Search Results - "GLAESER, R M"

Specimen Behavior in the Electron Beam by Glaeser, R M

“…It has long been known that cryo-EM specimens are severely damaged by a level of electron exposure that is much lower than what is needed to obtain…”
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Design of an electron microscope phase plate using a focused continuous-wave laser by Müller, H, Jin, Jian, Danev, R, Spence, J, Padmore, H, Glaeser, R M

“…We propose a Zernike phase contrast electron microscope that uses an intense laser focus to convert a phase image into a visible image. We present the…”
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Current outcomes when optimizing ‘standard’ sample preparation for single‐particle cryo‐EM by CARRAGHER, B., CHENG, Y., FROST, A., GLAESER, R.M., LANDER, G.C., NOGALES, E., WANG, H.‐W.

“…Summary Although high‐resolution single‐particle cryo‐electron microscopy (cryo‐EM) is now producing a rapid stream of breakthroughs in structural biology, it…”
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Near-concentric Fabry-Pérot cavity for continuous-wave laser control of electron waves by Schwartz, O, Axelrod, J J, Tuthill, D R, Haslinger, P, Ophus, C, Glaeser, R M, Müller, H

“…Manipulating free-space electron wave functions with laser fields can bring about new electron-optical elements for transmission electron microscopy (TEM). In…”
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Images of paraffin monolayer crystals with perfect contrast: Minimization of beam-induced specimen motion by Glaeser, R.M., McMullan, G., Faruqi, A.R., Henderson, R.

“…Quantitative analysis of electron microscope images of organic and biological two-dimensional crystals has previously shown that the absolute contrast reached…”
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Accurate modeling of single-particle cryo-EM images quantitates the benefits expected from using Zernike phase contrast by Hall, R.J., Nogales, E., Glaeser, R.M.

“…The use of a Zernike-type phase plate in biologic cryo-electron microscopy allows the imaging, without using defocus, of what are predominantly phase objects…”
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Direct Evidence for Modified Solvent Structure within the Hydration Shell of a Hydrophobic Amino Acid by Pertsemlidis, Alexander, Saxena, Anand M., Soper, Alan K., Head-Gordon, Teresa, Glaeser, Robert M.

“…Neutron scattering experiments are used to determine scattering profiles for aqueous solutions of hydrophobic and hydrophilic amino acid analogs. Solutions of…”
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Three-dimensional diffractive imaging for crystalline monolayers with one-dimensional compact support by Spence, J.C.H., Weierstall, U., Fricke, T.T., Glaeser, R.M., Downing, K.H.

“…The use of a compact support constraint along the beam direction is considered as a solution to the phase problem for diffraction by two-dimensional protein…”
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Review: Electron Crystallography: Present Excitement, a Nod to the Past, Anticipating the Future by Glaeser, Robert M.

“…From a modest beginning with negatively stained samples of the helical T4 bacteriophage tail, electron crystallography has emerged as a powerful tool in…”
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High-resolution electron crystallography of protein molecules by Glaeser, R M, Downing, K H

“…Electron diffraction data and high-resolution images can now be used to obtain accurate, three-dimensional density maps of biological macromolecules. These…”
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Review: Automatic Particle Detection in Electron Microscopy by Nicholson, William V., Glaeser, Robert M.

“…Advances in cryoEM and single-particle reconstruction have led to results at increasingly high resolutions. However, to sustain continuing improvements in…”
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Structural comparison of native and deoxycholate-treated purple membrane by Glaeser, R.M., Jubb, J.S., Henderson, R.

“…Lipid-depleted purple membrane prepared by extraction with deoxycholate has been compared with the native structure. X-ray and electron diffraction photographs…”
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Specimen flatness of thin crystalline arrays: influence of the substrate by Glaeser, R M

“…The extreme degree of specimen flatness (i.e. planarity) required for high-resolution electron diffraction and electron microscopy at high tilt angles cannot…”
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Structure of an Early Intermediate in the M-State Phase of the Bacteriorhodopsin Photocycle by Facciotti, Marc T., Rouhani, Shahab, Burkard, Fredrick T., Betancourt, Felicia M., Downing, Kenneth H., Rose, Robert B., McDermott, Gerry, Glaeser, Robert M.

“…The structure of an early M-intermediate of the wild-type bacteriorhodopsin photocycle formed by actinic illumination at 230 K has been determined by x-ray…”
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Probing Toward Atomic Resolution in Molecular Topography by Glaeser, Robert M.

“…A study in which the atomic force microscopy of biological macromolecules was examined is praised for its sophistication of the quantitative comparison made…”
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Interfacial energies and surface-tension forces involved in the preparation of thin, flat crystals of biological macromolecules for high-resolution electron microscopy by Glaeser, R M, Zilker, A, Radermacher, M, Gaub, H E, Hartmann, T, Baumeister, W

“…It is generally agreed that surface-tension forces and the direct interaction between the specimen and either the air-water interface or the water-substrate…”
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Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediate by Rouhani, Shahab, Cartailler, Jean-Philippe, Facciotti, Marc T, Walian, Peter, Needleman, Richard, Lanyi, Janos K, Glaeser, Robert M, Luecke, Hartmut

“…Crystal structures are reported for the D85S and D85S/F219L mutants of the light-driven proton/hydroxyl-pump bacteriorhodopsin. These mutants crystallize in…”
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Electron diffraction analysis of the M412 intermediate of bacteriorhodopsin by Glaeser, R.M., Baldwin, J., Ceska, T.A., Henderson, R.

“…High resolution electron diffraction data have been recorded for glucose-embedded purple membrane specimens in which bacteriorhodopsin (bR) has been trapped by…”
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Glucose alone does not completely hydrate bacteriorhodopsin in glucose-embedded purple membrane by Perkins, G A, Burkard, F, Liu, E, Glaeser, R M

“…Glucose embedding is a simple and highly effective method for preparing biological macromolecules for high-resolution electron microscopy. The investigation of…”
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Assessment of resolution in biological electron crystallography by Glaeser, R M, Downing, K H

“…The resolution of images or density maps produced by electron microscopy and electron crystallography can be objectively defined in terms of the spatial…”
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