Adhesion of germlings of Botrytis cinerea

Adhesion of conidia and germlings of the facultative plant parasite Botrytis cinerea occurs in two distinct stages. The first stage, which occurs immediately upon hydration of conidia and is characterized by relatively weak adhesive forces, appears to involve hydrophobic interactions (R. P. Doss, S....

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Bibliographic Details
Published in:	Applied and Environmental Microbiology Vol. 61; no. 1; pp. 260 - 265
Main Authors:	Doss, R.P. (Horticulture Crops Research Unit, USDA, ARS, Corvallis, OR.), Potter, S.W, Soeldner, A.H, Christian, J.K, Fukunaga, L.E
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-01-1995
Subjects:	Bacteriology Biological and medical sciences Botany BOTRYTIS CINEREA Cell Adhesion - drug effects Cell Adhesion - physiology Enzymes - pharmacology Epoxy Compounds - pharmacology ESPORAS Flowers & plants Fundamental and applied biological sciences. Psychology Fungi LYCOPERSICON ESCULENTUM MICELIO Microbiology Microscopy, Electron, Scanning Mitosporic Fungi - physiology MYCELIUM Parasites Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Plants - microbiology POLIMEROS POLYMERE PROPIEDADES MECANICAS PROPRIETE MECANIQUE SECRECION SECRETION SPORE VERRE VIDRIO Fungi Substrate Botrytis cinerea Plant pathogen Infectivity Germination Fungi Imperfecti Adhesion Thallophyta Conidia
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Summary:	Adhesion of conidia and germlings of the facultative plant parasite Botrytis cinerea occurs in two distinct stages. The first stage, which occurs immediately upon hydration of conidia and is characterized by relatively weak adhesive forces, appears to involve hydrophobic interactions (R. P. Doss, S. W. Potter, G. A. Chastagner, and J. K. Christian, Appl. Environ. Microbiol. 59:1786-1791, 1993). The second stage of adhesion, delayed adhesion, occurs after viable conidia have been incubated for several hours under conditions that promote germination. At this time, the germlings attach strongly to either hydrophobic or hydrophilic substrata. Delayed adhesion involves secretion of an ensheathing film that remains attached to the substratum upon physical removal of the germlings. This fungal sheath, which can be visualized by using interference-contrast light microscopy, scanning electron microscopy, or atomic force microscopy, is 25 to 60 nm thick in the region immediately adjacent to the germ tubes. Germlings are resistant to removal by boiling or by treatment with a number of hydrolytic enzymes, 2.0 M periodic acid, or 1.0 M sulfuric acid. They are readily removed by brief exposure to 1.25 N NaOH. A base-soluble material that adheres to culture flask walls in short-term liquid cultures of B. cinerea is composed of glucose (about 30%), galactosamine (about 3%), and protein (30 to 44%)
Bibliography:	9534170 H20 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.61.1.260-265.1995