Search Results - "GELVIN, S. B"

Improving plant genetic engineering by manipulating the host by Gelvin, Stanton B

“…Agrobacterium-mediated transformation is a major technique for the genetic engineering of plants. However, there are many economically important crop and tree…”
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Integration of T‐DNA binary vector ‘backbone’ sequences into the tobacco genome: evidence for multiple complex patterns of integration by Kononov, Maria E., Bassuner, Burgund, Gelvin, Stanton B.

“…During the process of crown gall tumorigenesis, Agrobacterium tumefaciens transfers part of the tumor‐inducing (Ti) plasmid, the T‐DNA, to a plant cell where…”
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Early transcription of Agrobacterium T-DNA genes in tobacco and maize by Narasinhulu, S.B. (Colorado State University, Ft. Collins, CO.), Deng, X.B, Sarria, R, Gelvin, S.B

“…We developed a sensitive procedure to investigate the kinetics of transcription of an Agrobacterium tumefaciens transferred (T)-DNA-encoded beta-glucuronidase…”
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Differences in susceptibility of arabidopsis ecotypes to crown gall disease may result from a deficiency in T-DNA integration by Nam, J.S. (Purdue University, West Lafayette, IN.), Matthysse, A.G, Gelvin, S.B

“…We show that among ecotypes of Arabidopsis, there is considerable variation in their susceptibility to crown gall disease. Differences in susceptibility are…”
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Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells by Yusibov, V.M, Steck, T.R, Gupta, V, Gelvin, S.B

“…During the inception of crown gall tumorigenesis, the transferred DNA (T-DNA) is processed from the Ti (tumor inducing) plasmid of Agrobacterium tumefaciens…”
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AGROBACTERIUM AND PLANT GENES INVOLVED IN T-DNA TRANSFER AND INTEGRATION by Gelvin, Stanton B.

“…The phytopathogenic bacterium Agrobacterium tumefaciens genetically transforms plants by transferring a portion of the resident Ti-plasmid, the T-DNA, to the…”
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Role of the Agrobacterium tumefaciens VirD2 protein in T-DNA transfer and integration by Mysore, K.S, Bassuner, B, Deng, X, Darbinian, N.S, Motchoulski, A, Ream, W, Gelvin, S.B

“…VirD2 is one of the key Agrobacterium tumefaciens proteins involved in T-DNA processing and transfer. In addition to its endonuclease domain, VirD2 contains a…”
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Mutation of the miaA gene of Agrobacterium tumefaciens results in reduced vir gene expression by Gray, J, Wang, J, Gelvin, S B

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Arabidopsis ecotypes and mutants that are recalcitrant to Agrobacterium root transformation are susceptible to germ‐line transformation by Mysore, Kirankumar S., Kumar, C. T. Ranjith, Gelvin, Stanton B.

“…Summary Germ‐line transformation (vacuum infiltration) is frequently used to transform Arabidopsis thaliana using Agrobacterium tumefaciens. We have recently…”
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Transfer RNA is the source of extracellular isopentenyladenine in a Ti-plasmidless strain of Agrobacterium tumefaciens by Gray, J. (University of Missouri, Columbia, MO.), Gelvin, S.B, Meilan, R, Morris, R.O

“…Even in the absence of the classical Ti plasmid-encoded cytokinin biosynthetic genes ipt and tzs, Agrobacterium tumefaciens strains still release significant…”
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A cytoplasmic male sterility-associated mitochondrial protein causes pollen disruption in transgenic tobacco by He, S.C. (Cornell University, Ithaca, NY.), Abad, A.R, Gelvin, S.B, Mackenzie, S.A

“…In higher plants, dominant mitochondrial mutations are associated with pollen sterility. This phenomenon is known as cytoplasmic male sterility (CMS). It is…”
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Transfer of T‐DNA and Vir proteins to plant cells by Agrobacterium tumefaciens induces expression of host genes involved in mediating transformation and suppresses host defense gene expression by Jiang, Hongmei, Doerge, R.W., Gelvin, Stanton B.

“…Summary Agrobacterium tumefaciens is a plant pathogen that incites crown gall tumors by transferring to and expressing a portion of a resident plasmid in plant…”
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Virulence genes, borders, and overdrive generate single-stranded T-DNA molecules from the A6 Ti plasmid of Agrobacterium tumefaciens by Veluthambi, K, Ream, W, Gelvin, S B

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Opines stimulate induction of the vir genes of the Agrobacterium tumefaciens Ti plasmid by Veluthambi, K. (Madurai Kamaraj University, Madurai, India), Krishnan, M, Gould, J.H, Smith, R.H, Gelvin, S.B

“…Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of…”
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Agrobacterium tumefaciens transformation of the radiation hypersensitive Arabidopsis thaliana mutants uvh1 and rad5 by Nam, J.S, Mysore, K.S, Gelvin, S.B

“…The Arabidopsis thaliana mutants uvh1 and rad5, originally identified as radiation hypersensitive, were reported to be deficient in T-DNA integration based on…”
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Formation of a putative relaxation intermediate during T-DNA processing directed by the Agrobacterium tumefaciens VirD1,D2 endonuclease by Filichkin, S A, Gelvin, S B

“…During the initial stages of crown gall tumorigenesis, the T-DNA region of the Agrobacterium tumefaciens Ti-plasmid is processed, resulting in the production…”
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Novel constructions to enable the integration of genes into the Agrobacterium tumefaciens C58 chromosome by LEE, Lan-Ying, HUMARA, Jaime M, GELVIN, Stanton B

“…We constructed several versatile sets of vectors that can be used to introduce any gene into the pgl/picA locus of the Agrobacterium tumefaciens C58 chromosome…”
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Crown gall disease and hairy root disease. A sledgehammer and a tackhammer by Gelvin, S.B. (Purdue University, West Lafayette, IN)

“…The neoplastic diseases crown gall and hairy root are incited by the phytopathogenic bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes,…”
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Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens by Jayaswal, R.K, Veluthambi, K, Gelvin, S.B, Slightom, J.L

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Effect of dimethyl sulfoxide concentration on specificity of primer matching in PCR by Filichkin, S A, Gelvin, S B

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