Transgenic tomato expressing an oxalate decarboxylase gene from Flammulina sp. shows increased survival to Moniliophthora perniciosa

Transgenic tomato expressing an oxalate decarboxylase gene from Flammulina sp. shows increased survival to Moniliophthora perniciosa

•oxdc gene from flammulina velutipes was used to generate transgenic tomato plants.•Transgenic T1 lines showed a significant delay in symptom development.•Transgenic T1 lines showed a higher survival percentage to witches’ broom disease.•The P16 line showed a decreased production of H2O2 in the pres...

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Bibliographic Details
Published in:Scientia horticulturae Vol. 299; p. 111004
Main Authors: Pereira Menezes Reis, Sara, de Andrade Silva, Edson Mario, Peres Gramacho, Karina, Freitas Sena, Kaleandra, da Costa Silva, Delmira, Lima Aragão, Francisco José, Cardoso Costa, Marcio Gilberto, Micheli, Fabienne
Format: Journal Article
Language:English
Published: Elsevier B.V 01-06-2022
Subjects:
Disease resistance
Oxalic acid
Plant-pathogen interaction
Witches’ broom disease
Plant-pathogen interaction
Disease resistance
OA
Oxalic acid
DAB
COC
OXO
ROS
Witches’ broom disease
dai
GAPDH
WT
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Summary:•oxdc gene from flammulina velutipes was used to generate transgenic tomato plants.•Transgenic T1 lines showed a significant delay in symptom development.•Transgenic T1 lines showed a higher survival percentage to witches’ broom disease.•The P16 line showed a decreased production of H2O2 in the presence of oxalic acid.•Witches’ broom disease progression was reduced by the presence of the oxdc gene. Witches’ broom disease which affects cacao tree (Theobroma cacao L.) is caused by the hemibiotrophic fungus Moniliophthora perniciosa. Studies of the cacao-M. perniciosa interaction showed that the pathogenicity of M. perniciosa is associated to the presence of oxalic acid (OA). In some organisms, OA can be degraded into formate and CO2 by the oxalate decarboxylase (OXDC) enzyme. Here, we used the oxdc gene from Flammulina velutipes to generate transgenic tomato plants as a potential strategy to control M. perniciosa infection. Three transgenic T1 lines expressing the oxdc gene were obtained and two of them (P4 and P16) presented a significant delay in symptom development and higher survival percentage when inoculated with M. perniciosa in comparison to the non-transformed wild-type (WT) plants. Moreover, the P16 line test showed a decreased production of H2O2 in the presence of OA in comparison to the WT, as evidenced by the 3,3′-diaminobenzidine test. These results indicate that the P16 transgenic line is able to break down OA leading to normal plant development with few symptoms even when infected by M. perniciosa. Phenotypic and histological analysis showed differences between WT and transgenic plants. The amount of fungus hyphae was lower with thinner thickness in the transgenic plants. This study evidences of the reduction of witches’ broom disease progression by the presence of the oxdc gene leading to the plant survival.
ISSN:0304-4238
1879-1018
DOI:10.1016/j.scienta.2022.111004