Search Results - "GRABER, S. G"

Expression and purification of functional G protein alpha subunits using a baculovirus expression system by Graber, S G, Figler, R A, Garrison, J C

“…The alpha subunits of the heterotrimeric guanine nucleotide-binding proteins Gi1, Gi2, Gi3, G0, and Gs have been overexpressed in Sf9 cells using a baculovirus…”
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Expression of functional G protein beta gamma dimers of defined subunit composition using a baculovirus expression system by Graber, S G, Figler, R A, Kalman-Maltese, V K, Robishaw, J D, Garrison, J C

“…The 36-kDa beta 1, 35-kDa beta 2, and 6.5-kDa gamma 2 subunits of the heterotrimeric guanine nucleotide-binding proteins have been overexpressed in Sf9 cells…”
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Myoglobin expression in L6 muscle cells. Role of differentiation and heme by Graber, S G, Woodworth, R C

“…Analysis of myoglobin levels in L6 cells (derived from rat skeletal muscle) by radioimmunoassay shows that myoglobin is not synthesized until after the cells…”
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Reconstitution of recombinant bovine A1 adenosine receptors in Sf9 cell membranes with recombinant G proteins of defined composition by Figler, R A, Graber, S G, Lindorfer, M A, Yasuda, H, Linden, J, Garrison, J C

“…We investigated the coupling of A1 adenosine receptors to recombinant G proteins. Recombinant baculoviruses were used to express bovine A1 adenosine receptors…”
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Human 5-HT1 Receptor Subtypes Exhibit Distinct G Protein Coupling Behaviors in Membranes from Sf9 Cells by Clawges, Heather M, Depree, Karyn M, Parker, Eric M, Graber, Stephen G

“…The G protein coupling behavior of four human 5-hydroxytryptamine receptor subtypes (5-HT1A, 5-HT1B, 5-HT1D, and 5-HT1E) has been studied in membranes from Sf9…”
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Expression of the rat D2 and D3 dopamine receptors in insect cells using the baculovirus system by Woodcock, C, Graber, S G, Rooney, B C, Strange, P G

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Selective activation of phospholipase C by recombinant G-protein alpha- and beta gamma-subunits by Boyer, J L, Graber, S G, Waldo, G L, Harden, T K, Garrison, J C

“…Receptor activation of phospholipase C (PLC) via G-proteins occurs by pertussis toxin-sensitive and toxin-insensitive signaling pathways. The alpha-subunits of…”
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Expression and purification of G-protein alpha subunits using baculovirus expression system by Graber, S G, Figler, R A, Garrison, J C

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Selective activation of phospholipase C by recombinant G-protein α- and βγ-subunits by BOYER, J. L, GRABER, S. G, WALDO, G. L, HARDEN, T. K, GARRISON, J. C

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Stabilization of the GDP-bound Conformation of Giα by a Peptide Derived from the G-protein Regulatory Motif of AGS3 by Peterson, Yuri K., Bernard, Michael L., Ma, Hongzheng, Hazard, Starr, Graber, Stephen G., Lanier, Stephen M.

“…The G-protein regulatory (GPR) motif in AGS3 was recently identified as a region for protein binding to heterotrimeric G-protein α subunits. To define the…”
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Agonist regulation of D(2) dopamine receptor/G protein interaction. Evidence for agonist selection of G protein subtype by Cordeaux, Y, Nickolls, S A, Flood, L A, Graber, S G, Strange, P G

“…The D(2) dopamine receptor has been expressed in Sf21 insect cells together with the G proteins G(o) and G(i2), using the baculovirus system. Expression levels…”
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Agonist Regulation of D sub(2) Dopamine Receptor/G Protein Interaction: EVIDENCE FOR AGONIST SELECTION OF G PROTEIN SUBTYPE by Cordeaux, Y, Nickolls, SA, Flood, LA, Graber, S G, Strange, P G

“…The D sub(2) dopamine receptor has been expressed in Sf21 insect cells together with the G proteins G sub(o) and G sub(i2), using the baculovirus system…”
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Activation of adenylyl cyclase by preincubation of rat cerebral-cortical membranes under phosphorylating conditions: role of ATP, GTP, and divalent cations by Whittemore, S R, Graber, S G, Lenox, R H, Hendley, E D, Ehrlich, Y H

“…The effects of preincubation under phosphorylating conditions on adenylyl cyclase activity were studied in preparations containing synaptic membranes from rat…”
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GTP-preferring protein phosphorylation systems in brain membranes: possible role in adenylate cyclase regulation by Ehrlich, Y H, Whittemore, S R, Lambert, R, Ellis, J, Graber, S G, Lenox, R H

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Stabilization of the GDP-bound conformation of Gialpha by a peptide derived from the G-protein regulatory motif of AGS3 by Peterson, Y K, Bernard, M L, Ma, H, Hazard, 3rd, S, Graber, S G, Lanier, S M

“…The G-protein regulatory (GPR) motif in AGS3 was recently identified as a region for protein binding to heterotrimeric G-protein alpha subunits. To define the…”
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Two Amino Acids within the alpha 4 Helix of G alpha sub(i1) Mediate Coupling with 5-Hydroxytryptamine sub(1B) Receptors by Bae, H, Cabrera-Vera, T M, Depree, K M, Graber, S G, Hamm, HE

“…We previously reported that residues 299-318 in G alpha sub(i1) participate in the selective interaction between G alpha sub(i1) and the 5-hydroxytryptamine…”
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Two amino acids within the alpha4 helix of Galphai1 mediate coupling with 5-hydroxytryptamine1B receptors by Bae, H, Cabrera-Vera, T M, Depree, K M, Graber, S G, Hamm, H E

“…We previously reported that residues 299-318 in Galphai1 participate in the selective interaction between Galphai1 and the 5-hydroxytryptamine1B (5-HT1B)…”
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Protein phosphorylation in the regulation and adaptation of receptor function by Ehrlich, Y H, Whittemore, S R, Garfield, M K, Graber, S G, Lenox, R H

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Closely Related G-protein-coupled Receptors Use Multiple and Distinct Domains on G-protein α-Subunits for Selective Coupling by Slessareva, Janna E., Ma, Hongzheng, Depree, Karyn M., Flood, Lori A., Bae, Hyunsu, Cabrera-Vera, Theresa M., Hamm, Heidi E., Graber, Stephen G.

“…The molecular basis of selectivity in G-protein receptor coupling has been explored by comparing the abilities of G-protein heterotrimers containing chimeric…”
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Reconstitution of bovine A1 adenosine receptors and G proteins in phospholipid vesicles: betagamma-subunit composition influences guanine nucleotide exchange and agonist binding by Figler, R A, Lindorfer, M A, Graber, S G, Garrison, J C, Linden, J

“…We have studied the interactions of purified A1 adenosine receptors and G proteins reconstituted into phospholipid vesicles to investigate how the betagamma…”
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