Search Results - "Fyodor D Urnov"

Prime Time for Genome Editing? by Urnov, Fyodor D

“…Protein engineering has yielded a new, potent tool — in the form of a chimeric enzyme — for correcting genetic mutations. Proof of principle that this enzyme…”
Get full text

Generation of Isogenic Pluripotent Stem Cells Differing Exclusively at Two Early Onset Parkinson Point Mutations by Soldner, Frank, Laganière, Josée, Cheng, Albert W., Hockemeyer, Dirk, Gao, Qing, Alagappan, Raaji, Khurana, Vikram, Golbe, Lawrence I., Myers, Richard H., Lindquist, Susan, Zhang, Lei, Guschin, Dmitry, Fong, Lauren K., Vu, B. Joseph, Meng, Xiangdong, Urnov, Fyodor D., Rebar, Edward J., Gregory, Philip D., Zhang, H. Steve, Jaenisch, Rudolf

“…Patient-specific induced pluripotent stem cells (iPSCs) derived from somatic cells provide a unique tool for the study of human disease, as well as a promising…”
Get full text

Genome Editing of Isogenic Human Induced Pluripotent Stem Cells Recapitulates Long QT Phenotype for Drug Testing by Wang, Yongming, PhD, Liang, Ping, MD, PhD, Lan, Feng, PhD, Wu, Haodi, PhD, Lisowski, Leszek, PhD, Gu, Mingxia, MD, PhD, Hu, Shijun, PhD, Kay, Mark A., MD, PhD, Urnov, Fyodor D., PhD, Shinnawi, Rami, PhD, Gold, Joseph D., PhD, Gepstein, Lior, MD, PhD, Wu, Joseph C., MD, PhD

“…Abstract Background Human induced pluripotent stem cells (iPSCs) play an important role in disease modeling and drug testing. However, the current methods are…”
Get full text

Targeted Genome Editing Across Species Using ZFNs and TALENs by Wood, Andrew J., Lo, Te-Wen, Zeitler, Bryan, Pickle, Catherine S., Ralston, Edward J., Lee, Andrew H., Amora, Rainier, Miller, Jeffrey C., Leung, Elo, Meng, Xiangdong, Zhang, Lei, Rebar, Edward J., Gregory, Philip D., Urnov, Fyodor D., Meyer, Barbara J.

“…Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with…”
Get full text

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases by Amora, Ranier, Miller, Jeffrey C, Mitalipova, Maisam, Katibah, George E, Rebar, Edward J, Urnov, Fyodor D, Jaenisch, Rudolf, Gregory, Philip D, Boydston, Elizabeth A, Beard, Caroline, Hockemeyer, Dirk, Zeitler, Bryan, Meng, Xiangdong, Soldner, Frank, Gao, Qing, Zhang, Lei, DeKelver, Russell C

“…Hockemeyer et al . demonstrate targeted genetic modification of three genes in human embryonic stem cells and induced pluripotent stem cells using zinc-finger…”
Get full text

In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration by Cristea, Sandra, Freyvert, Yevgeniy, Santiago, Yolanda, Holmes, Michael C., Urnov, Fyodor D., Gregory, Philip D., Cost, Gregory J.

“…Targeted DNA integration is commonly used to eliminate position effects on transgene expression. Integration can be targeted to specific sites in the genome…”
Get full text

Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery by Genovese, Pietro, Galli, Cesare, Gregory, Philip D, Naldini, Luigi, Beausejour, Christian M, Colleoni, Silvia, Urnov, Fyodor D, Kim, Kenneth A, Ando, Dale, Lombardo, Angelo, Lee, Ya-Li, Holmes, Michael C

“…Achieving the full potential of zinc-finger nucleases (ZFNs) for genome engineering in human cells requires their efficient delivery to the relevant cell…”
Get full text

Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases by Geurts, Aron M, Cost, Gregory J, Freyvert, Yevgeniy, Zeitler, Bryan, Miller, Jeffrey C, Choi, Vivian M, Jenkins, Shirin S, Wood, Adam, Cui, Xiaoxia, Meng, Xiangdong, Vincent, Anna, Lam, Stephen, Michalkiewicz, Mieczyslaw, Schilling, Rebecca, Foeckler, Jamie, Kalloway, Shawn, Weiler, Hartmut, Ménoret, Séverine, Anegon, Ignacio, Davis, Gregory D, Zhang, Lei, Rebar, Edward J, Gregory, Philip D, Urnov, Fyodor D, Jacob, Howard J, Buelow, Roland

“…The toolbox of rat genetics currently lacks the ability to introduce site-directed, heritable mutations into the genome to create knockout animals. By using…”
Get full text

LRRK2 mutations cause mitochondrial DNA damage in iPSC-derived neural cells from Parkinson's disease patients: Reversal by gene correction by Sanders, Laurie H, Laganière, Josée, Cooper, Oliver, Mak, Sally K, Vu, B. Joseph, Huang, Y. Anne, Paschon, David E, Vangipuram, Malini, Sundararajan, Ramya, Urnov, Fyodor D, Langston, J. William, Gregory, Philip D, Zhang, H. Steve, Greenamyre, J. Timothy, Isacson, Ole, Schüle, Birgitt

“…Abstract Parkinson's disease associated mutations in leucine rich repeat kinase 2 ( LRRK2 ) impair mitochondrial function and increase the vulnerability of…”
Get full text

Genetic and molecular identification of three human TPP1 functions in telomerase action: recruitment, activation, and homeostasis set point regulation by Sexton, Alec N, Regalado, Samuel G, Lai, Christine S, Cost, Gregory J, O'Neil, Colleen M, Urnov, Fyodor D, Gregory, Philip D, Jaenisch, Rudolf, Collins, Kathleen, Hockemeyer, Dirk

“…Telomere length homeostasis is essential for the long-term survival of stem cells, and its set point determines the proliferative capacity of differentiated…”
Get full text

Precise genome modification in the crop species Zea mays using zinc-finger nucleases by Shukla, Vipula K, Doyon, Yannick, Miller, Jeffrey C, DeKelver, Russell C, Moehle, Erica A, Worden, Sarah E, Mitchell, Jon C, Arnold, Nicole L, Gopalan, Sunita, Meng, Xiangdong, Choi, Vivian M, Rock, Jeremy M, Wu, Ying-Ying, Katibah, George E, Zhifang, Gao

“…Agricultural biotechnology is limited by the inefficiencies of conventional random mutagenesis and transgenesis. Because targeted genome modification in plants…”
Get full text

Piloting an integrated SARS-CoV-2 testing and data system for outbreak containment among college students: A prospective cohort study by Packel, Laura, Reingold, Arthur, Hunter, Lauren, Facente, Shelley, Li, Yi, Harte, Anna, Nicolette, Guy, Urnov, Fyodor D, Lu, Michael, Petersen, Maya

“…Colleges and universities across the country are struggling to develop strategies for effective control of COVID-19 transmission as students return to campus…”
Get full text

Rapid and efficient clathrin-mediated endocytosis revealed in genome-edited mammalian cells by Drubin, David G, Lee, Andrew H, Cheng, Aaron T, Doyon, Yannick, Vo, Thuy D, Miller, Jeffrey C, Cherone, Jennifer M, Cheng, Jackie, Santiago, Yolanda, Urnov, Fyodor D, Paschon, David E, Doyon, Jeffrey B, Zhang, Lei, Rebar, Edward J, Gregory, Philip D, Zeitler, Bryan

“…Genome editing mediated by zinc finger nucleases can be used to generate fluorescently labelled proteins that are expressed at endogenous levels from their…”
Get full text

Site-specific genome editing in Plasmodium falciparum using engineered zinc-finger nucleases by Straimer, Judith, Lee, Marcus C S, Lee, Andrew H, Zeitler, Bryan, Williams, April E, Pearl, Jocelynn R, Zhang, Lei, Rebar, Edward J, Gregory, Philip D, Llinás, Manuel, Urnov, Fyodor D, Fidock, David A

