Flexible, Biocompatible PET Sheets: A Platform for Attachment, Proliferation and Differentiation of Eukaryotic Cells

Flexible, Biocompatible PET Sheets: A Platform for Attachment, Proliferation and Differentiation of Eukaryotic Cells

Transparent, flexible, biaxially oriented polyethylene terephthalate (PET) sheets were modified by bioactive polymer-fibronectin top layers for the attachment of cells and growth of muscle fibers. Towards this end, PET sheets were grafted with 4-(dimethylamino)phenyl (DMA) groups from the in situ ge...

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Bibliographic Details
Published in:Surfaces (Basel) Vol. 4; no. 4; pp. 306 - 322
Main Authors: Samanta, Soumen, Gaad, Diana, Cabet, Eva, Lilienbaum, Alain, Singh, Ajay, Aswal, Dinesh K., Chehimi, Mohamed M.
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-12-2021
Subjects:
Attachment
Biocompatibility
biocompatible polymers
Biological activity
Biomedical materials
Carbonyls
cell adhesion
Crosslinking
Differentiation
Ethanol
Ethylene glycol
eukaryotic cells
Extracellular matrix
Fibers
Fibronectin
flexible supports
Free radicals
Glycol dimethacrylates
Grafting
muscle fibers
Muscles
Photopolymerization
Polyethylene terephthalate
Polymers
Proteins
Sheets
Germany
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Summary:Transparent, flexible, biaxially oriented polyethylene terephthalate (PET) sheets were modified by bioactive polymer-fibronectin top layers for the attachment of cells and growth of muscle fibers. Towards this end, PET sheets were grafted with 4-(dimethylamino)phenyl (DMA) groups from the in situ generated diazonium cation precursor. The arylated sheets served as macro-hydrogen donors for benzophenone and the growth of poly(2-hydroxy ethyl methacrylate) (PHEMA) top layer by surface-confined free radical photopolymerization. The PET-PHEMA sheets were further grafted with fibronectin (FBN) through the 1,1-carbonyldiimidazole coupling procedure. The bioactive PET-PHEMA-I-FBN was then employed as a platform for the attachment, proliferation and differentiation of eukaryotic cells which after a few days gave remarkable muscle fibers, of ~120 µm length and ~45 µm thickness. We demonstrate that PET-PHEMA yields a fast growth of cells followed by muscle fibers of excellent levels of differentiation compared to pristine PET or standard microscope glass slides. The positive effect is exacerbated by crosslinking PHEMA chains with ethylene glycol dimethacrylate at initial HEMA/EGDA concentration ratio = 9/1. This works conclusively shows that in situ generated diazonium salts provide aryl layers for the efficient UV-induced grafting of biocompatible coating that beneficially serve as platform for cell attachment and growth of muscle fibers.
ISSN:2571-9637
2571-9637
DOI:10.3390/surfaces4040026