Search Results - "GAUCHER, A"

Evolution of bacterial steroid biosynthesis and its impact on eukaryogenesis by Hoshino, Yosuke, Gaucher, Eric A.

“…Steroids are components of the eukaryotic cellular membrane and have indispensable roles in the process of eukaryotic endocytosis by regulating membrane…”
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Evolutionary history and metabolic insights of ancient mammalian uricases by Kratzer, James T., Lanaspa, Miguel A., Murphy, Michael N., Cicerchi, Christina, Graves, Christina L., Tipton, Peter A., Ortlund, Eric A., Johnson, Richard J., Gaucher, Eric A.

“…Uricase is an enzyme involved in purine catabolism and is found in all three domains of life. Curiously, uricase is not functional in some organisms despite…”
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Coevolution of URAT1 and Uricase during Primate Evolution: Implications for Serum Urate Homeostasis and Gout by Tan, Philip K, Farrar, Jennifer E, Gaucher, Eric A, Miner, Jeffrey N

“…Uric acid is the highly insoluble end-product of purine metabolism in humans. Serum levels exceeding the solubility threshold can trigger formation of urate…”
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Palaeotemperature trend for Precambrian life inferred from resurrected proteins by Gaucher, Eric A, Govindarajan, Sridhar, Ganesh, Omjoy K

“…Biosignatures and structures in the geological record indicate that microbial life has inhabited Earth for the past 3.5 billion years or so. Research in the…”
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Enhancing the pharmaceutical properties of protein drugs by ancestral sequence reconstruction by Zakas, Philip M, Brown, Harrison C, Knight, Kristopher, Meeks, Shannon L, Spencer, H Trent, Gaucher, Eric A, Doering, Christopher B

“…The pharmaceutical properties of coagulation factor VIII are improved by reconstructing the protein's ancestral sequence. Optimization of a protein's…”
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Conservation of Protein Structure over Four Billion Years by Ingles-Prieto, Alvaro, Ibarra-Molero, Beatriz, Delgado-Delgado, Asuncion, Perez-Jimenez, Raul, Fernandez, Julio M., Gaucher, Eric A., Sanchez-Ruiz, Jose M., Gavira, Jose A.

“…Little is known about the evolution of protein structures and the degree of protein structure conservation over planetary time scales. Here, we report the…”
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Accuracy of respiratory rate monitoring using a non-invasive acoustic method after general anaesthesia by Mimoz, O., Benard, T., Gaucher, A., Frasca, D., Debaene, B.

“…Respiratory rate should be monitored continuously in the post-anaesthesia care unit (PACU) to avoid any delay in the detection of respiratory depression…”
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Uric acid‐dependent inhibition of AMP kinase induces hepatic glucose production in diabetes and starvation: evolutionary implications of the uricase loss in hominids by Cicerchi, Christina, Li, Nanxing, Kratzer, James, Garcia, Gabriela, Roncal‐Jimenez, Carlos A., Tanabe, Katsuyuki, Hunter, Brandi, Rivard, Christopher J., Sautin, Yuri Y., Gaucher, Eric A., Johnson, Richard J., Lanaspa, Miguel A.

“…Reduced AMP kinase (AMPK) activity has been shown to play a key deleterious role in increased hepatic gluconeogenesis in diabetes, but the mechanism whereby…”
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An experimental phylogeny to benchmark ancestral sequence reconstruction by Randall, Ryan N., Radford, Caelan E., Roof, Kelsey A., Natarajan, Divya K., Gaucher, Eric A.

“…Ancestral sequence reconstruction (ASR) is a still-burgeoning method that has revealed many key mechanisms of molecular evolution. One criticism of the…”
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Hydrophobic environment is a key factor for the stability of thermophilic proteins by Gromiha, M. Michael, Pathak, Manish C., Saraboji, Kadhirvel, Ortlund, Eric A., Gaucher, Eric A.

“…The stability of thermophilic proteins has been viewed from different perspectives and there is yet no unified principle to understand this stability. It would…”
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De novo active sites for resurrected Precambrian enzymes by Risso, Valeria A., Martinez-Rodriguez, Sergio, Candel, Adela M., Krüger, Dennis M., Pantoja-Uceda, David, Ortega-Muñoz, Mariano, Santoyo-Gonzalez, Francisco, Gaucher, Eric A., Kamerlin, Shina C. L., Bruix, Marta, Gavira, Jose A., Sanchez-Ruiz, Jose M.

