Search Results - "Frey, P A"

Radical mechanisms of enzymatic catalysis by Frey, P A

“…Two classes of enzymatic mechanisms that proceed by free radical chemistry initiated by the 5'-deoxyadenosyl radical are discussed. In the first class, the…”
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Enantiomeric Free Radicals and Enzymatic Control of Stereochemistry in a Radical Mechanism:  The Case of Lysine 2,3-Aminomutases by Behshad, E, Ruzicka, F. J, Mansoorabadi, S. O, Chen, D, Reed, G. H, Frey, P. A

“…The product of yjeK in Escherichia coli is a homologue of lysine 2,3-aminomutase (LAM) from Clostridium subterminale SB4, and both enzymes catalyze the…”
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A Low-Barrier Hydrogen Bond in the Catalytic Triad of Serine Proteases by Frey, Perry A., Whitt, Sean A., Tobin, John B.

“…Spectroscopic properties of chymotrypsin and model compounds indicate that a low-barrier hydrogen bond participates in the mechanism of serine protease action…”
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Characterization of an Allylic Analogue of the 5‘-Deoxyadenosyl Radical: An Intermediate in the Reaction of Lysine 2,3-Aminomutase by Magnusson, Olafur Th, Reed, George H, Frey, Perry A

“…An allylic analogue of the 5‘-deoxyadenosyl radical has been characterized at the active site of lysine 2,3-aminomutase (LAM) by electron paramagnetic…”
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Direct FeS Cluster Involvement in Generation of a Radical in Lysine 2,3-Aminomutase by Cosper, Nathaniel J, Booker, Squire J, Ruzicka, Frank, Frey, Perry A, Scott, Robert A

“…Lysine 2,3-aminomutase (KAM) belongs to a class of enzymes that use FeS clusters and S-adenosyl-l-methionine to initiate radical-dependent chemistry. Selenium…”
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Enzymology. Coenzymes and radicals by Frey, P A

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Mechanistic Roles of Thr134, Tyr160, and Lys 164 in the Reaction Catalyzed by dTDP-Glucose 4,6-Dehydratase by Gerratana, Barbara, Cleland, W. W, Frey, Perry A

“…Escherichia coli dTDP-glucose 4,6-dehydratase and UDP-galactose 4-epimerase are members of the short-chain dehydrogenase/reductase SDR family. A highly…”
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Correlation of Low-Barrier Hydrogen Bonding and Oxyanion Binding in Transition State Analogue Complexes of Chymotrypsin by Neidhart, David, Wei, Yaoming, Cassidy, Constance, Lin, Jing, Cleland, W. Wallace, Frey, Perry A

“…The structures of the hemiketal adducts of Ser 195 in chymotrypsin with N-acetyl-l-leucyl-l-phenylalanyl trifluoromethyl ketone (AcLF-CF3) and…”
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Characterization of Enzymatic Processes by Rapid Mix−Quench Mass Spectrometry:  The Case of dTDP-glucose 4,6-Dehydratase by Gross, Jeffrey W, Hegeman, Adrian D, Vestling, Martha M, Frey, Perry A

“…The single-turnover kinetic mechanism for the reaction catalyzed by dTDP-glucose 4,6-dehydratase (4,6-dehydratase) has been determined by rapid mix−chemical…”
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Lysine 2,3-Aminomutase and trans-4,5-Dehydrolysine:  Characterization of an Allylic Analogue of a Substrate-Based Radical in the Catalytic Mechanism by Wu, Weiming, Booker, Squire, Lieder, Kafryn W, Bandarian, Vahe, Reed, George H, Frey, Perry A

“…An analogue of lysine, trans-4,5-dehydro-l-lysine (trans-4,5-dehydrolysine), is a potent inhibitor of lysine 2,3-aminomutase from Clostridium subterminale SB4…”
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Electron Transfer in the Substrate-Dependent Suicide Inactivation of Lysine 5,6-Aminomutase by Tang, Kuo-Hsiang, Chang, Christopher H, Frey, Perry A

“…The lysine 5,6-aminomutase (5,6-LAM) purified from Clostridium sticklandii was found to undergo rapid inactivation in the absence of the activating enzyme E2…”
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Identification of Lysine 346 as a Functionally Important Residue for Pyridoxal 5‘-Phosphate Binding and Catalysis in Lysine 2,3-Aminomutase from Bacillus subtilis by Chen, Dawei, Frey, Perry A

“…Lysine 2,3-aminomutase (LAM) catalyzes the interconversion of l-lysine and l-β-lysine. The enzyme contains pyridoxal 5‘-phosphate (PLP) and a [4Fe-4S] center…”
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High‐resolution X‐ray structure of UDP‐galactose 4‐epimerase complexed with UDP‐phenol by Thoden, James B., Frey, Perry A., Holden, Hazel M.

“…UDP‐galactose 4‐epimerase from Escherichia coli catalyzes the interconversion of UDP‐glucose and UDP‐galactose. In recent years, the enzyme has been the…”
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The role of S-adenosylmethionine in the lysine 2,3-aminomutase reaction by Moss, M, Frey, P A

“…The interconversion of L-lysine and L-3,6-diamino-hexanoate (L-beta-lysine) catalyzed by lysine 2,3-aminomutase is known to be stimulated by added…”
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Inhibition of Lysine 2,3-Aminomutase by the Alternative Substrate 4-Thialysine and Characterization of the 4-Thialysyl Radical Intermediate by Miller, Jennifer, Bandarian, Vahe, Reed, George H., Frey, Perry A.

“…Lysine 2,3-aminomutase catalyzes the interconversion of l-lysine and l-β-lysine. 4-Thia-l-lysine (4-thialysine) is an alternative substrate for Lysine…”
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Dehydration Is Catalyzed by Glutamate-136 and Aspartic Acid-135 Active Site Residues in Escherichia coli dTDP-Glucose 4,6-Dehydratase by Gross, Jeffrey W, Hegeman, Adrian D, Gerratana, Barbara, Frey, Perry A

“…The dTDP-glucose 4,6-dehydratase catalyzed conversion of dTDP-glucose to dTDP-4-keto-6-deoxyglucose occurs in three sequential chemical steps: …”
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Characterization of the Transition-State Structure of the Reaction of Kanamycin Nucleotidyltransferase by Heavy-Atom Kinetic Isotope Effects by Gerratana, Barbara, Frey, Perry A, Cleland, W. W

“…The transition-state structure for the reaction catalyzed by kanamycin nucleotidyltransferase has been determined from kinetic isotope effects. The primary 18O…”
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Lysine 2,3-aminomutase. Support for a mechanism of hydrogen transfer involving S-adenosylmethionine by BARANIAK, J, MOSS, M. L, FREY, P. A

“…The conversion of L-lysine to L-beta-lysine is catalyzed by lysine 2,3-aminomutase. The reaction involves the interchange of the 2-amino group of lysine with a…”
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The Low Barrier Hydrogen Bond in Enzymatic Catalysis by Cleland, W. Wallace, Frey, Perry A., Gerlt, John A.

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The Leloir pathway: a mechanistic imperative for three enzymes to change the stereochemical configuration of a single carbon in galactose by Frey, P A

“…The biological interconversion of galactose and glucose takes place only by way of the Leloir pathway and requires the three enzymes galactokinase,…”
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