Search Results - "Friedman, Larry"

Mechanism of Transcription Initiation at an Activator-Dependent Promoter Defined by Single-Molecule Observation by Friedman, Larry J., Gelles, Jeff

“…Understanding the pathway and kinetic mechanisms of transcription initiation is essential for quantitative understanding of gene regulation, but initiation is…”
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Multi-wavelength single-molecule fluorescence analysis of transcription mechanisms by Friedman, Larry J., Gelles, Jeff

“…•Methods for elucidation of transcription mechanisms by single-molecule fluorescence.•Processing of multi-wavelength single-molecule co-localization…”
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Single-molecule studies of origin licensing reveal mechanisms ensuring bidirectional helicase loading by Ticau, Simina, Friedman, Larry J, Ivica, Nikola A, Gelles, Jeff, Bell, Stephen P

“…Loading of the ring-shaped Mcm2-7 replicative helicase around DNA licenses eukaryotic origins of replication. During loading, Cdc6, Cdt1, and the…”
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Single-molecule studies reveal branched pathways for activator-dependent assembly of RNA polymerase II pre-initiation complexes by Baek, Inwha, Friedman, Larry J., Gelles, Jeff, Buratowski, Stephen

“…RNA polymerase II (RNA Pol II) transcription reconstituted from purified factors suggests pre-initiation complexes (PICs) can assemble by sequential…”
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RNA polymerase sliding on DNA can couple the transcription of nearby bacterial operons by Tenenbaum, Debora, Inlow, Koe, Friedman, Larry J, Cai, Anthony, Gelles, Jeff, Kondev, Jane

“…DNA transcription initiates after an RNA polymerase (RNAP) molecule binds to the promoter of a gene. In bacteria, the canonical picture is that RNAP comes from…”
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Recycling of bacterial RNA polymerase by the Swi2/Snf2 ATPase RapA by Inlow, Koe, Tenenbaum, Debora, Friedman, Larry J, Kondev, Jane, Gelles, Jeff

“…Free-living bacteria have regulatory systems that can quickly reprogram gene transcription in response to changes in the cellular environment. The RapA ATPase,…”
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Changing protein-DNA interactions promote ORC binding-site exchange during replication origin licensing by Zhang, Annie, Friedman, Larry J, Gelles, Jeff, Bell, Stephen P

“…During origin licensing, the eukaryotic replicative helicase Mcm2-7 forms head-to-head double hexamers to prime origins for bidirectional replication. Recent…”
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Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription by Rosen, Grace A., Baek, Inwha, Friedman, Larry J., Joo, Yoo Jin, Buratowski, Stephen, Gelles, Jeff

“…In eukaryotes, RNA polymerase II (RNApII) transcribes messenger RNA from template DNA. Decades of experiments have identified the proteins needed for…”
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A helicase-tethered ORC flip enables bidirectional helicase loading by Gupta, Shalini, Friedman, Larry J, Gelles, Jeff, Bell, Stephen P

“…Replication origins are licensed by loading two Mcm2-7 helicases around DNA in a head-to-head conformation poised to initiate bidirectional replication. This…”
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Regulation of replication origin licensing by ORC phosphorylation reveals a two-step mechanism for Mcm2-7 ring closing by Amasino, Audra L, Gupta, Shalini, Friedman, Larry J, Gelles, Jeff, Bell, Stephen P

“…Eukaryotic DNA replication must occur exactly once per cell cycle to maintain cell ploidy. This outcome is ensured by temporally separating replicative…”
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Ordered and Dynamic Assembly of Single Spliceosomes by Hoskins, Aaron A, Friedman, Larry J, Gallagher, Sarah S, Crawford, Daniel J, Anderson, Eric G, Wombacher, Richard, Ramirez, Nicholas, Cornish, Virginia W, Gelles, Jeff, Moore, Melissa J

“…The spliceosome is the complex macromolecular machine responsible for removing introns from precursors to messenger RNAs (pre-mRNAs). We combined yeast genetic…”
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Mechanism and timing of Mcm2–7 ring closure during DNA replication origin licensing by Ticau, Simina, Friedman, Larry J, Champasa, Kanokwan, Corrêa, Ivan R, Gelles, Jeff, Bell, Stephen P

“…smFRET analysis and colocalization spectroscopy reveal the mechanism of DNA entry into the Mcm2–7 helicase ring during replication origin licensing. The…”
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The endoplasmic reticulum chaperone BiP is a closure-accelerating cochaperone of Grp94 by Huang, Bin, Sun, Ming, Hoxie, Reyal, Kotler, Judy L M, Friedman, Larry J, Gelles, Jeff, Street, Timothy O

“…Hsp70 and Hsp90 chaperones provide protein quality control to the cytoplasm, endoplasmic reticulum (ER), and mitochondria. Hsp90 activity is often enhanced by…”
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RNA polymerase approaches its promoter without long-range sliding along DNA by Friedman, Larry J., Mumm, Jeffrey P., Gelles, Jeff

“…Sequence-specific DNA binding proteins must quickly bind target sequences, despite the enormously larger amount of nontarget DNA present in cells. RNA…”
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Conformational Cycling within the Closed State of Grp94, an Hsp90-Family Chaperone by Huang, Bin, Friedman, Larry J., Sun, Ming, Gelles, Jeff, Street, Timothy O.

“…The Hsp90 family of chaperones requires ATP-driven cycling to perform their function. The presence of two bound ATP molecules is known to favor a closed…”
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A general mechanism for competitor-induced dissociation of molecular complexes by Paramanathan, Thayaparan, Reeves, Daniel, Friedman, Larry J., Kondev, Jane, Gelles, Jeff

“…The kinetic stability of non-covalent macromolecular complexes controls many biological phenomena. Here we find that physical models of complex dissociation…”
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The dimerization equilibrium of a ClC Cl(-)/H(+) antiporter in lipid bilayers by Chadda, Rahul, Krishnamani, Venkatramanan, Mersch, Kacey, Wong, Jason, Brimberry, Marley, Chadda, Ankita, Kolmakova-Partensky, Ludmila, Friedman, Larry J, Gelles, Jeff, Robertson, Janice L

“…Interactions between membrane protein interfaces in lipid bilayers play an important role in membrane protein folding but quantification of the strength of…”
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A conserved Mcm4 motif is required for Mcm2-7 double-hexamer formation and origin DNA unwinding by Champasa, Kanokwan, Blank, Caitlin, Friedman, Larry J, Gelles, Jeff, Bell, Stephen P

“…Licensing of eukaryotic origins of replication requires DNA loading of two copies of the Mcm2-7 replicative helicase to form a head-to-head double-hexamer,…”
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7 by De Jesús-Kim, Lorraine, Friedman, Larry J, Lõoke, Marko, Ramsoomair, Christian K, Gelles, Jeff, Bell, Stephen P

“…The committed step of eukaryotic DNA replication occurs when the pairs of Mcm2-7 replicative helicases that license each replication origin are activated…”
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Dynamics of GreB-RNA polymerase interaction allow a proofreading accessory protein to patrol for transcription complexes needing rescue by Tetone, Larry E., Friedman, Larry J., Osborne, Melisa L., Ravi, Harini, Kyzer, Scotty, Stumper, Sarah K., Mooney, Rachel A., Landick, Robert, Gelles, Jeff

“…The secondary channel (SC) of multisubunit RNA polymerases (RNAPs) allows access to the active site and is a nexus for the regulation of transcription…”
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