Search Results - "Gait, M. J."

Cell penetrating peptides: overview and applications to the delivery of oligonucleotides by Said Hassane, F, Saleh, A. F, Abes, R, Gait, M. J, Lebleu, Bernard

“…Crossing biological barriers represents a major limitation for clinical applications of biomolecules such as nucleic acids, peptides or proteins. Cell…”
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Conjugates of oligonucleotides and analogues with cell penetrating peptides as gene silencing agents by Zatsepin, T S, Turner, J J, Oretskaya, T S, Gait, M J

“…The review describes key aspects of the synthesis and biological activities of conjugates of oligonucleotides and their analogues with synthetic peptides, in…”
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Inhibition of HIV-1 Tat-Dependent Trans Activation by Steric Block Chimeric 2‘-O-Methyl/LNA Oligoribonucleotides by Arzumanov, Andrey, Walsh, Andrew P, Rajwanshi, Vivek K, Kumar, Ravindra, Wengel, Jesper, Gait, Michael J

“…The HIV-1 trans-activation responsive element (TAR) RNA 59-residue stem−loop interacts with the HIV trans-activator protein Tat and other cellular factors to…”
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Human Immunodeficiency Virus 1 Tat Protein Binds Trans-Activation-Responsive Region (TAR) RNA in vitro by Dingwall, Colin, Ernberg, Ingemar, Gait, Michael J., Green, Sheila M., Heaphy, Shaun, Karn, Jonathan, Lowe, Anthony D., Singh, Mohinder, Skinner, Michael A., Valerio, Robert

“…tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds…”
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HIV‐1 tat protein stimulates transcription by binding to a U‐rich bulge in the stem of the TAR RNA structure by Dingwall, C., Ernberg, I., Gait, M. J., Green, S. M., Heaphy, S., Karn, J., Lowe, A. D., Singh, M., Skinner, M. A.

“…The HIV‐1 trans‐activator protein, tat, is an RNA binding protein with a high affinity for a U‐rich bulge near the tip of the stem in the RNA stem‐loop…”
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RNA-targeted splice-correction therapy for neuromuscular disease by Wood, Matthew J. A., Gait, Michael J., Yin, Haifang

“…Splice-modulation therapy, whereby molecular manipulation of premessenger RNA splicing is engineered to yield genetic correction, is a promising novel therapy…”
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A Convenient Solid-Phase Method for Synthesis of 3‘-Conjugates of Oligonucleotides by Stetsenko, Dmitry A, Gait, Michael J

“…We present a new procedure for the preparation of 3‘-conjugates of oligonucleotides through solid-phase synthesis. A suitable universal solid support was…”
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Configurationally defined phosphorothioate-containing oligoribonucleotides in the study of the mechanism of cleavage of hammerhead ribozymes by Slim, G, Gait, M.J

“…The chemical synthesis is described of oligoribonucleotides containing a single phosphorothioate linkage of defined Rp and Sp configuration. The…”
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Human Immunodeficiency Virus Type 1 Regulator of Virion Expression, Rev, Forms Nucleoprotein Filaments After Binding to a Purine-Rich "bubble" Located within the Rev-Responsive Region of Viral mRNAs by Heaphy, Shaun, Finch, John T., Gait, Michael J., Karn, Jonathan, Singh, Mohinder

“…The human immunodeficiency virus type 1 rev protein binds with high affinity (K$_d < 1-3$nM) to a purine-rich "bubble" containing bulged GG and GUA residues on…”
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Cell-penetrating-peptide-based delivery of oligonucleotides: an overview by Abes, R, Arzumanov, A A, Moulton, H M, Abes, S, Ivanova, G D, Iversen, P L, Gait, M J, Lebleu, B

“…Cationic CPPs (cell-penetrating peptides) have been used largely for intracellular delivery of low-molecular-mass drugs, biomolecules and particles. Most…”
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Base and Sugar Requirements for RNA Cleavage of Essential Nucleoside Residues in Internal Loop B of the Hairpin Ribozyme: Implications for Secondary Structure by Schmidt, Sabine, Beigelman, Leonid, Karpeisky, Alexander, Usman, Nassim, Sørensen, Ulrik S., Gait, Michael J.

