Intra-individual variation of glucose, specific insulin and proinsulin concentrations measured by two oral glucose tolerance tests in a general Caucasian population : the Hoorn Study

We studied the intra-individual variation in plasma glucose, specific serum insulin and serum proinsulin concentrations, measured by two 75-g oral glucose tolerance tests in an age, sex, and glucose tolerance stratified random sample from a 50-74-year-old Caucasian population without a history of di...

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Bibliographic Details
Published in:	Diabetologia Vol. 39; no. 3; pp. 298 - 305
Main Authors:	MOOY, J. M, GOOTHENHUIS, P. A, DE VRIES, H, KOSTENSE, P. J, POPP-SNIJDERS, C, BOUTER, L. M, HEINE, R. J
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-03-1996
Subjects:	Anthropometry Biological and medical sciences Blood Glucose - metabolism Blood Pressure Body Mass Index Diabetes Mellitus - blood Diabetes Mellitus - physiopathology Endocrinology European Continental Ancestry Group Glucose Intolerance - blood Glucose Intolerance - physiopathology Glucose Tolerance Test Glycated Hemoglobin A - analysis Humans Insulin - blood Investigative techniques, diagnostic techniques (general aspects) Medical sciences Netherlands Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Proinsulin - blood Reference Values Reproducibility of Results Human Proinsulin Pancreatic hormone Intraindividual comparison Reproducibility Oral administration Glucose tolerance test Glucose Caucasoid Prohormone Insulin
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Summary:	We studied the intra-individual variation in plasma glucose, specific serum insulin and serum proinsulin concentrations, measured by two 75-g oral glucose tolerance tests in an age, sex, and glucose tolerance stratified random sample from a 50-74-year-old Caucasian population without a history of diabetes mellitus. The intra-individual variation was assessed by the standard deviation of the test-retest differences (SDdif). For subjects with normal (n = 246), impaired glucose tolerance (n = 198), and newly detected diabetes (n = 80) classified at the first test, the following (SDdif/median level of individual average scores) were found: fasting glucose: 0.4/5.4, 0.5/5.9 and 0.7/7.2 mmol/l; 2-h glucose: 1.3/5.6, 1.8/8.5 and 2.3/12.8 mmol/l; fasting insulin: 23/76, 32/89 and 30/116 pmol/l; 2-h insulin: 190/303, 278/553 and 304/626 pmol/l; fasting proinsulin: 4/8, 6/13 and 9/18 pmol/l; 2-h proinsulin: 19/49, 23/84 and 33/90 pmol/l, respectively. In both glucose, proinsulin and insulin concentrations the total intra-individual variation was predominantly determined by biological variation, whereas analytical variation made only a minor contribution. The SDdif can easily be interpreted, as 95% of the random test-retest differences will be less than 2.SDdif, or in terms of percentage, less than (2.SDdif/median level of individual average score) 100. Therefore, for subjects with normal glucose tolerance, 95% of the random test-retest differences will be less than 15% (fasting glucose), 46% (2-h glucose), 61% (fasting insulin), 125% (2-h insulin), 100% (fasting proinsulin) and 78% (2-h proinsulin) of the median value of the individual average scores. No substantial independent association of either age, gender or obesity with the intra-individual variation in glucose, proinsulin, or insulin concentrations was found.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0012-186X 1432-0428
DOI:	10.1007/BF00418345