“…This paper reports genetic manipulation of the malaria parasite Plasmodium falciparum with zinc-finger nucleases. It demonstrates gene disruption as well as…”
Get full text

Disruption of the BCL11A Erythroid Enhancer Reactivates Fetal Hemoglobin in Erythroid Cells of Patients with β-Thalassemia Major by Psatha, Nikoletta, Reik, Andreas, Phelps, Susan, Zhou, Yuanyue, Dalas, Demetri, Yannaki, Evangelia, Levasseur, Dana N., Urnov, Fyodor D., Holmes, Michael C., Papayannopoulou, Thalia

“…In the present report, we carried out clinical-scale editing in adult mobilized CD34+ hematopoietic stem and progenitor cells (HSPCs) using zinc-finger…”
Get full text

Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases by Santiago, Yolanda, Chan, Edmond, Liu, Pei-Qi, Orlando, Salvatore, Zhang, Lin, Urnov, Fyodor D, Holmes, Michael C, Guschin, Dmitry, Waite, Adam, Miller, Jeffrey C, Rebar, Edward J, Gregory, Philip D, Klug, Aaron, Collingwood, Trevor N

“…Gene knockout is the most powerful tool for determining gene function or permanently modifying the phenotypic characteristics of a cell. Existing methods for…”
Get full text

Long-Term Engraftment and Fetal Globin Induction upon BCL11A Gene Editing in Bone-Marrow-Derived CD34 + Hematopoietic Stem and Progenitor Cells by Chang, Kai-Hsin, Smith, Sarah E, Sullivan, Timothy, Chen, Kai, Zhou, Qianhe, West, Jason A, Liu, Mei, Liu, Yingchun, Vieira, Benjamin F, Sun, Chao, Hong, Vu P, Zhang, Mingxuan, Yang, Xiao, Reik, Andreas, Urnov, Fyodor D, Rebar, Edward J, Holmes, Michael C, Danos, Olivier, Jiang, Haiyan, Tan, Siyuan

“…To develop an effective and sustainable cell therapy for sickle cell disease (SCD), we investigated the feasibility of targeted disruption of the gene, either…”
Get full text

Robotic RNA extraction for SARS-CoV-2 surveillance using saliva samples by Hamilton, Jennifer R, Stahl, Elizabeth C, Tsuchida, Connor A, Lin-Shiao, Enrique, Tsui, C Kimberly, Pestal, Kathleen, Gildea, Holly K, Witkowsky, Lea B, Moehle, Erica A, McDevitt, Shana L, McElroy, Matthew, Keller, Amanda, Sylvain, Iman, Hirsh, Ariana, Ciling, Alison, Ehrenberg, Alexander J, Ringeisen, Bradley R, Huberty, Garth, Urnov, Fyodor D, Giannikopoulos, Petros, Doudna, Jennifer A

“…Saliva is an attractive specimen type for asymptomatic surveillance of COVID-19 in large populations due to its ease of collection and its demonstrated utility…”
Get full text

Improved specificity of TALE-based genome editing using an expanded RVD repertoire by Miller, Jeffrey C, Zhang, Lei, Xia, Danny F, Campo, John J, Ankoudinova, Irina V, Guschin, Dmitry Y, Babiarz, Joshua E, Meng, Xiangdong, Hinkley, Sarah J, Lam, Stephen C, Paschon, David E, Vincent, Anna I, Dulay, Gladys P, Barlow, Kyle A, Shivak, David A, Leung, Elo, Kim, Jinwon D, Amora, Rainier, Urnov, Fyodor D, Gregory, Philip D, Rebar, Edward J

“…This study extends the natural code by which transcription activation–like effector nucleases (TALENs) recognize DNA and uses the resulting expanded repertoire…”
Get full text

Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases by Moehle, E.A, Rock, J.M, Lee, Y.L, Jouvenot, Y, DeKelver, R.C, Gregory, P.D, Urnov, F.D, Holmes, M.C

“…Efficient incorporation of novel DNA sequences into a specific site in the genome of living human cells remains a challenge despite its potential utility to…”
Get full text