Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

There is scarce information concerning the role of sporadic clones in the dissemination of antimicrobial resistance genes (ARGs) within the nosocomial niche. We confirmed that the clinical M19736 ST615 strain, one of the first isolates of Latin America that harbors a plasmid with an gene, could rece...

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Published in:	Frontiers in cellular and infection microbiology Vol. 14; p. 1368622
Main Authors:	Carrera Páez, Laura Camila, Olivier, Martin, Gambino, Anahí Samanta, Poklepovich, Tomás, Aguilar, Andrea Pamela, Quiroga, María Paula, Centrón, Daniela
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 29-04-2024
Subjects:	Anti-Bacterial Agents - pharmacology antimicrobial resistance beta-Lactamases - genetics Cellular and Infection Microbiology conjugation Conjugation, Genetic Drug Resistance, Bacterial - genetics Drug Resistance, Multiple, Bacterial - genetics Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics extracellular vesicles (EVs) Gene Transfer, Horizontal Humans Latin America mcr-1 gene Microbial Sensitivity Tests Plasmids - genetics Latin America extracellular vesicles (EVs) antimicrobial resistance conjugation Escherichia coli mcr-1 gene
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Summary:	There is scarce information concerning the role of sporadic clones in the dissemination of antimicrobial resistance genes (ARGs) within the nosocomial niche. We confirmed that the clinical M19736 ST615 strain, one of the first isolates of Latin America that harbors a plasmid with an gene, could receive crucial ARG by transformation and conjugation using as donors critical plasmids that harbor , , , , or genes. M19736 acquired , , , , and genes, being only blaNDM-1 maintained at 100% on the 10th day of subculture. In addition, when the evolved MDR- M19736 acquired sequentially and genes, the maintenance pattern of the plasmids changed. In addition, when the evolved XDR- M19736 acquired in an ulterior step the paadB plasmid, a different pattern of the plasmid's maintenance was found. Interestingly, the evolved M19736 strains disseminated simultaneously the acquired conjugative plasmids in different combinations though selection was ceftazidime in all cases. Finally, we isolated and characterized the extracellular vesicles (EVs) from the native and evolved XDR- M19736 strains. Interestingly, EVs from the evolved XDR- M19736 harbored though the pDCAG1-CTX-M-15 was previously lost as shown by WGS and experiments, suggesting that EV could be a relevant reservoir of ARG for susceptible bacteria. These results evidenced the genetic plasticity of a sporadic clone of such as ST615 that could play a relevant transitional link in the clinical dynamics and evolution to multidrug/extensively/pandrug-resistant phenotypes of superbugs within the nosocomial niche by acting simultaneously as a vector and reservoir of multiple ARGs which later could be disseminated.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Angel Adrián Cataldi, Instituto Nacional de Tecnología Agropecuaria, Argentina Pablo Power, Universidad de Buenos Aires, Argentina Reviewed by: Marina Rosa Pulido, University of Seville, Spain
ISSN:	2235-2988 2235-2988
DOI:	10.3389/fcimb.2024.1368622