Genome-Wide Analysis and Molecular Characterization of Orf Virus Strain UPM/HSN-20 Isolated From Goat in Malaysia

Contagious ecthyma commonly known as Orf is a globally important, highly contagious zoonotic, transboundary disease that affects domestic and wild ruminants. The disease is of great economic significance causing an immense impact on animal health, welfare, productivity, and trade. Detailed analysis...

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Bibliographic Details
Published in:	Frontiers in microbiology Vol. 13; p. 877149
Main Authors:	Mangga, Hassana Kyari, Bala, Jamilu Abubakar, Balakrishnan, Krishnan Nair, Bukar, Alhaji Modu, Lawan, Zaharaddeen, Gambo, Auwal, Jesse, Faez Firdaus Abdullah, Noordin, Mustapha M., Mohd-Azmi, Mohd-Lila
Format:	Journal Article
Language:	English
Published:	Frontiers Media S.A 11-07-2022
Subjects:	cell culture genome Microbiology next-generation sequencing Orf virus ruminants
Online Access:	Get full text
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Summary:	Contagious ecthyma commonly known as Orf is a globally important, highly contagious zoonotic, transboundary disease that affects domestic and wild ruminants. The disease is of great economic significance causing an immense impact on animal health, welfare, productivity, and trade. Detailed analysis of the viral genome is crucial to further elucidate the molecular mechanism of Orf virus (ORFV) pathogenesis. In the present study, a confluent monolayer of lamb testicle cells was infected with the processed scab sample obtained from an infected goat. The presence of the virus was confirmed using polymerase chain reaction and electron microscopy, while its genome was sequenced using next-generation sequencing technology. The genome sequence of Malaysian ORFV strain UPM/HSN-20 was found to contain 132,124 bp with a G + C content of 63.7%. The homology analysis indicates that UPM/HSN-20 has a high level of identity 97.3–99.0% with the other reference ORFV strain. Phylogenetic analysis revealed that ORFV strain UPM/HSN-20 is genetically more closely related to ORFV strain XY and NP from China. The availability of the genome-wide analysis of ORFV UPM/HSN-20 strain from Malaysia will serve as a good platform for further understanding of genetic diversity, ORFV infection, and strategic development for control measures.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Virology, a section of the journal Frontiers in Microbiology Edited by: Erna Geessien Kroon, Federal University of Minas Gerais, Brazil Reviewed by: Andrea Peralta, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Lauro Velazquez-Salinas, United States Department of Agriculture, United States
ISSN:	1664-302X 1664-302X
DOI:	10.3389/fmicb.2022.877149