A polyprotein precursor of two mitochondrial enzymes in Neurospora crassa: gene structure and precursor processing

N-acetylglutamate kinase (AGK) and N-acetyl-gamma-glutamyl-phosphate reductase (AGPR) function as two separate mitochondrial enzymes, but are encoded by a single nuclear gene in several fungi. The Neurospora crassa arg-6 gene encoding these enzymes has been cloned and sequenced, and the enzymes resp...

Full description

Saved in:

Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 269; no. 11; pp. 8189 - 8203
Main Authors:	GESSERT, S. F, JEONG HEE KIM, NARGANG, F. E, WEISS, R. L
Format:	Journal Article
Language:	English
Published:	Bethesda, MD American Society for Biochemistry and Molecular Biology 18-03-1994
Subjects:	Aldehyde Oxidoreductases - biosynthesis Aldehyde Oxidoreductases - genetics Amino Acid Sequence Base Sequence Biological and medical sciences Biological Evolution Chromosome Mapping Chromosomes, Fungal Cloning, Molecular Conserved Sequence Cosmids enzyme precursors Enzyme Precursors - genetics Enzyme Precursors - metabolism Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology gene Gene expression genes Genes, Fungal mitochondria Mitochondria - enzymology mitochondrie mitocondria Molecular and cellular biology Molecular genetics Molecular Sequence Data neurospora Neurospora crassa Neurospora crassa - enzymology Neurospora crassa - genetics nucleotide sequence oxidoreductases oxidorreductasas oxydoreductase Phosphotransferases (Carboxyl Group Acceptor) - biosynthesis Phosphotransferases (Carboxyl Group Acceptor) - genetics Plasmids Polymorphism, Restriction Fragment Length precurseur d' enzyme precursores de enzimas Protein Biosynthesis Protein Processing, Post-Translational Restriction Mapping secuencia nucleica Sequence Homology, Amino Acid sequence nucleique Transcription, Genetic transferasas transferase transferases Transformation, Genetic Molecular processing Enzyme Transferases Primary structure Fungi Mitochondria Gene Ascomycetes Neurospora crassa Biosynthesis precursor Oxidoreductases Acetylglutamate kinase N-Acetyl-γ-glutamyl-phosphate reductase Thallophyta
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	N-acetylglutamate kinase (AGK) and N-acetyl-gamma-glutamyl-phosphate reductase (AGPR) function as two separate mitochondrial enzymes, but are encoded by a single nuclear gene in several fungi. The Neurospora crassa arg-6 gene encoding these enzymes has been cloned and sequenced, and the enzymes responsible for processing the polyprotein precursor have been identified. The 871-amino acid precursor contains a normal N-terminal mitochondrial targeting sequence, an internal connecting region (approximately 200 amino acids) upstream of the distal reductase domain, and coding regions with N-terminal amino acid sequences identical with those of purified N-acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase. Sequence comparisons of the coding regions indicate high levels of conservation between prokaryotic and fungal proteins. Regions suggesting ancestral relationships to N-acetylglutamate synthase and aspartate beta-semialdehyde dehydrogenase have been identified. Both the N-terminal targeting sequence and the connecting region contain consensus sites for cleavage by the mitochondrial processing peptidase and processing enhancing protein. In vitro processing assays with intact mitochondria, solubilized mitochondria, and purified enzymes have shown that the mitochondrial processing peptidase and processing enhancing protein cleave not only the N-terminal mitochondrial targeting sequence but also process the polyprotein precursor into the two mature enzymes.
Bibliography:	F30 F ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0021-9258 1083-351X
DOI:	10.1016/S0021-9258(17)37179-X