Search Results - "Fulghum, John R."

In Vitro Studies of Cross-resistance Mutations against Two Hepatitis C Virus Serine Protease Inhibitors, VX-950 and BILN 2061 by Lin, Chao, Gates, Cynthia A., Rao, B. Govinda, Brennan, Debra L., Fulghum, John R., Luong, Yu-Ping, Frantz, J. Daniel, Lin, Kai, Ma, Sue, Wei, Yun-Yi, Perni, Robert B., Kwong, Ann D.

“…VX-950 is a potent, small molecule, peptidomimetic inhibitor of the hepatitis C virus (HCV) NS3·4A serine protease and has recently been shown to possess…”
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Structure of Mitogen-activated Protein Kinase-activated Protein (MAPKAP) Kinase 2 Suggests a Bifunctional Switch That Couples Kinase Activation with Nuclear Export by Meng, Wuyi, Swenson, Lora L., Fitzgibbon, Matthew J., Hayakawa, Koto, ter Haar, Ernst, Behrens, Anne E., Fulghum, John R., Lippke, Judith A.

“…MAPK-activated protein kinase 2 (MAPKAPK2), one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory…”
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In vitro resistance studies of hepatitis C virus serine protease inhibitors, VX-950 and BILN 2061: structural analysis indicates different resistance mechanisms by Lin, Chao, Lin, Kai, Luong, Yu-Ping, Rao, B Govinda, Wei, Yun-Yi, Brennan, Debra L, Fulghum, John R, Hsiao, Hsun-Mei, Ma, Sue, Maxwell, John P, Cottrell, Kevin M, Perni, Robert B, Gates, Cynthia A, Kwong, Ann D

“…We have used a structure-based drug design approach to identify small molecule inhibitors of the hepatitis C virus (HCV) NS3.4A protease as potential…”
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Nonstructural protein 5A (NS5A) and human replication protein A increase the processivity of hepatitis C virus NS5B polymerase activity in vitro by Mani, Nagraj, Yuzhakov, Alexander, Yuzhakov, Olga, Coll, Joyce T, Black, Jim, Saxena, Kumkum, Fulghum, John R, Lippke, Judith A, Rao, B Govinda, Rijnbrand, Rene, Kwong, Ann D

“…The precise role(s) and topological organization of different factors in the hepatitis C virus (HCV) RNA replication complex are not well understood. In order…”
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Screening factors effecting a response in soluble protein expression: Formalized approach using design of experiments by Swalley, Susanne E., Fulghum, John R., Chambers, Stephen P.

“…We have integrated high-throughput expression and purification with quantitative protein analysis to identify factors influencing protein production…”
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High-throughput screening for soluble recombinant expressed kinases in Escherichia coli and insect cells by Chambers, Stephen P, Austen, Douglas A, Fulghum, John R, Kim, Walter M

“…We have constructed a dual expression vector for the production of recombinant proteins in both Escherichia coli and insect cells. In this vector, the…”
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Inhibition of IMPDH by Mycophenolic Acid:  Dissection of Forward and Reverse Pathways Using Capillary Electrophoresis by Fleming, Mark A, Chambers, Stephen P, Connelly, Patrick R, Nimmesgern, Elmar, Fox, Ted, Bruzzese, Frank J, Hoe, Stephen T, Fulghum, John R, Livingston, David J, Stuver, Cameron M, Sintchak, Michael D, Wilson, Keith P, Thomson, John A

“…The objective of this work was to contribute to the understanding of mechanisms for IMPDH inhibition. We over-expressed hamster type II IMPDH in Escherichia…”
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E. coli and insect cell expression, automated purification and quantitative analysis by Chambers, Stephen P, Fulghum, John R, Austen, Douglas A, Lu, Fan, Swalley, Susanne E

“…The production of recombinant proteins usually involves the exploration of a wide variety of expression and purification methodologies in the pursuit of a…”
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Biochemical Analysis of the Modular Enzyme Inosine 5′-Monophosphate Dehydrogenase by Nimmesgern, Elmar, Black, James, Futer, Olga, Fulghum, John R., Chambers, Stephen P., Brummel, Christopher L., Raybuck, Scott A., Sintchak, Michael D.

“…Two prominent domains have been identified in the X-ray crystal structure of inosine-5′-monophosphate dehydrogenase (IMPDH), a core domain consisting of an α/β…”
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In Vitro Resistance Studies of Hepatitis C Virus Serine Protease Inhibitors, VX-950 and BILN 2061 by Lin, Chao, Lin, Kai, Luong, Yu-Ping, Rao, B. Govinda, Wei, Yun-Yi, Brennan, Debra L., Fulghum, John R., Hsiao, Hsun-Mei, Ma, Sue, Maxwell, John P., Cottrell, Kevin M., Perni, Robert B., Gates, Cynthia A., Kwong, Ann D.

“…We have used a structure-based drug design approach to identify small molecule inhibitors of the hepatitis C virus (HCV) NS3·4A protease as potential…”
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Nitrogen-15 NMR relaxation studies of the FK506 binding protein: Backbone dynamics of the uncomplexed receptor by Cheng, Jya Wei, Lepre, Christopher A, Chambers, Stephen P, Fulghum, John R, Thomson, John A, Moore, Jonathan M

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Purification and characterization of the NS3 serine protease domain of hepatitis C virus expressed in Saccharomyces cerevisiae by Markland, W, Petrillo, RA, Fitzgibbon, M, Fox, T, McCarrick, R, McQuaid, T, Fulghum, JR, Chen, W, Fleming, MA, Thomson, JA, Chambers, SP

“…W Markland, RA Petrillo, M Fitzgibbon, T Fox, R McCarrick, T McQuaid, JR Fulghum, W Chen, MA Fleming, JA Thomson and SP Chambers Vertex Pharmaceuticals Inc.,…”
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Expression and Purification of Human Interleukin-1β Converting Enzyme fromTrichoplusia niInsect Cells Using a Baculovirus Expression System by Chen, Wenyong, Raybuck, Scott A., Fulghum, John R., Petrillo, Richard A., Margolin, Nara, Chambers, Stephen P.

“…Employing a baculovirus expression system, human interleukin-1β converting enzyme (ICE) has been expressed inTrichoplusia niHigh-Five insect cells and…”
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