Biological properties of electrospun cellulose scaffolds from biomass

Nowadays the development of sustainable technologies for the effective production of polymeric materials that can be used as biomaterials will be of importance. In this work, cellulose (CEL) was purified from potato peel waste (PPW) and used to produce electrospun nanofibers for tissue engineering a...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of biomaterials science. Polymer ed. Vol. 30; no. 15; pp. 1399 - 1414
Main Authors:	Friend, Debora Ferreira Laurito, Leyva González, Maria Elena, Caraballo, Mirta Mir, de Queiroz, Alvaro Antonio Alencar
Format:	Journal Article
Language:	English
Published:	Abingdon Taylor & Francis 13-10-2019 Taylor & Francis Ltd
Subjects:	BALB-3T3 Cell adhesion & migration Cellulose scaffolds Tissue engineering
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Nowadays the development of sustainable technologies for the effective production of polymeric materials that can be used as biomaterials will be of importance. In this work, cellulose (CEL) was purified from potato peel waste (PPW) and used to produce electrospun nanofibers for tissue engineering applications. The purified CEL was solubilized in copper ethylenediamine (Cuen) and the electrospun nanofibers was produced through electrospinning technique in diameter range of 250-500 nm at electrical field strength of 20 kV. To confirm the applicability of the electrospun CEL scaffolds in tissue engineering, in vitro BALB-3T3 fibroblastic cell adhesion and cell proliferation tests were employed in this study. Cell viability was evaluated by staining with ethidium bromide (EtBr) and acridine orange (AO) to evaluate the possible effects of cytotoxicity of the CNF scaffolds. Fluorescence studies confirmed that BALB-3T3 viable cells attached and spread throughout the CEL scaffold. The attachment and spreading of viable cells suggests that electrospun CEL scaffolds support growth of BALB-3T3 fibroblasts cells and suggests that PPW can be a useful source of raw material for the production of scaffolds for tissue engineering.
ISSN:	0920-5063 1568-5624
DOI:	10.1080/09205063.2019.1636351