Filtration, adsorption and immunodetection of virus using polyelectrolyte multilayer-modified paper

Filtration, adsorption and immunodetection of virus using polyelectrolyte multilayer-modified paper

[Display omitted] ► A rapid paper-based immunodetection method for viruses in water has been developed. ► Virus adsorption to the paper was significantly enhanced by adsorbing polyelectrolyte multilayers (PEMs) onto filter papers prior to filtration of virus suspensions. ► The use of PEMs in combina...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces Vol. 101; pp. 205 - 209
Main Authors: Larsson, Per A., G. Puttaswamaiah, Sushma, Ly, Christine, Vanerek, Alois, Christopher Hall, J., Drolet, François
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-01-2013
Subjects:
Acrylic Resins - chemistry
Adsorption
Bacteriophage M13 - chemistry
Bacteriophage M13 - isolation & purification
Benzidines - chemistry
Cellulose
Electrochemistry
Electrolytes - chemistry
ELISA
Enzyme-Linked Immunosorbent Assay
Filtration
Horseradish Peroxidase - chemistry
Hydrogen-Ion Concentration
Immunodetection
Limit of Detection
M13
Paper-ELISA
Polyamines - chemistry
Polyelectrolyte multilayer
Polymers
Virus detection
Viruses - chemistry
Virus detection
Paper-ELISA
Polyelectrolyte multilayer
M13
Immunodetection
ELISA
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Summary:[Display omitted] ► A rapid paper-based immunodetection method for viruses in water has been developed. ► Virus adsorption to the paper was significantly enhanced by adsorbing polyelectrolyte multilayers (PEMs) onto filter papers prior to filtration of virus suspensions. ► The use of PEMs in combination with casein blocking reduced non-specific binding to a minimum. ► The assay performs as well as, or even better than, conventional ELISA based on the same detection system. The lowest concentration detected being 5×104pfu/ml. A new method for detection of viruses has been developed. The entire assay can be performed within 2h, and consists of a polyelectrolyte-multilayer-modified cellulosic filter paper combined with immunodetection. The M13 bacteriophage was used as a model virus. A visual colour-based detection system, anti-M13 horseradish peroxidase (HRP) conjugate and 3,3′,5,5′-tetramethylbenzidine (TMB), was selected to allow semi-quantitative assessment by human eye, or quantitative assessment using a digital scanner. By filtering a volume of 0.50ml, it was possible to visually detect a concentration of 106pfu/ml. The detection limit was improved to 5×104pfu/ml by increasing the volume of the sample to 100ml. For comparison, it was only possible to detect a concentration of 107pfu/ml using conventional sandwich enzyme-linked immunosorbent assay (ELISA) with the same detection system.
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ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2012.06.023