Search Results - "GHELARDINI, P"

Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation by Di Lallo, G, Fagioli, M, Barionovi, D, Ghelardini, P, Paolozzi, L

“…1 Dipartimento di Biologia, Università "Tor Vergata", Via della Ricerca Scientifica, I-00133 Rome, Italy 2 Istituto di Biologia e Patologia Molecolari del CNR,…”
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Essential and non-essential interactions in interactome networks: the Escherichia coli division proteins FtsQ-FtsN interaction by Grenga, L., Rizzo, A., Paolozzi, L., Ghelardini, P.

“…Summary The Escherichia coli division protein FtsQ, which plays a central role in the septosome assembly, interacts with several protein partners of the…”
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Three functional subdomains of the Escherichia coli FtsQ protein are involved in its interaction with the other division proteins by D'Ulisse, V, Fagioli, M, Ghelardini, P, Paolozzi, L

“…1 Dipartimento di Biologia Università ‘Tor Vergata’, via della Ricerca Scientifica, Roma 00133, Italy 2 Laboratorio di Biologia e Patologia Molecolari del CNR…”
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FtsQ interaction mutants: a way to identify new antibacterial targets by Grenga, L., Guglielmi, G., Melino, S., Ghelardini, P., Paolozzi, L.

“…FtsQ is a highly conserved component of the divisome that plays a central role in the assembly of early and late cell division proteins. The biological…”
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Prokaryotic division interactome: setup of an assay for protein–protein interaction mutant selection by Barbati, S., Grenga, L., Luzi, G., Paolozzi, L., Ghelardini, P.

“…A method which enables selection of protein mutants impaired in their ability to interact with their normal protein partners is presented here. The method is…”
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A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli by Di Lallo, G, Castagnoli, L, Ghelardini, P, Paolozzi, L

“…Dipartimento di Biologia, Università ’Tor Vergata’, via della Ricerca Scientifica, 00133 La Romanina (Roma), Italy 1 Centro Acidi Nucleici del CNR, Roma, Italy…”
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H-NS regulation of virulence gene expression in enteroinvasive Escherichia coli harboring the virulence plasmid integrated into the host chromosome by Colonna, B, Casalino, M, Fradiani, P A, Zagaglia, C, Naitza, S, Leoni, L, Prosseda, G, Coppo, A, Ghelardini, P, Nicoletti, M

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Two-hybrid assay: construction of an Escherichia coli system to quantify homodimerization ability in vivo by Di Lallo, G, Ghelardini, P, Paolozzi, L

“…1 Dipartimento di Biologia, Università ‘Tor Vergata’ Roma, Via della Ricerca Scientifica, 00133 La Romanina (Roma), Italy 2 Centro Acidi Nucleici del CNR,…”
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Mutations arise independently of transcription in non-dividing bacteria by Barionovi, D, Ghelardini, P, Di Lallo, G, Paolozzi, L

“…It has been proposed that transcription introduces a bias into the random process of mutation. Although this hypothesis is supported by experimental data for…”
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Mu DNA reintegration upon excision: evidence for a possible involvement of nucleoid folding by Paolozzi, L, Fabozzi, G, Ghelardini, P

“…Dipartimento di Biologia, Università ‘Tor Vergata’ Roma, Italy 1 Author for correspondence: P. Ghelardini. Tel: +39 6 72594674. Fax: +39 6 2023500. e-mail:…”
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FtsZ dimerization in vivo by Di Lallo, G., Anderluzzi, D., Ghelardini, P., Paolozzi, L.

“…A hybrid assay, based on the properties of the λ repressor, was developed to detect FtsZ dimerization in Escherichia coli in vivo. A gene fusion comprising the…”
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Regulation of the bacteriophage Mu gem operon by Fabozzi, G, Paolozzi, L, Ghelardini, P

“…The gem operon of bacteriophage Mu, responsible for the complex phenomenon of phage conversion, is included in the so called "semiessential early" region of…”
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Essential and non‐essential interactions in interactome networks: the E scherichia coli division proteins FtsQ – FtsN interaction by Grenga, L., Rizzo, A., Paolozzi, L., Ghelardini, P.

“…The E scherichia coli division protein FtsQ , which plays a central role in the septosome assembly, interacts with several protein partners of the division…”
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Mu gem2ts DNA integration is not necessary for induction of synchrony of cell division in Escherichia coli K12 by Bobrowicz, P., Paolozzi, L., Ghelardini, P.

“…The gem2ts mutant of bacteriophage Mu induced synchrony of cell division on bacteria surviving infection. Induction of synchronous growth could also be…”
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Bacteriophage Mu gem2ts, which induces synchronous cell division in the infected host, is mutated in the gem operon promoter by La Valle, R, Fabozzi, G, Ghelardini, P, Paolozzi, L

“…An A to G transition in position −44 of the Mu gem promoter region confers the ability to induce synchrony of cell division in bacteria infected by this phage…”
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A case of lysogenic conversion: modification of cell phenotype by constitutive expression of the Mu gem operon by Paolozzi, L, Ghelardini, P

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The Mu gem operon: its role in gene expression, recombination and cell cycle by Ghelardini, P, La Valle, R, Paolozzi, L

“…Two genes, gemA and gemB, belong to the gem operon located in the semi-essential early region of bacteriophage Mu. The product of gemA modulates the expression…”
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Reversal of Mu gem2ts-induced mutations by Ghelardini, P., Liébart, J.C., Fabozzi, G., Tomassini, B., D'Ari, R., Paolozzi, L.

“…Mutations induced by the integration of a Mu gem2ts mutant prophage can revert at frequencies around 1 × 10 −6, more than 10 4-fold higher than that obtained…”
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Conservative Integration of Bacteriophage Mu DNA into pBR322 Plasmid by Liebart, J. C., Ghelardini, P., Paolozzi, L.

“…In order to clarify the first step in Mu integrative recombination, we have infected a bacterial strain harboring the plasmid pBR322 and isolated Mu DNA in a…”
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A novel illegitimate recombination event: precise excision and reintegration with the Mu gem mutant prophage by Ghelardini, P, Liébart, J C, Di Zenzo, G, Micheli, G, D'Ari, R, Paolozzi, L

“…The bacteriophage Mu is known to insert its DNA more or less randomly within the Escherichia coli chromosome, as do transposable elements, but unlike the…”
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