Post-translational suppression of the high affinity IgE receptor expression on mast cells by an intestinal bacterium

Post-translational suppression of the high affinity IgE receptor expression on mast cells by an intestinal bacterium

Mast cells, which express the high-affinity IgE receptor (FcεRI) on their surface, play a crucial role in inducing allergic inflammation. Since mast cells are activated by crosslinking of FcεRI with IgE and allergens, the cell surface expression level of FcεRI is an important factor in determining t...

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Published in:Immunobiology (1979) Vol. 226; no. 2; p. 152056
Main Authors: Fukatsu, Sakino, Horinouchi, Hikari, Nagata, Shiho, Kamei, Risa, Tanaka, Daichi, Hong, Wonki, Kazami, Yui, Fujimori, Minami, Itoh, Kikuji, Momose, Yoshika, Kasakura, Kazumi, Hosono, Akira, Kaminogawa, Shuichi, Hanazawa, Shigemasa, Nakanishi, Yusuke, Takahashi, Kyoko
Format: Journal Article
Language:English
Published: Netherlands Elsevier GmbH 01-03-2021
Subjects:
Animals
Bacteroides
Bacteroides acidifaciens
Cells, Cultured
FcεRI
Female
Gastrointestinal Microbiome
Intestines - microbiology
Mast cell
Mast Cells - immunology
Mice
Mice, Inbred C57BL
Protein Processing, Post-Translational
Receptors, IgE - genetics
Receptors, IgE - immunology
PBS
Ab
BSA
SDS-PAGE
HRP
FcεRI
Mast cell
TLR
Bacteroides acidifaciens
SCFA
BMMC
BA
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Summary:Mast cells, which express the high-affinity IgE receptor (FcεRI) on their surface, play a crucial role in inducing allergic inflammation. Since mast cells are activated by crosslinking of FcεRI with IgE and allergens, the cell surface expression level of FcεRI is an important factor in determining the sensitivity to allergens. Recently, the involvement of gut microbiota in the prevalence and regulation of allergy has attracted attention but the precise underlying mechanisms are not fully understood. In this study, the effect of intestinal bacteria on cell surface expression of FcεRI was examined. Bacteroides acidifaciens type A 43 specifically suppressed cell surface expression of FcεRI on mouse bone marrow-derived mast cells (BMMCs) without reduction in FcεRI α and β-chain mRNA and total protein expression. The suppressive effect required sustained exposure to this bacterium, with a corresponding reduction in Erk activation. Inhibition of Erk decreased cell surface distribution of FcεRI in BMMCs, at least in part, through facilitated endocytosis of FcεRI. These results indicate that B. acidifaciens type A 43 suppresses cell surface expression of FcεRI on mast cells in a post-translational manner via inhibition of Erk. The suppression of FcεRI expression on mast cells by specific bacteria might be the underlying mechanism involved in the regulation of allergy by gut microbiota.
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content type line 23
ISSN:0171-2985
1878-3279
DOI:10.1016/j.imbio.2021.152056