Cross-amplification and characterization of 13 tetranucleotide microsatellites in multiple species of Neotropical canids

We tested 47 tetranucleotide microsatellite loci developed for the domestic dog in four species of Neotropical canids, aiming to produce a standardized set that could be successfully used even in noninvasive samples across this group. We identified 13 suitable loci, nine of which constitute a standa...

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Published in:Molecular ecology resources Vol. 8; no. 4; pp. 898 - 900
Main Authors: DA FONTOURA-RODRIGUES, MANOEL L, LIMA-ROSA, CARLOS ANDRÉ V, TCHAICKA, LIGIA, VALDEZ, FERNANDA P, RODRIGUES, FLÁVIO H.G, DE PAULA, ROGÉRIO C, GOUGH, MICHAEL P, JOHNSON, WARREN E, BONATTO, SANDRO L, EIZIRIK, EDUARDO
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-07-2008
Blackwell Publishing Ltd
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Summary:We tested 47 tetranucleotide microsatellite loci developed for the domestic dog in four species of Neotropical canids, aiming to produce a standardized set that could be successfully used even in noninvasive samples across this group. We identified 13 suitable loci, nine of which constitute a standardized set for all species. Considering only the ideal panel of nine loci, the mean expected heterozygosity (averaged across species) per locus ranged from 0.58 to 0.92 (overall mean 0.76), and the maximum probability of identity value was 1.3 x 10⁻⁹. This set of loci has a great potential for application in evolutionary, ecological and conservation studies.
Bibliography:http://dx.doi.org/10.1111/j.1755-0998.2008.02103.x
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content type line 23
ISSN:1755-0998
1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2008.02103.x