Search Results - "Günther Sillero, María A."

Synthesis of bisphosphonate derivatives of ATP by T4 DNA ligase, ubiquitin activating enzyme (E1) and other ligases by Günther Sillero, María A., de Diego, Anabel, Pérez-Zúñiga, Francisco J., Sillero, Antonio

“…T4 DNA ligase and the ubiquitin activating enzyme (E1), catalyze the synthesis of ATP β,γ-bisphosphonate derivatives. Concerning T4 DNA ligase: (i) etidronate…”
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Bisphosphonates activate the 5-fluorouracil/uracil phosphoribosyltransferase activity present in Saccharomyces cerevisiae cell extracts Implications for tumor treatments by PEREZ-ZUNIGA, Francisco J, GÜNTHER SILLERO, Maria A, SILLERO, Antonio

“…Most of the effects described for bisphosphonates (pC(R1)(R2)p) are related, directly or indirectly with a pyrophosphate moiety. Bisphosphonates are (i)…”
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Micromolar HgCl2 concentrations transitorily duplicate the ATP level in Saccharomyces cerevisiae cells by Silles, Eduardo, Osorio, Hugo, Maia, Rita, Günther Sillero, María A., Sillero, Antonio

“…Low concentrations of HgCl2 elicited, in Saccharomyces cerevisiae, a transitory increase in the ATP level followed by a decrease of its concentration, until…”
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Dehydroluciferyl‐AMP is the main intermediate in the luciferin dependent synthesis of Ap4A catalyzed by firefly luciferase by Fontes, Rui, Ortiz, Begoña, de Diego, Anabel, Sillero, Antonio, Günther Sillero, Marı́a A.

“…It was previously assumed that E·LH2‐AMP was the intermediate complex in the synthesis of Ap4A catalyzed by firefly luciferase (EC 1.13.12.7), when luciferin…”
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Polyphosphates strongly inhibit the tRNA dependent synthesis of poly(A) catalyzed by poly(A) polymerase from Saccharomyces cerevisiae by Günther Sillero, Marı́a A, de Diego, Anabel, Silles, Eduardo, Osorio, Hugo, Sillero, Antonio

“…Polyphosphates of different chain lengths (P 3, P 4, P 15, P 35), (1 μM) inhibited 10, 60, 90 and 100%, respectively, the primer (tRNA) dependent synthesis of…”
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Acyl-CoA synthetase catalyzes the synthesis of diadenosine hexaphosphate (Ap 6A) by Fontes, Rui, Günther Sillero, María A, Sillero, Antonio

“…The synthesis of diadenosine hexaphosphate (Ap 6A), a potent vasoconstrictor, is catalyzed by acyl-CoA synthetase from Pseudomonas fragi. In a first step AMP…”
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Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase by Sillero, María A. Günther, de Diego, Anabel, Osorio, Hugo, Sillero, Antonio

“…Novel properties of the primer independent synthesis of poly(A), catalyzed by the yeast poly(A) polymerase are presented. The commercial enzyme from yeast, in…”
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Acyl Coenzyme A Synthetase from Pseudomonas fragi Catalyzes the Synthesis of Adenosine 5′-Polyphosphates and Dinucleoside Polyphosphates by Fontes, R, Sillero, M A, Sillero, A

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Synthesis of ATP derivatives of compounds of the mevalonate pathway (isopentenyl di- and triphosphate; geranyl di- and triphosphate, farnesyl di- and triphosphate, and dimethylallyl diphosphate) catalyzed by T4 RNA ligase, T4 DNA ligase and other ligases Potential relationship with the effect of bisphosphonates on osteoclasts by GÜNTHER SILLERO, Maria A, DE DIEGO, Anabel, TAVARES, Janeth E. F, CATANHO DA SILVA, Joana A. D, PEREZ-ZUNIGA, Francisco J, SILLERO, Antonio

“…Compounds of the mevalonate pathway containing a terminal di- or triphosphate (mev-PP or mev-PPP) were tested as substrates of several enzyme ligases (T4 RNA…”
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Synthesis of (di)nucleoside polyphosphates by the ubiquitin activating enzyme E1 by Günther Sillero, Maria A., de Diego, Anabel, Silles, Eduardo, Sillero, Antonio