“…Protein engineering studies often suggest the emergence of completely new enzyme functionalities to be highly improbable. However, enzymes likely catalysed…”
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Accuracy of respiratory rate monitoring by capnometry using the Capnomask® in extubated patients receiving supplemental oxygen after surgery by Gaucher, A., Frasca, D., Mimoz, O., Debaene, B.

“…Respiratory monitoring is standard after anaesthesia and surgery. Abnormal respiratory rate is a sensitive indicator of respiratory problems, even in patients…”
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Inferring the palaeoenvironment of ancient bacteria on the basis of resurrected proteins by Gaucher, Eric A., Thomson, J. Michael, Burgan, Michelle F., Benner, Steven A.

“…Features of the physical environment surrounding an ancestral organism can be inferred by reconstructing sequences of ancient proteins made by those organisms,…”
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Heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase by Gamiz-Arco, Gloria, Gutierrez-Rus, Luis I., Risso, Valeria A., Ibarra-Molero, Beatriz, Hoshino, Yosuke, Petrović, Dušan, Justicia, Jose, Cuerva, Juan Manuel, Romero-Rivera, Adrian, Seelig, Burckhard, Gavira, Jose A., Kamerlin, Shina C. L., Gaucher, Eric A., Sanchez-Ruiz, Jose M.

“…Glycosidases are phylogenetically widely distributed enzymes that are crucial for the cleavage of glycosidic bonds. Here, we present the exceptional properties…”
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Reconstitution of translation from Thermus thermophilus reveals a minimal set of components sufficient for protein synthesis at high temperatures and functional conservation of modern and ancient translation components by Zhou, Ying, Asahara, Haruichi, Gaucher, Eric A, Chong, Shaorong

“…Thermus thermophilus is a thermophilic model organism distantly related to the mesophilic model organism E. coli. We reconstituted protein translation of…”
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The molecular evolution of the p120-catenin subfamily and its functional associations by Carnahan, Robert H, Rokas, Antonis, Gaucher, Eric A, Reynolds, Albert B

“…p120-catenin (p120) is the prototypical member of a subclass of armadillo-related proteins that includes δ-catenin/NPRAP, ARVCF, p0071, and the more distantly…”
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Structural and Dynamics Comparison of Thermostability in Ancient, Modern, and Consensus Elongation Factor Tus by Okafor, C. Denise, Pathak, Manish C., Fagan, Crystal E., Bauer, Nicholas C., Cole, Megan F., Gaucher, Eric A., Ortlund, Eric A.

“…Rationally engineering thermostability in proteins would create enzymes and receptors that function under harsh industrial applications. Several sequence-based…”
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Phenotypic comparisons of consensus variants versus laboratory resurrections of Precambrian proteins by Risso, Valeria A., Gavira, Jose A., Gaucher, Eric A., Sanchez-Ruiz, Jose M.

“…ABSTRACT Consensus‐sequence engineering has generated protein variants with enhanced stability, and sometimes, with modulated biological function. Consensus…”
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Secondary structure and domain architecture of the 23S and 5S rRNAs by Petrov, Anton S, Bernier, Chad R, Hershkovits, Eli, Xue, Yuzhen, Waterbury, Chris C, Hsiao, Chiaolong, Stepanov, Victor G, Gaucher, Eric A, Grover, Martha A, Harvey, Stephen C, Hud, Nicholas V, Wartell, Roger M, Fox, George E, Williams, Loren Dean

“…We present a de novo re-determination of the secondary (2°) structure and domain architecture of the 23S and 5S rRNAs, using 3D structures, determined by X-ray…”
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CRISPR-Cas9-mediated reactivation of the uricase pseudogene in human cells prevents acute hyperuricemia by de Lima Balico, Lais, Gaucher, Eric A.

“…The utility of CRISPR-Cas9 to repair or reverse diseased states that arise from recent genetic mutations in the human genome is now widely appreciated. The use…”
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