“…The hairpin ribozyme is a small self-cleaving RNA that can be engineered for RNA cleavage in trans and has potential as a therapeutic agent. We have used a…”
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High affinity binding of TAR RNA by the human immunodeficiency virus type-1 tat protein requires base-pairs in the RNA stem and amino acid residues flanking the basic region by Churcher, M J, Lamont, C, Hamy, F, Dingwall, C, Green, S M, Lowe, A D, Butler, J G, Gait, M J, Karn, J

“…The binding site for tat protein on TAR RNA has been defined in quantitative terms using an extensive series of mutations. The relative dissociation constants…”
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ARP, a peptide derived from the stress-associated acetylcholinesterase variant, has hematopoietic growth promoting activities by Grisaru, D, Deutsch, V, Shapira, M, Pick, M, Sternfeld, M, Melamed-Book, N, Kaufer, D, Galyam, N, Gait, M J, Owen, D, Lessing, J B, Eldor, A, Soreq, H

“…Psychological stress induces rapid and long-lasting changes in blood cell composition, implying the existence of stress-induced factors that modulate…”
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Human Immunodeficiency Virus Type-1 Tat is an Integral Component of the Activated Transcription-Elongation Complex by Keen, Nicholas J., Gait, Michael J., Karn, Jonathan

“…The human immunodeficiency virus type 1 transactivator protein, Tat, stimulates transcriptional elongation from the viral long terminal repeat. To test whether…”
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Progress in anti-HIV structure-based drug design by Gait, Michael J., Karn, Jonathan

“…The course of drug development for the treatment of HIV-1 infection and AIDS is being revolutionized by high-resolution structures of essential viral proteins…”
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Arginine-rich cell penetrating peptides: Design, structure-activity, and applications to alter pre-mRNA splicing by steric-block oligonucleotides by Abes, R., Arzumanov, A., Moulton, H., Abes, S., Ivanova, G., Gait, M. J., Iversen, P., Lebleu, B.

“…Rerouting the splicing machinery with steric‐block oligonucleotides (ON) might lead to new therapeutic strategies in the treatment of diseases such as…”
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Nucleotide Sequence of Tobacco Mosaic Virus RNA by Goelet, P., Lomonossoff, G. P., Butler, P. J. G., Akam, M. E., Gait, M. J., Karn, J.

“…Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has…”
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Human Immunodeficiency Virus Type 1 Transactivator Protein, Tat, Stimulates Transcriptional Read-Through of Distal Terminator Sequences in vitro by Graeble, Maria A., Churcher, Mark J., Lowe, Anthony D., Gait, Michael J., Karn, Jonathan

“…The human immunodeficiency virus type 1 transactivator protein, tat, specifically stimulates transcription from the viral long terminal repeat. We used…”
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Recognition of the high affinity binding site in rev-response element RNA by the Human Immunodeficiency Virus type-1 rev protein by Iwai, Shigenori, Pritchard, Clare, Mann, Derek A., Karn, Jonathan, Gait, Michael J.

“…The Human Immunodeficiency Virus type-1 rev protein binds with high affinity to a bubble structure located within the rev-response element (RRE) RNA in…”
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OLIGONUCLEOTIDE ANALOGUE INTERFERENCE WITH THE HIV-1 TAT PROTEIN-TAR RNA INTERACTION by Arzumanov, Andrey, Walsh, Andrew P., Liu, Xiaohai, Rajwanshi, Vivek K., Wengel, Jesper, Gait, Michael J.

“…The HIV-1 Tat protein interaction with its RNA recognition sequence TAR is an important drug target and model system for the development of specific…”
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