“…Previous work from this laboratory had shown that ligases may catalyze the synthesis of (di)nucleoside polyphosphates. Here, we show that one of the enzymes of…”
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Synthesis of FUDP-N-acetylglucosamine and FUDP-glucose in Saccharomyces cerevisiae cells treated with 5-fluorouracil by Günther Sillero, María A, Pérez-Zúñiga, Francisco, Gomes, Joana, de Carvalho, Ana Isabel, Martins, Susana, Silles, Eduardo, Sillero, Antonio

“…Saccharomyces cerevisiae cells (strain W303-1A) treated with 5-fluorouracil and grown in 2% (fermentative conditions) or in 0.1 % glucose (oxidative…”
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Influence of chronological aging on the survival and nucleotide content of Saccharomyces cerevisiae cells grown in different conditions: occurrence of a high concentration of UDP- N-acetylglucosamine in stationary cells grown in 2% glucose by Osório, Hugo, Silles, Eduardo, Maia, Rita, Peleteiro, Bárbara, Moradas-Ferreira, Pedro, Günther Sillero, María A., Sillero, Antonio

“…Saccharomyces cerevisiae cells (strain W303) grown in a minimal medium (containing 2% or 0.1% glucose) until exponential or stationary phase, were subjected to…”
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In Saccharomyces cerevisiae, the effect of H2O2 on ATP, but not on glyceraldehyde-3-phosphate dehydrogenase, depends on the glucose concentration by Osorio, H, Moradas-Ferreira, P, Gunther Sillero, M.A, Sillero, A

“…As has been previously shown, Saccharomyces cerevisiae grown in 2% or 0.025% glucose uses this carbohydrate by the fermentative or oxidative pathways,…”
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Synthesis of bisphosphonate derivatives of ATP by T4 RNA ligase by Sillero, María A. Günther, de Diego, Anabel, Silles, Eduardo, Pérez-Zúñiga, Francisco, Sillero, Antonio

“…T4 RNA ligase catalyzes the synthesis of ATP β,γ-bisphosphonate analogues, using the following substrates with the relative velocity rates indicated between…”
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H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae. An experimental and theoretical approach by Osorio, Hugo, Carvalho, Elisabete, del Valle, Mercedes, Günther Sillero, María A, Moradas-Ferreira, Pedro, Sillero, Antonio

“…When Saccharomyces cerevisiae cells, grown in galactose, glucose or mannose, were treated with 1.5 mm hydrogen peroxide (H2O2) for 30 min, an important…”
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Determination of enzymatic activities and metabolites in microliter sample size by Sillero, Maria A. Günther, Diego, Anabel de, Rodríguez-Tarduchy, Gemma, Sillero, Antonio

“…A new generation of spectrophotometers able to measure a wide range of absorbance in microliter aliquots is currently used for the determination of DNA, RNA,…”
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Time course of luciferyl adenylate synthesis in the firefly luciferase reaction by Dukhovich, Alexey, Sillero, Antonio, Günther Sillero, Maria A

“…The time course of luciferyl adenylate formation in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been followed. The properties of luciferyl…”
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Poly(A) polymerase from Escherichia coli adenylylates the 3′‐hydroxyl residue of nucleosides, nucleoside 5′‐phosphates and nucleoside(5′)oligophospho(5′)nucleosides (NpnN) by Sillero, María A. Günther, Socorro, Sónia, Baptista, María J., del Valle, Mercedes, de Diego, Anabel, Sillero, Antonio

“…The capacity of Escherichia coli poly(A) polymerase to adenylylate the 3′‐OH residue of a variety of nucleosides, nucleoside 5′‐phosphates and dinucleotides of…”
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Bisphosphonates activate the 5-fluorouracil/uracil phosphoribosyltransferase activity present in Saccharomyces cerevisiae cell extracts by Pérez-Zúñiga, Francisco J., Günther Sillero, María A., Sillero, Antonio

“…Most of the effects described for bisphosphonates (pC(R1)(R2)p) are related, directly or indirectly with a pyrophosphate moiety. Bisphosphonates are (i)…”
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Metabolic fate of AMP, IMP, GMP and XMP in the cytosol of rat brain: an experimental and theoretical analysis by Torrecilla, Amparo, Marques, Agostinho F. P., Buscalioni, Rafael D., Oliveira, Jorge M. A., Teixeira, Natercia A., Atencia, Eva Ana, Günther Sillero, María A., Sillero, Antonio

“…A systematic study of the metabolic fate of AMP, IMP, GMP and XMP (NMP) in the presence of cytosol from rat brain is here presented; the kinetics of both…”
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