Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet

This study aimed to investigate the effects of a high-fat and cholesterol diet (HFCD) on rats’ gastric mucosa. In the study, a total of 16 (40-day-old Sprague Dawley) male rats were used and randomly divided into two groups (each consisted of eight rats). The rats in control group had no implementat...

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Bibliographic Details
Published in:	Veterinary research forum Vol. 14; no. 6; pp. 309 - 315
Main Authors:	Gündoǧdu, Habibe, Sari, Ebru Karada&gcaron
Format:	Journal Article
Language:	English
Published:	Urmia Veterinary Research Forum 01-06-2023 Urmia University Press
Subjects:	Animals Antibodies Cholesterol High cholesterol diet High fat diet Investigations Nutrition research Original Software Somatostatin Stomach Ulcers United Kingdom > UK United States > US Germany
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Summary:	This study aimed to investigate the effects of a high-fat and cholesterol diet (HFCD) on rats’ gastric mucosa. In the study, a total of 16 (40-day-old Sprague Dawley) male rats were used and randomly divided into two groups (each consisted of eight rats). The rats in control group had no implementations other than normal feeding. For 10 weeks, rats in a high-fat with cholesterol diet group had daily energy amounts provided by pellet feed mixed with 65.00% butter and 2.00% cholesterol. Before beginning the study and at the end, rats live weight was recorded and their blood samples were taken for biochemical analyses. Hematoxylin and Eosin and Crossman’s triple staining techniques were used to investigate the general structure of gastric tissue. The rats fed with HFCD had statistically significant increases in live weight and total cholesterol values, and were identified to have gastric tissue degeneration. The rats’ gastric tissue in control group had more intense somatostatin (SST) immunoreactivity in parietal and chief cells than the HFCD group. It was determined that feeding with the HFCD has a negative effect on SST secretion in rats and hence, this may have important areas of use such as in gastric cancer treatment and preventing complications linked to gastric diseases.
ISSN:	2008-8140 2322-3618
DOI:	10.30466/vrf.2022.551598.3441