Unremitting cell proliferation in the secretory phase of eutopic endometriosis: involvement of pAkt and pGSK3β

Endometriosis is linked to altered cell proliferation and stem cell markers c-kit/stem cell factor (SCF) in ectopic endometrium. Our aim was to investigate whether c-kit/SCF also plays a role in eutopic endometrium. Eutopic endometrium obtained from 35 women with endometriosis and 25 fertile eumenor...

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Published in:	Reproductive sciences (Thousand Oaks, Calif.) Vol. 22; no. 4; p. 502
Main Authors:	Franco-Murillo, Yanira, Miranda-Rodríguez, José Antonio, Rendón-Huerta, Erika, Montaño, Luis F, Cornejo, Gerardo Velázquez, Gómez, Lucila Poblano, Valdez-Morales, Francisco Javier, Gonzalez-Sanchez, Ignacio, Cerbón, Marco
Format:	Journal Article
Language:	English
Published:	United States 01-04-2015
Subjects:	Adult Biopsy Case-Control Studies Cell Proliferation Endometriosis - enzymology Endometriosis - pathology Endometriosis - physiopathology Endometrium - enzymology Endometrium - pathology Endometrium - secretion Female Glycogen Synthase Kinase 3 - analysis Glycogen Synthase Kinase 3 beta Humans Immunohistochemistry Ki-67 Antigen - metabolism Phosphorylation Proto-Oncogene Proteins c-akt - analysis Proto-Oncogene Proteins c-kit - metabolism Signal Transduction Young Adult endometriosis Akt/GSK3β stem cell factor eutopic endometrium c-kit receptor
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Summary:	Endometriosis is linked to altered cell proliferation and stem cell markers c-kit/stem cell factor (SCF) in ectopic endometrium. Our aim was to investigate whether c-kit/SCF also plays a role in eutopic endometrium. Eutopic endometrium obtained from 35 women with endometriosis and 25 fertile eumenorrheic women was analyzed for in situ expression of SCF/c-kit, Ki67, RAC-alpha serine/threonine-protein kinase (Akt), phosphorylated RAC-alpha serine/threonin-protein kinase (pAkt), Glycogen synthase kinase 3 beta (GSK3β), and phosphorylated glycogen synthase kinase 3 beta (pGSK3β), throughout the menstrual cycle. Expression of Ki67 and SCF was higher in endometriosis than in control tissue (P < .05) and greater in secretory rather than proliferative (P < .01) endometrium in endometriosis. Expression of c-kit was also higher in endometriosis although similar in both phases. Expression of Akt and GSK3β was identical in all samples and cycle phases, whereas pAkt and pGSK3β, opposed to control tissue, remained overexpressed in the secretory phase in endometriosis. Unceasing cell proliferation in the secretory phase of eutopic endometriosis is linked to deregulation of c-kit/SCF-associated signaling pathways.
ISSN:	1933-7205
DOI:	10.1177/1933719114549843