Search Results - "Fukusho, Akio"

Biological properties of bovine viral diarrhea virus quasispecies detected in the RK13 cell line by Muhsen, Mahmod, Aoki, Hiroshi, Ikeda, Hidetoshi, Fukusho, Akio

“…The rabbit kidney cell line RK13 has been reported to be contaminated with noncytopathogenic (ncp) bovine viral diarrhea virus (BVDV). Persistent infection was…”
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Field Distribution of END Phenomenon-Negative Bovine Viral Diarrhea Virus by NISHINE, Kaoru, AOKI, Hiroshi, SAKODA, Yoshihiro, FUKUSHO, Akio

“…Field isolates of BVDV which do not show the exaltation of Newcastle disease virus (END) phenomenon (END–) are rarely reported. In this study, 45 BVDV field…”
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Competitive virus assay method for titration of noncytopathogenic bovine viral diarrhea viruses (END+ and END− viruses) by Muhsen, Mahmod, Ohi, Kota, Aoki, Hiroshi, Ikeda, Hidetoshi, Fukusho, Akio

“…► We developed a new assay method for non-cytopathogenic bovine viral diarrhea virus. ► It was named as competitive virus assay method (CVA method). ► It is…”
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Methods to select suitable fetal bovine serum for use in quality control assays for the detection of adventitious viruses from biological products by Kozasa, Takashi, Aoki, Hiroshi, Nakajima, Nao, Fukusho, Akio, Ishimaru, Masatoshi, Nakamura, Shigeyuki

“…Production of biological products, especially vaccines, usually requires materials derived from animals, and there are always risks that animal pathogens…”
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Classical swine fever: the global situation by Edwards, Steven, Fukusho, Akio, Lefèvre, Pierre-Charles, Lipowski, Andrzej, Pejsak, Zygmunt, Roehe, Paulo, Westergaard, Jørgen

“…A historical and current perspective is given of classical swine fever and its impact on pig production in different regions of the world. Data were obtained…”
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Development and evaluation of indirect enzyme-linked immunosorbent assay for a screening test to detect antibodies against classical swine fever virus by Sakoda, Yoshihiro, Wakamoto, Hiroaki, Tamura, Tehpin, Nomura, Takushi, Naito, Michiko, Aoki, Hiroshi, Morita, Hiroshi, Kida, Hiroshi, Fukusho, Akio

“…An indirect enzyme-linked immunosorbent assay (ELISA) was developed for a screening test to detect antibodies against classical swine fever virus (CSFV). Viral…”
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Genetic heterogeneity of porcine and ruminant pestiviruses mainly isolated in Japan by Sakoda, Yoshihiro, Ozawa, Shin-ichi, Damrongwatanapokin, Sudarat, Sato, Mitsuo, Ishikawa, Kiyoyasu, Fukusho, Akio

“…The genetic variability of porcine and ruminant pestiviruses was studied by comparative nucleotide sequence analysis of 73 isolates (42 porcine and 31…”
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Establishment and characterization of a porcine kidney cell line, FS-L3, which forms unique multicellular domes in serum-free culture by Sakoda, Y, Fukusho, A

“…A stable porcine kidney epithelial cell line, FS-L3, was established and maintained in Eagle's minimum essential medium containing 0.295% tryptose phosphate…”
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Porcine circovirus type 2 induces a strong cytopathic effect in the serum-free culture cell line CPK-NS by Hosono, Shuhei, Shiokawa, Mai, Kobayashi, Tsubasa, Fukusho, Akio, Aoki, Hiroshi

“…•CPK-NS cells passaged after inoculation with porcine circovirus type 2 (PCV2) showed cell detachment depending on the virus concentration.•The cell detachment…”
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Induction of antibodies in mice by a recombinant baculovirus expressing pseudorabies virus glycoprotein B in mammalian cells by Aoki, Hiroshi, Sakoda, Yoshihiro, Jukuroki, Keiko, Takada, Ayato, Kida, Hiroshi, Fukusho, Akio

“…The glycoprotein gB of pseudorabies virus (PrV) was expressed in various mammalian cells by a recombinant baculovirus carrying the PrV gB gene under the…”
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Cytopathogenicity of Classical Swine Fever Viruses that do not Show the Exaltation of Newcastle Disease Virus is Associated with Accumulation of NS3 in Serum-Free Cultured Cell Lines by AOKI, Hiroshi, SAKODA, Yoshihiro, NAKAMURA, Shigeyuki, SUZUKI, Shoko, FUKUSHO, Akio

