Pantropic retroviral vectors mediate somatic cell transformation and expression of foreign genes in dipteran insects

Pantropic retroviral vectors mediate somatic cell transformation and expression of foreign genes in dipteran insects

The control of insects that transmit disease and damage crops has become increasingly difficult. The ability to genetically engineer insects would facilitate strategies to protect crops and block arthropod vector-borne disease transmission. Transformation vectors based on insect transposable element...

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Bibliographic Details
Published in:Insect molecular biology Vol. 7; no. 3; pp. 215 - 222
Main Authors: Jordan, T. V., Shike, H., Boulo, V., Cedeno, V., Fang, Q., Davis, B. S., Jacobs‐Lorena, M., Higgs, S., Fryxell1, K. J., Burns, J. C.
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01-08-1998
Subjects:
Aedes - genetics
Animals
Anopheles - genetics
beta-Galactosidase - genetics
Cell Line, Transformed
Diptera - genetics
Diptera - virology
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Drosophila melanogaster - virology
Female
Gene Expression
Genetic Vectors
Larva
Mice
Microinjections
Moloney murine leukemia virus - genetics
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid
Transformation, Genetic
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Summary:The control of insects that transmit disease and damage crops has become increasingly difficult. The ability to genetically engineer insects would facilitate strategies to protect crops and block arthropod vector-borne disease transmission. Transformation vectors based on insect transposable elements have been developed, but most have limited host ranges. A promising alternative is the pantropic retroviral vector, which is packaged with the envelope glycoprotein from vesicular stomatitis virus and is replication-defective. We show here that pantropic murine retroviral vectors can mediate high-level expression of foreign genes in somatically transformed insect larvae and adults of three dipteran genera. This success demonstrates the potential for germline transformation mediated by pantropic retroviral vectors.
Bibliography:istex:57459ABB0A31C486D2338CB60ADADA2F3565B36F
ArticleID:IMB63
ark:/67375/WNG-H48ZVQ27-5
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.1998.00063.x