Search Results - "Gaigalas, A."

Measurement of Scattering and Absorption Cross Sections of Microspheres for Wavelengths between 240 nm and 800 nm by Gaigalas, A K, Wang, Lili, Choquette, Steven

“…A commercial spectrometer with a 150 mm integrating sphere (IS) detector was used to estimate the scattering and absorption cross sections of monodisperse…”
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Measurement of Microsphere Concentration Using a Flow Cytometer with Volumetric Sample Delivery by Wang, Lili, Zhang, Yu-Zhong, Choquette, Steven, Gaigalas, A K

“…Microsphere concentrations are needed to assign equivalent reference fluorophores (ERF) units to microspheres used in quantitative flow cytometry. A flow…”
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Raman and FTIR spectroscopies of fluorescein in solutions by Wang, Lili, Roitberg, A., Meuse, C., Gaigalas, A.K.

“…Raman and Fourier transform-infra red (FT-IR) spectroscopies of fluorescein in aqueous solutions have been investigated in the pH range from 9.1 to 5.4. At pH…”
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Measurement of Absorption and Scattering With an Integrating Sphere Detector: Application to Microalgae by Gaigalas, A K, He, Hua-Jun, Wang, Lili

“…A spectrometer with an integrating sphere (IS) detector was used to measure the absorbance due to scattering and absorption. Analysis of the measurement…”
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Procedures for Wavelength Calibration and Spectral Response Correction of CCD Array Spectrometers by Gaigalas, A K, Wang, Lili, He, Hua-Jun, DeRose, Paul

“…This work describes a procedure for acquiring a spectrum of an analyte over an extended range of wavelengths and validating the wavelength and intensity…”
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Quantification of cells with specific phenotypes II: Determination of CD4 expression level on reconstituted lyophilized human PBMC labelled with anti‐CD4 FITC antibody by Wang, L., Stebbings, R., Gaigalas, A. K., Sutherland, J., Kammel, M., John, M., Roemer, B., Kuhne, M., Schneider, R. J., Braun, M., Engel, A., Dikshit, D., Abbasi, F., Marti, G. E., Sassi, M., Revel, L., Kim, S. K., Baradez, M., Lekishvili, T., Marshall, D., Whitby, L., Jing, W., Ost, V., Vonsky, M., Neukammer, J.

“…This report focuses on the characterization of CD4 expression level in terms of equivalent number of reference fluorophores (ERF). Twelve different flow…”
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Measurement of Scattering Cross Section with a Spectrophotometer with an Integrating Sphere Detector by Gaigalas, A K, Wang, Lili, Karpiak, V, Zhang, Yu-Zhong, Choquette, Steven

“…A commercial spectrometer with an integrating sphere (IS) detector was used to measure the scattering cross section of microspheres. Analysis of the…”
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A Multistate Model for the Fluorescence Response of R-Phycoerythrin by Gaigalas, A., Gallagher, T., Cole, K. D., Singh, T., Wang, Lili, Zhang, Yu-Zhong

“…Although strong fluorescence makes the R-phycoerythrin (R-PE) proteins increasingly useful in biological and clinical assays, they are subject to nonlinear…”
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Fluorescent Nanometer Microspheres as a Reporter for Sensitive Detection of Simulants of Biological Threats Using Multiplexed Suspension Arrays by Wang, L, Cole, K. D, Gaigalas, A. K, Zhang, Yu-Zhong

“…We succeeded in using 40 nm FRET (fluorescence resonance energy transfer) microspheres conjugated to antibodies as the fluorescent reporters to perform the…”
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Monoclonal Antibody Selection for Interleukin-4 Quantification Using Suspension Arrays and Forward-Phase Protein Microarrays by Wang, L, Cole, K. D, Peterson, A, He, Hua-Jun, Gaigalas, A. K, Zong, Y

“…A recombinant mouse interleukin-4 (IL-4) and three different purified rat antimouse IL-4 monoclonal antibodies (Mab) with different clonalities were employed…”
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Photodegradation of Fluorescein in Solutions Containing n-Propyl Gallate by Gaigalas, A. K, Wang, Lili, Cole, K. D, Humphries, E

“…The frequency domain technique was applied to measure the effect of n-propyl gallate (nPG) on the apparent photodegradation rate of fluorescein in aqueous…”
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The effect of overhanging nucleotides on fluorescence properties of hybridising oligonucleotides labelled with Alexa-488 and FAM fluorophores by Noble, J.E., Wang, L., Cole, K.D., Gaigalas, A.K.

“…In order to rationally select and design probes for real-time PCR, we have determined the influence of the overhang region of the complementary strand on the…”
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Comparison of Ovalbumin Quantification Using Forward-Phase Protein Microarrays and Suspension Arrays by Wang, L, Cole, K. D, He, Hua-Jun, Hancock, D. K, Gaigalas, A. K, Zong, Y

“…We employed ovalbumin (a simulant used for ricin and botulism toxins in biodefense applications) and its high affinity polyclonal antibody as a model system to…”
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Photophysical Properties of Ricin by Gaigalas, A.K., Cole, K.D., Bykadi, S., Wang, Lili, DeRose, Paul

“…ABSTRACT The molar absorption coefficient of ricin in phosphate‐buffered saline (PBS) at 279 nm was measured as (93 900 ± 3300) L mol−1 cm−1. The concentration…”
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The Development of Fluorescence Intensity Standards by Gaigalas, A K, Li, L, Henderson, O, Vogt, R, Barr, J, Marti, G, Weaver, J, Schwartz, A

“…The use of fluorescence as an analytical technique has been growing over the last 20 years. A major factor in inhibiting more rapid growth has been the…”
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Spectroscopic characterization of fluorescein- and tetramethylrhodamine-labeled oligonucleotides and their complexes with a DNA template by Wang, L., Gaigalas, A.K., Blasic, J., Holden, M.J.

“…We measured absorption and emission spectra, fluorescence quantum yield, anisotropy, fluorescence resonance energy transfer (FRET), and melting temperature to…”
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Quantitating Fluorescence Intensity From Fluorophore: Assignment of MESF Values by Gaigalas, A K, Wang, Lili, Schwartz, Abe, Marti, Gerald E, Vogt, Jr, Robert F

“…A procedure is presented to convert the comparison of measured fluorescence signals into a comparison of fluorescence yields (FY). The fluorescence yield,…”
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Quantitating Fluorescence Intensity from Fluorophore: The Definition of MESF Assignment by Schwartz, Abe, Wang, Lili, Early, Edward, Gaigalas, Adolfas, Zhang, Yu-Zhong, Marti, Gerald E, Vogt, Robert F

“…The quantitation of fluorescence radiance may at first suggest the need to obtain the number of fluorophore that are responsible for the measured fluorescence…”
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Evaluating the quality of data from DNA microarray measurements by Wang, Lili, Gaigalas, A K, Satterfield, M B, Salit, M, Zong, Y, Noble, J

“…Gene expression technology offers great potentials to generate new insights into human disease pathogenesis; however, the data quality remains a major obstacle…”
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Fluorescence resonance energy transfer between donor-acceptor pair on two oligonucleotides hybridized adjacently to DNA template by Wang, L., Gaigalas, A. K., Blasic, J., Holden, M. J., Gallagher, D. T., Pires, R.

“…We use fluorescein as the energy donor and rhodamine as the acceptor to measure the efficiency of fluorescence resonance energy transfer (FRET) in a set of…”
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