“…Pestiviruses can be distinguished as two biotypes, cytopathogenic (cp) and noncytopathogenic (noncp), by the morphological changes that they induce during…”
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END-phenomenon negative bovine viral diarrhea virus that induces the host's innate immune response supports propagation of BVDVs with different immunological properties by Shiokawa, Mai, Omatsu, Tsutomu, Katayama, Yukie, Nishine, Kaoru, Fujimoto, Yuri, Uchiyama, Shiori, Kameyama, Ken-ichiro, Nagai, Makoto, Mizutani, Tetsuya, Sakoda, Yoshihiro, Fukusho, Akio, Aoki, Hiroshi

“…Our previous study reported that persistently infected (PI) cattle of bovine viral diarrhea virus (BVDV) have co-infected with BVDV/END− and /END+ that promote…”
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Establishment of a serum-free culture cell line, CPK-NS, which is useful for assays of classical swine fever virus by Sakoda, Yoshihiro, Hikawa, Mifuyu, Tamura, Tehpin, Fukusho, Akio

“…A stable porcine kidney cell line, CPK-NS, was established and maintained in serum-free culture. A cytopathic effect (CPE) was observed clearly in CPK-NS cells…”
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Characterization of classical swine fever virus associated with defective interfering particles containing a cytopathogenic subgenomic RNA isolated from wild boar by Aoki, H. (Japan. Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Tokyo. National Veterinary Assay Lab.), Ishikawa, K, Sakoda, Y, Sekiguchi, H, Kodama, M, Suzuki, S, Fukusho, A

“…Classical swine fever virus (CSFV) strain WB82, isolated from a wild boar in 1982, induced a distinct cytopathic effect (CPE) in primary swine testicle cell…”
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A new assay for classical swine fever virus based on cytopathogenicity in porcine kidney cell line FS-L3 by Sakoda, Yoshihiro, Yamaguchi, Osamu, Fukusho, Akio

“…A new assay termed the dome disappearance method for classical swine fever virus (CSFV) using FS-L3 cells with serum-free culture medium was developed. The…”
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Variation from cytopathogenic biotype to non-cytopathogenic biotype is correlated with the deletion of cellular sequence from bovine viral diarrhea viruses by Nakamura, S. (Japan. Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Tokyo. National Veterinary Assay Lab.), Sakamoto, K, Sakoda, Y, Shimazaki, T, Inoue, Y, Ogawa, N, Fukusho, A

“…Non-cytopathogenic (NCP) viruses of bovine viral diarrhea (BVD) virus were detected at a low ratio by the reverse plaque formation method from virus samples…”
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African Horsesickness Virus Infection in Adult Mice by SAKAMOTO, Kenichi, KAWASHIMA, Kenji, NARITA, Minoru, KAMADA, Masanobu, FUKUSHO, Akio

“…Adult BALB/c mice were inoculated intranasally with African horsesickness virus serotype 4 (AHSV-4), the attenuated-vaccine strain. The 4-week-old mice died…”
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Development and evaluation of indirect enzyme-linked immunosorbent assay for a screening test to detect antibodies against classical swine fever virus by Yoshihiro Sakoda, Hiroaki Wakamoto, Tehpin Tamura, Takushi Nomura, Michiko Naito, Hiroshi Aoki, Hiroshi Morita, Hiroshi Kida, Akio Fukusho

“…[Abstract] An indirect enzyme-linked immunosorbent assay (ELISA) was developed for a screening test to detect antibodies against classical swine fever virus…”
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牛ウイルス性下痢ウイルス感染牛精巣培養細胞におけるオルビウイルスの増殖増強作用 by 中村, 成幸, 嶋崎, 智章, 坂本, 研一, 福所, 秋雄, 井上, 剛光, 小川, 信雄

“…非細胞病原性(NCP)牛ウイルス性下痢ウイルス(BVDV)感染牛精巣培養(BT)細胞に7種類のオルビウイルスを重感染させたところ, 全てのウイルスがCPEを発現するとともにBVDV非感染BT細胞に比べウイルスの増殖が100倍以上増強された. NCP ・BVDV…”
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NSI遺伝子を標的とするRT-PCR法によるアフリカ馬疫ウイルスの検出 by 水越, 紀子, 坂本, 研一, 岩田, 晃, 上田, 進, 鎌田, 正信, 福所, 秋雄

“…アフリカ馬疫ウイルス(AHSV)血清型4(ワクチン株)の第5分節RNA(NSI遺伝子)に対するプライマーを用い, グアニジンチオシアネート・フェノール・クロロホルム法によって感染細胞から抽出したウイルスRNA(mRNA・dsRNA)を鋳型にRT-PCRを実施し, AHSVの効率的な検出条件について検討した…”
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