UV-B Induced stimulation of phosphatidylinositol 3-kinase in human fetal hepatocytes

We investigated the effect of UV-B irradiation on PI 3-kinase activity in human fetal hepatocytes. When cells were exposed to monochromatic (304nm) UV-B light, a significant increase in intracellular PI 3-kinase activity was observed in a dose dependent manner with maximal activity upon 1500 Jm(-2)...

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Bibliographic Details
Published in:	Indian journal of clinical biochemistry Vol. 19; no. 1; pp. 118 - 121
Main Authors:	Chari, V B, Gaddameedi, R S R, Singh, M Raghuveer, Kasina, Sathish, Rahman, Khaja Khaleel Ur, Habibullah, C M, Singh, Surya S
Format:	Journal Article
Language:	English
Published:	India Springer Nature B.V 01-01-2004 Springer India
Subjects:	1-Phosphatidylinositol 3-kinase Cell survival Cell viability Fetuses Hepatocytes Lipid kinase Proteins Transplantation Wortmannin Signal transduction Lipid kinase Hepatocytes transplantation Xenotransplantation
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Summary:	We investigated the effect of UV-B irradiation on PI 3-kinase activity in human fetal hepatocytes. When cells were exposed to monochromatic (304nm) UV-B light, a significant increase in intracellular PI 3-kinase activity was observed in a dose dependent manner with maximal activity upon 1500 Jm(-2) irradiation. At 1500 Jm(-2) dose PI 3-kinse activity increased by 80% in membrane fraction of fetal hepatocytes of 25 weeks gestation. PI 3-kinse inhibitors wortmannin and LY294002 specifically inhibited the UV-B induced lipid kinase activity and blocked significantly the UV-B induced cell viability. The data suggests a correlation between cell survival and elevated levels of PI 3-kinase and suggest that UV-B irradiation at a dose of 1500 Jm(-2) is ideal for fetal hepatocyte transplantation. Also, PI 3-kinase levels could be a representative marker for viable UV-B irradiated fetal hepatocytes for transplantation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0970-1915 0974-0422
DOI:	10.1007/BF02872405