A Study of the Disruptive Effect of the Acetate Fraction of Punica granatum Extract on Cryptococcus Biofilms
Cryptococcosis, caused by yeasts of the genus , is an infectious disease with a worldwide distribution. and are the species that commonly cause this disease in humans; however, infections caused by , especially in immunocompromised patients, are increasingly being reported. Owing to the increase in...
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| Published in: | Frontiers in microbiology Vol. 11; p. 568258 |
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| Main Authors: | , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
Frontiers Media S.A
18-01-2021
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Cryptococcosis, caused by yeasts of the genus
, is an infectious disease with a worldwide distribution.
and
are the species that commonly cause this disease in humans; however, infections caused by
, especially in immunocompromised patients, are increasingly being reported. Owing to the increase in the resistance of fungi to antifungals, and a lack of treatment options, it is important to seek new therapeutic alternatives such as natural products. Among these are plant species such as
, which is used in folk medicine to treat various diseases. This study aimed to evaluate the activity of the acetate fraction of
leaf extract against environmental and clinical isolates of
. Three environmental isolates of
, PMN, PMA, and PJL II, isolated from soils of different municipalities in the state of Maranhão, a clinical isolate,
, from a patient with neurocryptococcosis, and a standard strain of
(ATCC 32068) were used. The minimum and fractional inhibitory concentrations (MIC and FIC, respectively) and time-kill curve of the extract and fluconazole were determined to assess the susceptibility profile of the fungal isolates. Larvae of
were infected with
strains, and the effects of acetate fraction of
extract and fluconazole on the survival and fungal burden were determined. The extract activity was tested against pre-formed biofilms. The acetate fraction of
extract showed promising antifungal activity against all the species of
evaluated in this study, with an MIC value lower than that of fluconazole. The indices obtained in the FIC test indicated that the antimicrobial effect of the combination of the extract and antifungal was indifferent for 80% of the isolates. The
acetate fraction reduced the pre-formed biofilm of some isolates, showing better activity than fluconazole, which is consistent with results from fluorescence microscopy. This is the first study on the use of
and its ability to inhibit
biofilms; therefore, further studies and tests are needed to investigate the components and mechanism of action of
against cryptococcosis agents. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors have contributed equally to this work Edited by: Hemda Garelick, Middlesex University, United Kingdom Reviewed by: Ashutosh Singh, University of Lucknow, India; Janaina De Cássia Orlandi Sardi, University of Campinas, Brazil This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology |
| ISSN: | 1664-302X 1664-302X |
| DOI: | 10.3389/fmicb.2020.568258 |