Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application

Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate...

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Published in:	BMC biotechnology Vol. 19; no. 1; pp. 79 - 14
Main Authors:	Freitas, Elinea O, Melo, Bruno P, Lourenço-Tessutti, Isabela T, Arraes, Fabrício B M, Amorim, Regina M, Lisei-de-Sá, Maria E, Costa, Julia A, Leite, Ana G B, Faheem, Muhammad, Ferreira, Márcio A, Morgante, Carolina V, Fontes, Elizabeth P B, Grossi-de-Sa, Maria F
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 20-11-2019 BioMed Central BMC
Subjects:	Abiotic stress Abscisic acid Abscisic Acid - pharmacology Agricultural industry Agronomy Amino acids Arabidopsis thaliana Biochemistry Biotechnology Biotechnology - methods Cultivars Displays (Marketing) Drought Drought resistance Drought tolerance Droughts Economic importance Gene expression Gene Expression Regulation, Plant - drug effects Gene Expression Regulation, Plant - genetics Gene sequencing Gene-promoter characterization Genes Genetic research Genomes Genomics Glycine max - drug effects Glycine max - genetics Glycols (Class of compounds) Lead Modules Phylogenetics Physiological aspects Physiological effects Physiology Plant hormones Polyethylene glycol Polyols Production management Promoter modules analysis Promoter Regions, Genetic - drug effects Promoter Regions, Genetic - genetics Promoters (Genetics) Reporter gene Salinity Senescence Signal transduction Soybeans Stress response Stress, Physiological - genetics Stress, Physiological - physiology Stress-responsive promoter Stresses Transcription Transcription factors Water deficit Water deprivation Abscisic acid Stress-responsive promoter Promoter modules analysis Drought tolerance Gene-promoter characterization
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Summary:	Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses.
ISSN:	1472-6750 1472-6750
DOI:	10.1186/s12896-019-0561-3