstin

“…Although most therapeutic monoclonal antibodies (mAbs) can routinely be produced in the multigram per litre range, some mAb candidates turn out to be…”
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Polysaccharide Synthesis of the Levansucrase SacB from Bacillus megaterium Is Controlled by Distinct Surface Motifs by Strube, Christian P., Homann, Arne, Gamer, Martin, Jahn, Dieter, Seibel, Jürgen, Heinz, Dirk W.

“…Despite the widespread biological function of carbohydrates, the polysaccharide synthesis mechanisms of glycosyltransferases remain largely unexplored…”
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High-Yield Intra- and Extracellular Protein Production Using Bacillus megaterium by Stammen, Simon, Müller, Britta Katrin, Korneli, Claudia, Biedendieck, Rebekka, Gamer, Martin, Franco-Lara, Ezequiel, Jahn, Dieter

“…The Bacillus megaterium protein production system based on the inducible promoter of the xyl operon (PxylA) was systematically optimized. Multiple changes in…”
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Secretory pathway optimization of CHO producer cells by co-engineering of the mitosRNA-1978 target genes CerS2 and Tbc1D20 by Pieper, Lisa A., Strotbek, Michaela, Wenger, Till, Gamer, Martin, Olayioye, Monilola A., Hausser, Angelika

“…Chinese Hamster Ovary (CHO) cells are the most commonly used host for the production of biopharmaceuticals. Although transcription and translation engineering…”
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Rational optimization of a monoclonal antibody improves the aggregation propensity and enhances the CMC properties along the entire pharmaceutical process chain by Bauer, Joschka, Mathias, Sven, Kube, Sebastian, Otte, Kerstin, Garidel, Patrick, Gamer, Martin, Blech, Michaela, Fischer, Simon, Karow-Zwick, Anne R

“…The discovery of therapeutic monoclonal antibodies (mAbs) primarily focuses on their biological activity favoring the selection of highly potent drug…”
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High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium by Yang, Yang, Biedendieck, Rebekka, Wang, Wei, Gamer, Martin, Malten, Marco, Jahn, Dieter, Deckwer, Wolf-Dieter

“…During the last years B. megaterium was continuously developed as production host for the secretion of proteins into the growth medium. Here, recombinant…”
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A Bacillus megaterium Plasmid System for the Production, Export, and One-Step Purification of Affinity-Tagged Heterologous Levansucrase from Growth Medium by MALTEN, Marco, BIEDENDIECK, Rebekka, GAMER, Martin, DREWS, Ann-Christin, STAMMEN, Simon, BUCHHOLZ, Klaus, DIJKHUIZEN, Lubbert, JAHN, Dieter

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Structural analysis of random transgene integration in CHO manufacturing cell lines by targeted sequencing by Stadermann, Anna, Gamer, Martin, Fieder, Jürgen, Lindner, Benjamin, Fehrmann, Steffen, Schmidt, Moritz, Schulz, Patrick, Gorr, Ingo H.

“…Genetically modified CHO cell lines are traditionally used for the production of biopharmaceuticals. However, an in‐depth molecular understanding of the…”
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miRNA engineering of CHO cells facilitates production of difficult‐to‐express proteins and increases success in cell line development by Fischer, Simon, Marquart, Kim F., Pieper, Lisa A., Fieder, Juergen, Gamer, Martin, Gorr, Ingo, Schulz, Patrick, Bradl, Harald

“…ABSTRACT In recent years, coherent with growing biologics portfolios also the number of complex and thus difficult‐to‐express (DTE) therapeutic proteins has…”
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A blueprint from nature: miRNome comparison of plasma cells and CHO cells to optimize therapeutic antibody production by Raab, Nadja, Zeh, Nikolas, Schlossbauer, Patrick, Mathias, Sven, Lindner, Benjamin, Stadermann, Anna, Gamer, Martin, Fischer, Simon, Holzmann, Karlheinz, Handrick, René, Otte, Kerstin

