Search Results - "Gadella, Theodorus W. J"

mScarlet: a bright monomeric red fluorescent protein for cellular imaging by Bindels, Daphne S, Haarbosch, Lindsay, van Weeren, Laura, Postma, Marten, Wiese, Katrin E, Mastop, Marieke, Aumonier, Sylvain, Gotthard, Guillaume, Royant, Antoine, Hink, Mark A, Gadella, Theodorus W J

“…An extremely bright, truly monomeric RFP, mScarlet, is described that outperforms existing RFPs in diverse labeling applications, especially in FRET with…”
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Breaking up the StayGold dimer yields three photostable monomers by Goedhart, Joachim, Gadella, Theodorus W. J.

“…The exceptionally photostable green fluorescent protein StayGold has been monomerized in different laboratories, which has generated three unique monomeric…”
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Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2 by Mastop, Marieke, Bindels, Daphne S., Shaner, Nathan C., Postma, Marten, Gadella, Theodorus W. J., Goedhart, Joachim

“…The performance of Förster Resonance Energy Transfer (FRET) biosensors depends on brightness and photostability, which are dependent on the characteristics of…”
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A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium by van der Linden, Franka H., Mahlandt, Eike K., Arts, Janine J. G., Beumer, Joep, Puschhof, Jens, de Man, Saskia M. A., Chertkova, Anna O., Ponsioen, Bas, Clevers, Hans, van Buul, Jaap D., Postma, Marten, Gadella, Theodorus W. J., Goedhart, Joachim

“…The most successful genetically encoded calcium indicators (GECIs) employ an intensity or ratiometric readout. Despite a large calcium-dependent change in…”
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A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range by Klarenbeek, Jeffrey B, Goedhart, Joachim, Hink, Mark A, Gadella, Theodorus W J, Jalink, Kees

“…FRET-based sensors for cyclic Adenosine Mono Phosphate (cAMP) have revolutionized the way in which this important intracellular messenger is studied. The…”
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Bright cyan fluorescent protein variants identified by fluorescence lifetime screening by Gadella, Theodorus W J, Goedhart, Joachim, van Weeren, Laura, Hink, Mark A, Vischer, Norbert O E, Jalink, Kees

“…Optimization of autofluorescent proteins by intensity-based screening of bacteria does not necessarily identify the brightest variant for eukaryotes. We report…”
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Multiparameter screening method for developing optimized red-fluorescent proteins by Bindels, Daphne S., Postma, Marten, Haarbosch, Lindsay, van Weeren, Laura, Gadella, Theodorus W. J.

“…Genetically encoded fluorescent proteins (FPs) are highly utilized in cell biology research to study proteins of interest or signal processes using biosensors…”
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mScarlet3: a brilliant and fast-maturing red fluorescent protein by Gadella, Theodorus W. J., van Weeren, Laura, Stouthamer, Jente, Hink, Mark A., Wolters, Anouk H. G., Giepmans, Ben N. G., Aumonier, Sylvain, Dupuy, Jérôme, Royant, Antoine

“…We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness,…”
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A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells by van Unen, Jakobus, Stumpf, Anette D, Schmid, Benedikt, Reinhard, Nathalie R, Hordijk, Peter L, Hoffmann, Carsten, Gadella, Jr, Theodorus W J, Goedhart, Joachim

“…G-protein coupled receptors (GPCRs) can activate a heterotrimeric G-protein complex with subsecond kinetics. Genetically encoded biosensors based on Förster…”
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SCARECROW‐LIKE23 and SCARECROW jointly specify endodermal cell fate but distinctly control SHORT‐ROOT movement by Long, Yuchen, Goedhart, Joachim, Schneijderberg, Martinus, Terpstra, Inez, Shimotohno, Akie, Bouchet, Benjamin P, Akhmanova, Anna, Gadella, Theodorus W. J., Jr, Heidstra, Renze, Scheres, Ben, Blilou, Ikram

“…Intercellular signaling through trafficking of regulatory proteins is a widespread phenomenon in plants and can deliver positional information for the…”
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Direct interactions of early and late assembling division proteins in Escherichia coli cells resolved by FRET by Alexeeva, Svetlana, Gadella, Theodorus W.J. Jr, Verheul, Jolanda, Verhoeven, Gertjan S, den Blaauwen, Tanneke

