Chronic encephalomyelitis virus exhibits cellular tropism and evades pDCs by binding to sialylated integrins as the cell surface receptors

Chronic encephalomyelitis virus exhibits cellular tropism and evades pDCs by binding to sialylated integrins as the cell surface receptors

Theiler's murine encephalomyelitis virus (TMEV) causes a chronic demyelinating disease similar to multiple sclerosis in mice. Although sialic acids have been shown to be essential for TMEV attachment to the host, the surface receptor has not been identified. While type I interferons play a pivo...

Full description

Saved in:
Bibliographic Details
Published in:European journal of immunology Vol. 53; no. 10; pp. e2350452 - n/a
Main Authors: Takeda, Kazuya, Kaifu, Tomonori, Michihata, Ryunosuke, Kinugawa, Naotaka, Fujioka, Atushi, Tateno, Ayaka, Toshima, Kaoru, Kanoh, Hirotaka, Inamori, Kei‐Ichiro, Kamijo, Keiju, Himeda, Toshiki, Ohara, Yoshiro, Inokuchi, Jin‐Ichi, Nakamura, Akira
Format: Journal Article
Language:English
Published: Germany Wiley Subscription Services, Inc 01-10-2023
Subjects:
Cell membranes
Cell surface
Cell surface receptors
Cytoplasm
Demyelinating diseases
Demyelination
Dendritic cells
Encephalomyelitis
Integrin
Integrins
Multiple sclerosis
pDC
Sialic acid
Sialic acids
TMEV
Tropism
Virus attachment
Viruses
Sialic acid
Integrin
TMEV
pDC
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Theiler's murine encephalomyelitis virus (TMEV) causes a chronic demyelinating disease similar to multiple sclerosis in mice. Although sialic acids have been shown to be essential for TMEV attachment to the host, the surface receptor has not been identified. While type I interferons play a pivotal role in the elimination of the chronic infectious Daniel (DA) strain, the role of plasmacytoid dendritic cells (pDCs) is controversial. We herein found that TMEV binds to conventional DCs but not to pDCs. A glycomics analysis showed that the sialylated N‐glycan fractions were lower in pDCs than in conventional DCs, indicating that pDCs are not susceptible to TMEV infection due to the low levels of sialic acid. TMEV capsid proteins contain an integrin recognition motif, and dot blot assays showed that the integrin proteins bind to TMEV and that the viral binding was reduced in the desialylated αXβ2. αXβ2 protein suppressed TMEV replication in vivo, and TMEV co‐localized with integrin αM at the cell membrane and TLR 3 in the cytoplasm, suggesting that αM serves as the viral attachment and entry. These results show that the chronic encephalomyelitis virus utilizes sialylated integrins as cell surface receptors, leading to cellular tropism to evade pDC activation. TMEV binds to the open form αI‐domain containing integrins that undergo sialic acid (NeuAc) modifications on DCs. In contrast, because pDCs have reduced sialic acid modifications, integrins are not sialylated and TMEV evades recognition by pDCs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.202350452