“…•A miRNome analysis of CHO and plasma cell lines identified 147 signature miRNAs.•Productivity of CHO cells was optimized by 14 characteristic plasma cell…”
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Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy by Mathias, Sven, Fischer, Simon, Handrick, René, Fieder, Jürgen, Schulz, Patrick, Bradl, Harald, Gorr, Ingo, Gamer, Martin, Otte, Kerstin

“…[Display omitted] •A method examining a recombinant proteins intracellular distribution is presented.•For a bispecific antibody the ER was shown to be the most…”
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Loss of a newly discovered microRNA in Chinese hamster ovary cells leads to upregulation of N‐glycolylneuraminic acid sialylation on monoclonal antibodies by Fischer, Simon, Mathias, Sven, Stadermann, Anna, Yang, Shumin, Schmieder, Valerie, Zeh, Nikolas, Schmidt, Nicoletta, Richter, Patrick, Wright, Sara, Zimmermann, Eike, Ley, Yan, Meer, Julia, Hartsch, Thomas, Bernloehr, Christian, Otte, Kerstin, Bradl, Harald, Gamer, Martin, Schulz, Patrick

“…Chinese hamster ovary (CHO) cells are known not to express appreciable levels of the sialic acid residue N‐glycolylneuraminic acid (NGNA) on monoclonal…”
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Impact of rare codons and the functional coproduction of rate-limiting tRNAs on recombinant protein production in Bacillus megaterium by Finger, Constanze, Gamer, Martin, Klunkelfuß, Saskia, Bunk, Boyke, Biedendieck, Rebekka

“…The Gram-positive bacterium Bacillus megaterium was systematically developed for the plasmid-based production of recombinant proteins at the gram-per-liter…”
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T7 RNA polymerase-dependent gene expression system for Bacillus megaterium by Gamer, Martin, Fröde, David, Biedendieck, Rebekka, Stammen, Simon, Jahn, Dieter

“…Gene expression systems based on the RNA polymerase of the bacteriophage T7 are often the ultimate choice for the high level production of recombinant…”
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Looking for natural competence in Bacillus megaterium by Knuuti, Tobias, Gamer, Martin, Finger, Constanze, Biedendieck, Rebekka, Jahn, Dieter

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Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium by Stammen, Simon, Schuller, Franziska, Dietrich, Sylvia, Gamer, Martin, Biedendieck, Rebekka, Jahn, Dieter

“…Gene “7” of Escherichia coli phage K1E was proposed to encode a novel DNA-dependent RNA polymerase (RNAP). The corresponding protein was produced…”
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A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium by Biedendieck, Rebekka, Gamer, Martin, Jaensch, Lothar, Meyer, Steffen, Rohde, Manfred, Deckwer, Wolf-Dieter, Jahn, Dieter

“…A sucrose-inducible promoter system (P sacB ) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the…”
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Influence of codon usage on heterologous protein production in Bacillus megaterium by Finger, Constanze, Gamer, Martin, Bunk, Boyke, Knuuti, Tobias, Biedendieck, Rebekka, Jahn, Dieter

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Export, purification, and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium by Biedendieck, Rebekka, Beine, Rafael, Gamer, Martin, Jordan, Eva, Buchholz, Klaus, Seibel, Jürgen, Dijkhuizen, Lubbert, Malten, Marco, Jahn, Dieter

“…Fructosyltransferases, like the Lactobacillus reteri levansucrase, are important for the production of new fructosyloligosaccharides. Various His₆- and…”
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A sucrose-inducible promoter system for the intra-and extracellular protein production in Bacillus megaterium: In memoriam W.-D. Deckwer: Merging process engineering and systems biology by BIEDENDIECK, Rebekka, GAMER, Martin, JAENSCH, Lothar, MEYER, Steffen, ROHDE, Manfred, DECKWER, Wolf-Dieter, JAHN, Dieter

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