“…The bacterial cell division machinery is organized in the so-called divisome composed of highly dynamic but low abundant interacting (membrane-bound) proteins…”
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Cyan and Yellow Super Fluorescent Proteins with Improved Brightness, Protein Folding, and FRET Förster Radius by Kremers, Gert-Jan, Goedhart, Joachim, van Munster, Erik B, Gadella, Theodorus W. J

“…Enhanced cyan and yellow fluorescent proteins are widely used for dual color imaging and protein−protein interaction studies based on fluorescence resonance…”
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Imaging phosphatidylinositol 4‐phosphate dynamics in living plant cells by Vermeer, Joop E.M., Thole, Julie M., Goedhart, Joachim, Nielsen, Erik, Munnik, Teun, Gadella Jr, Theodorus W.J.

“…Summary Polyphosphoinositides represent a minor group of phospholipids, accounting for less than 1% of the total. Despite their low abundance, these molecules…”
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A local VE-cadherin and Trio-based signaling complex stabilizes endothelial junctions through Rac1 by Timmerman, Ilse, Heemskerk, Niels, Kroon, Jeffrey, Schaefer, Antje, van Rijssel, Jos, Hoogenboezem, Mark, van Unen, Jakobus, Goedhart, Joachim, Gadella, Jr, Theodorus W J, Yin, Taofei, Wu, Yi, Huveneers, Stephan, van Buul, Jaap D

“…Endothelial cell-cell junctions maintain a restrictive barrier that is tightly regulated to allow dynamic responses to permeability-inducing angiogenic…”
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Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes by Dhonukshe, Pankaj, Grigoriev, Ilya, Fischer, Rainer, Tominaga, Motoki, Robinson, David G, Hašek, Jiří, Paciorek, Tomasz, Petrášek, Jan, Seifertová, Daniela, Tejos, Ricardo, Meisel, Lee A, Zažímalová, Eva, Gadella, Theodorus W.J. Jr, Stierhof, York-Dieter, Ueda, Takashi, Oiwa, Kazuhiro, Akhmanova, Anna, Brock, Roland, Spang, Anne, Friml, Jiří

“…Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule…”
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Quantitative Determination of Dark Chromophore Population Explains the Apparent Low Quantum Yield of Red Fluorescent Proteins by Prangsma, Jord C, Molenaar, Robert, van Weeren, Laura, Bindels, Daphne S, Haarbosch, Lindsay, Stouthamer, Jente, Gadella, Theodorus W. J, Subramaniam, Vinod, Vos, Willem L, Blum, Christian

“…The fluorescence quantum yield of four representative red fluorescent proteins mCherry, mKate2, mRuby2, and the recently introduced mScarlet was investigated…”
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Visualization of phosphatidylinositol 4,5-bisphosphate in the plasma membrane of suspension-cultured tobacco BY-2 cells and whole Arabidopsis seedlings by van Leeuwen, Wessel, Vermeer, Joop E.M, Gadella, Theodorus W.J. Jr, Munnik, Teun

“…Phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P₂] is an important signalling lipid in mammalian cells, where it functions as a second-messenger precursor…”
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Real-time visualization of heterotrimeric G protein Gq activation in living cells by Adjobo-Hermans, Merel J W, Goedhart, Joachim, van Weeren, Laura, Nijmeijer, Saskia, Manders, Erik M M, Offermanns, Stefan, Gadella, Jr, Theodorus W J

“…Gq is a heterotrimeric G protein that plays an important role in numerous physiological processes. To delineate the molecular mechanisms and kinetics of…”
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Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples by Goedhart, Joachim, Vermeer, Joop E M, Adjobo-Hermans, Merel J W, van Weeren, Laura, Gadella, Jr, Theodorus W J

“…Fluorescence Resonance Energy Transfer (FRET) between the green fluorescent protein (GFP) variants CFP and YFP is widely used for the detection of…”
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Quantitative co-expression of proteins at the single cell level--application to a multimeric FRET sensor by Goedhart, Joachim, van Weeren, Laura, Adjobo-Hermans, Merel J W, Elzenaar, Ies, Hink, Mark A, Gadella, Jr, Theodorus W J

“…Co-expression of proteins is generally achieved by introducing two (or more) independent plasmids into cells, each driving the expression of a different…”
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