Search Results - "Fujioka, Motoji"

Catalytic Mechanism of Glycine N-Methyltransferase by Takata, Yoshimi, Huang, Yafei, Komoto, Junichi, Yamada, Taro, Konishi, Kiyoshi, Ogawa, Hirofumi, Gomi, Tomoharu, Fujioka, Motoji, Takusagawa, Fusao

“…Methyltransfer reactions are some of the most important reactions in biological systems. Glycine N-methyltransferase (GNMT) catalyzes the…”
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Crystal Structure of Guanidinoacetate Methyltransferase from Rat Liver: A Model Structure of Protein Arginine Methyltransferase by Komoto, Junichi, Huang, Yafei, Takata, Yoshimi, Yamada, Taro, Konishi, Kiyoshi, Ogawa, Hirofumi, Gomi, Tomoharu, Fujioka, Motoji, Takusagawa, Fusao

“…Guanidinoacetate methyltransferase (GAMT) is the enzyme that catalyzes the last step of creatine biosynthesis. The enzyme is found in abundance in the livers…”
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Functional Expression of Gastric H+,K+-ATPase and Site-directed Mutagenesis of the Putative Cation Binding Site and the Catalytic Center () by Asano, Shinji, Tega, Yasuhiro, Konishi, Kiyoshi, Fujioka, Motoji, Takeguchi, Noriaki

“…Gastric H+,K+-ATPase was functionally expressed in the human kidney HEK293 cell line. The expressed enzyme catalyzed ouabain-resistant K+-dependent ATP…”
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Catalytic Mechanism ofS-Adenosylhomocysteine Hydrolase by Takata, Yoshimi, Yamada, Taro, Huang, Yafei, Komoto, Junichi, Gomi, Tomoharu, Ogawa, Hirofumi, Fujioka, Motoji, Takusagawa, Fusao

“…S-Adenosylhomocysteine hydrolase (AdoHcyase) catalyzes the hydrolysis ofS-adenosylhomocysteine to form adenosine and homocysteine. On the bases of crystal…”
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Inhibition of S-Adenosylhomocysteine Hydrolase by Acyclic Sugar Adenosine Analogue d-Eritadenine by Huang, Yafei, Komoto, Junichi, Takata, Yoshimi, Powell, Douglas R., Gomi, Tomoharu, Ogawa, Hirofumi, Fujioka, Motoji, Takusagawa, Fusao

“…d-Eritadenine (DEA) is a potent inhibitor (IC50 = 7 nm) ofS-adenosyl-l-homocysteine hydrolase (AdoHcyase). Unlike cyclic sugar Ado analogue inhibitors,…”
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Effects of Site-directed Mutagenesis on Structure and Function of Recombinant Rat Liver S-Adenosylhomocysteine Hydrolase by Komoto, Junichi, Huang, Yafei, Gomi, Tomoharu, Ogawa, Hirofumi, Takata, Yoshimi, Fujioka, Motoji, Takusagawa, Fusao

“…A site-directed mutagenesis, D244E, ofS-adenosylhomocysteine hydrolase (AdoHcyase) changes drastically the nature of the protein, especially the NAD+binding…”
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Structure of the gene encoding rat neutrophil chemo-attractant Gro by Konishi, K, Takata, Y, Yamamoto, M, Yomogida, K, Watanabe, K, Tsurufuji, S, Fujioka, M

“…A cloned rat gro gene encoding the neutrophil chemo-attractant Gro was isolated from a lambda Charon4A rat genomic library, and the nucleotide (nt) sequence of…”
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Guanidinoacetate Methyltransferase in the Mouse: Extensive Expression in Sertoli Cells of Testis and in Microvilli of Caput Epididymis1 by Lee, Hang, Ogawa, Hirofumi, Fujioka, Motoji, Gerton, George L.

