A Rapid and Efficient Method for Plasmid Transformation of Klebsiella pneumoniae and Escherichia coli

A Rapid and Efficient Method for Plasmid Transformation of Klebsiella pneumoniae and Escherichia coli

AFRC Unit of Nitrogen Fixation, University of Sussex, Brighton BNl 9RQ, UK ABSTRACT SUMMARY: A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae M5al and Escherichia coli K12 has been developed. The method, which uses a freeze-thaw cycle in the presence of CaCl 2 to faci...

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Bibliographic Details
Published in:Journal of general microbiology Vol. 133; no. 8; pp. 2053 - 2057
Main Authors: MERRICK, MIKE J, GIBBINS, JANE R, POSTGATE, JOHN R
Format: Journal Article
Language:English
Published: London Soc General Microbiol 01-08-1987
New York, NY Cambridge University Press
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Escherichia coli
Escherichia coli - genetics
Freezing
Fundamental and applied biological sciences. Psychology
Klebsiella pneumoniae
Klebsiella pneumoniae - genetics
Methods
Microbiology
Plasmids
Temperature
Transformation, Bacterial
Plasmid
Genetic transformation
Escherichia coli
Bacteria
Method
Escherichieae
Klebsielleae
Freezing
Klebsiella pneumoniae
Enterobacteriaceae
Genetic transfer
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Summary:AFRC Unit of Nitrogen Fixation, University of Sussex, Brighton BNl 9RQ, UK ABSTRACT SUMMARY: A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae M5al and Escherichia coli K12 has been developed. The method, which uses a freeze-thaw cycle in the presence of CaCl 2 to facilitate DNA uptake, is substantially more efficient for K. pneumoniae M5al than the conventional transformation procedure for E. coli . The simplicity and speed of the method makes it very attractive for routine transformation of K. pneumoniae M5al and E. coli K12.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0022-1287
1350-0872
1465-2080
DOI:10.1099/00221287-133-8-2053