Search Results - "Fu, Jinheng"
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Substrate sustained release-based high efficacy biosynthesis of GABA by Lactobacillus brevis NCL912
Published in Microbial cell factories (19-05-2018)“…Gamma-aminobutyric acid (GABA) plays a significant role in the food and drug industries. Our previous study established an efficient fed-batch fermentation…”
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Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA
Published in Metabolites (16-02-2023)“…Gamma-aminobutyric acid (GABA) is a functional metabolite in various organisms. Herein, a sensitivity intensified ninhydrin-based chromogenic system (SINICS),…”
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Engineering a recombination neutral protease I from Aspergillus oryzae to improve enzyme activity at acidic pH
Published in RSC advances (19-08-2020)“…Extracellular neutral proteases (NPs) in ( ) play a role in hydrolyzing soybean proteins into smaller peptides at pH about 7.5. The optimum pH of moromi…”
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Research on the Mechanism of Action of a Citrinin and Anti-Citrinin Antibody Based on Mimotope X27
Published in Toxins (13-10-2020)“…Immunoassays are developed based on antigen-antibody interactions. A mimotope is an effective recognition receptor used to study the mechanism of action of…”
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Structure of SARS-CoV-2 main protease in the apo state
Published in Science China. Life sciences (01-04-2021)Get full text
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Isolation and characterisation of deoxynivalenol affinity binders from a phage display library based on single-domain camelid heavy chain antibodies (VHHs)
Published in Food and agricultural immunology (01-06-2012)“…Deoxynivalenol (DON), or vomitoxin, is a mycotoxin of the trichothecene group produced by Fusarium species. The present work described construction of a phage…”
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One-Step Ultrasensitive Bioluminescent Enzyme Immunoassay Based on Nanobody/Nanoluciferase Fusion for Detection of Aflatoxin B 1 in Cereal
Published in Journal of agricultural and food chemistry (08-05-2019)“…Nanoluciferase (Nluc), the smallest luciferase known, was used as the fusion partner with a nanobody against aflatoxin B to develop a bioluminescent enzyme…”
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Landscape of variable domain of heavy‐chain‐only antibody repertoire from alpaca
Published in Immunology (01-09-2020)“…Summary Heavy‐chain‐only antibodies (HCAbs), which are devoid of light chains, have been found naturally occurring in various species including camelids and…”
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Sequence-based design and construction of synthetic nanobody library
Published in Biotechnology and bioengineering (01-06-2024)“…Nanobody (Nb), the smallest antibody fragments known to bind antigens, is now widely applied to various studies, including protein structure analysis,…”
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Single-chain variable fragment antibody-based immunochromatographic strip for rapid detection of fumonisin B1 in maize samples
Published in Food chemistry (30-07-2020)“…[Display omitted] •Single-chain variable fragment antibody-based rapid test strip has been developed.•The immunochromatographic strip (ICS) showed good…”
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One-step orientated immobilization of nanobodies and its application for immunoglobulin purification
Published in Journal of Chromatography A (11-10-2019)“…•The novel IgG affinity ligands are expressed as soluble proteins in E. coli.•No needs of purifying the affinity ligand before immobilization.•The performance…”
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Single-chain variable fragment antibody-based immunochromatographic strip for rapid detection of fumonisin B 1 in maize samples
Published in Food chemistry (30-07-2020)“…A rapid and sensitive immunochromatographic strip (ICS) based on a single-chain variable fragment (scFv) was developed for detecting fumonisin B (FB ). The ICS…”
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Tandem nanobody: A feasible way to improve the capacity of affinity chromatography
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (30-05-2021)“…•Dramatically improving binding capacity by tandem affinity ligand.•No needs of purifying the affinity ligand before immobilization.•The performance of the…”
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Anti-idiotypic nanobody-alkaline phosphatase fusion proteins: Development of a one-step competitive enzyme immunoassay for fumonisin B1 detection in cereal
Published in Analytica chimica acta (14-06-2016)“…A rapid and sensitive one-step competitive enzyme immunoassay for the detection of FB1 was developed. The anti-idiotypic nanobody–alkaline phosphatase…”
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Panning anti-LPS nanobody as a capture target to enrich Vibrio fluvialis
Published in Biochemical and biophysical research communications (07-05-2019)“…Vibrio fluvialis is considered as a human pathogen in developing countries. This bacterium is widely distributed in seawater and harbors that contains traces…”
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Structure-Based Discovery and Structural Basis of a Novel Broad-Spectrum Natural Product against the Main Protease of Coronavirus
Published in Journal of virology (12-01-2022)“…Over the past 20 years, the severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome CoV (MERS-CoV), and SARS-CoV-2 emerged,…”
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A potent neutralizing nanobody targeting a unique epitope on the receptor-binding domain of SARS-CoV-2 spike protein
Published in Virology (New York, N.Y.) (01-01-2024)“…SARS-CoV-2 and its variants continue to threaten public health. Nanobodies that block the attachment of the RBD to host cell angiotensin-converting enzyme 2…”
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The ctnF gene is involved in citrinin and pigment synthesis in Monascus aurantiacus
Published in Journal of basic microbiology (01-10-2020)“…The application of Monascus is restricted by citrinin. So, it is important to explore the synthetic pathway of citrinin to completely inhibit the production of…”
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Citrinin detection using phage-displayed anti-idiotypic single-domain antibody for antigen mimicry
Published in Food chemistry (15-06-2015)“…•The anti-idiotypic single-domain antibody can be straightforward obtained from a naïve alpaca library.•The anti-idiotypic single-domain antibody can serve as…”
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