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Catalytic mechanism of S-adenosylhomocysteine hydrolase: Roles of His 54, Asp130, Glu155, Lys185, and Aspl89 by Yamada, Taro, Takata, Yoshimi, Komoto, Junichi, Gomi, Tomoharu, Ogawa, Hirofumi, Fujioka, Motoji, Takusagawa, Fusao

“…S-Adenosylhomocysteine hydrolase (AdoHcyase) catalyzes the hydrolysis of S-adenosylhomocysteine (AdoHcy) to form adenosine and homocysteine. The crystal…”
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Recombinant expression of rat and human Gro proteins in Escherichia coli by Konishi, K, Takata, Y, Watanabe, K, Date, T, Yamamoto, M, Murase, A, Yoshida, H, Suzuki, T, Tsurufuji, S, Fujioka, M

“…A full-length rat gro cDNA containing the signal sequence was inserted to a plasmid/phage vector pTD-lacs which had the Escherichia coli alkaline phosphatase…”
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Purification and characterization of cytosolic and mitochondrial serine hydroxymethyltransferases from rat liver by Ogawa, H, Fujioka, M

“…The cytosolic and mitochondrial forms of serine hydroxymethyltransferase [EC 2.1.2.1] were purified to homogeneity from a whole homogenate of rat liver without…”
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Serine hydroxymethyltransferase and threonine aldolase: are they identical? by Ogawa, Hirofumi, Gomi, Tomoharu, Fujioka, Motoji

“…Serine hydroxymethyltransferase, a pyridoxal phosphate-dependent enzyme, catalyses the interconversion of serine and glycine, both of which are major sources…”
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Crystal Structure of S-Adenosylhomocysteine Hydrolase from Rat Liver by Hu, Yongbo, Komoto, Junichi, Huang, Yafei, Gomi, Tomoharu, Ogawa, Hirofumi, Takata, Yoshimi, Fujioka, Motoji, Takusagawa, Fusao

“…The crystal structure of rat liver S-adenosyl-l-homocysteine hydrolase (AdoHcyase, EC 3.3.1.1) which catalyzes the reversible hydrolysis of…”
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The peptide sequences near the bound pyridoxal phosphate are conserved in serine dehydratase from rat liver, and threonine dehydratases from yeast and Escherichia coli by Ogawa, H, Konishi, K, Fujioka, M

“…The blocked amino-terminal residue of rat liver serine dehydratase was shown to be acetylalanine by analysis of an isolated amino-terminal peptide after…”
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Catalytic Mechanism of Guanidinoacetate Methyltransferase:  Crystal Structures of Guanidinoacetate Methyltransferase Ternary Complexes by Komoto, Junichi, Yamada, Taro, Takata, Yoshimi, Konishi, Kiyoshi, Ogawa, Hirofumi, Gomi, Tomoharu, Fujioka, Motoji, Takusagawa, Fusao

“…Guanidinoacetate methyltransferase (GAMT) is the enzyme that catalyzes the last step of creatine biosynthesis. The enzyme is found in abundance in the livers…”
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Recombinant Production and Biological Properties of Rat Cytokine-Induced Neutrophil Chemoattractants, GRO/CINC-2alpha, CINC-2beta and CINC-3 by Shibata, Futoshi, Konishi, Kiyoshi, Kato, Hideko, Komorita, Naruyasu, Al-Mokdad, Maher, Fujioka, Motoji, Nakagawa, Hideo

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Purification and properties of serine hydroxymethylase from soluble and mitochondrial fractions of rabbit liver by Fujioka, M

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Catalytic mechanism of S-adenosylhomocysteine hydrolase. Site-directed mutagenesis of Asp-130, Lys-185, Asp-189, and Asn-190 by Takata, Yoshimi, Yamada, Taro, Huang, Yafei, Komoto, Junichi, Gomi, Tomoharu, Ogawa, Hirofumi, Fujioka, Motoji, Takusagawa, Fusao

“…S-Adenosylhomocysteine hydrolase (AdoHcyase) catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine. On the bases of crystal…”
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Saccharopine Dehydrogenase by Fujioka, Motoji, Nakatani, Yoichi

“…In addition to a high degree of specificity toward its amino acid and keto acid substrates, saccharopine dehydrogenase (ε- N -( l -glutaryl-2)- l -lysine: NAD…”
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Inhibition of S-adenosylhomocysteine hydrolase by acyclic sugar adenosine analogue D-eritadenine. Crystal structure of S-adenosylhomocysteine hydrolase complexed with D-eritadenine by Huang, Yafei, Komoto, Junichi, Takata, Yoshimi, Powell, Douglas R, Gomi, Tomoharu, Ogawa, Hirofumi, Fujioka, Motoji, Takusagawa, Fusao

“…D-eritadenine (DEA) is a potent inhibitor (IC(50) = 7 nm) of S-adenosyl-l-homocysteine hydrolase (AdoHcyase). Unlike cyclic sugar Ado analogue inhibitors,…”
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