Cross-amplification and characterization of 13 tetranucleotide microsatellites in multiple species of Neotropical canids
We tested 47 tetranucleotide microsatellite loci developed for the domestic dog in four species of Neotropical canids, aiming to produce a standardized set that could be successfully used even in noninvasive samples across this group. We identified 13 suitable loci, nine of which constitute a standa...
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Published in: | Molecular ecology resources Vol. 8; no. 4; pp. 898 - 900 |
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Main Authors: | , , , , , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
Oxford, UK
Oxford, UK : Blackwell Publishing Ltd
01-07-2008
Blackwell Publishing Ltd |
Subjects: | |
Online Access: | Get full text |
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Summary: | We tested 47 tetranucleotide microsatellite loci developed for the domestic dog in four species of Neotropical canids, aiming to produce a standardized set that could be successfully used even in noninvasive samples across this group. We identified 13 suitable loci, nine of which constitute a standardized set for all species. Considering only the ideal panel of nine loci, the mean expected heterozygosity (averaged across species) per locus ranged from 0.58 to 0.92 (overall mean 0.76), and the maximum probability of identity value was 1.3 x 10⁻⁹. This set of loci has a great potential for application in evolutionary, ecological and conservation studies. |
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Bibliography: | http://dx.doi.org/10.1111/j.1755-0998.2008.02103.x ark:/67375/WNG-ZR7SP1VW-1 istex:C20C31B12418062EF1E59CB47B259EE50DE44BCE ArticleID:MEN2103 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1755-0998 1755-098X 1755-0998 |
DOI: | 10.1111/j.1755-0998.2008.02103.x |