Search Results - "Freeman, Alasdair D. J."

Single-Molecule Observation of the Induction of k-Turn RNA Structure on Binding L7Ae Protein by Wang, Jia, Fessl, Tomáš, Schroeder, Kersten T., Ouellet, Jonathan, Liu, Yijin, Freeman, Alasdair D.J., Lilley, David M.J.

“…The k-turn is a commonly occurring structural motif that introduces a tight kink into duplex RNA. In free solution, it can exist in an extended form, or by…”
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Junction resolving enzymes use multivalency to keep the Holliday junction dynamic by Zhou, Ruobo, Yang, Olivia, Déclais, Anne-Cécile, Jin, Hyeonseok, Gwon, Gwang Hyeon, Freeman, Alasdair D. J., Cho, Yunje, Lilley, David M. J., Ha, Taekjip

“…Holliday junction (HJ) resolution by resolving enzymes is essential for chromosome segregation and recombination-mediated DNA repair. HJs undergo two types of…”
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Observing Spontaneous Branch Migration of Holliday Junctions One Step at a Time by McKinney, Sean A., Alasdair D. J. Freeman, David M. J. Lilley, Ha, Taekjip, Mizuuchi, Kiyoshi

“…Genetic recombination occurs between homologous DNA molecules via a four-way (Holliday) junction intermediate. This ancient and ubiquitous process is important…”
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Analysis of Conformational Changes in the DNA Junction-Resolving Enzyme T7 Endonuclease I on Binding a Four-Way Junction Using EPR by Freeman, Alasdair D. J, Ward, Richard, El Mkami, Hassane, Lilley, David M. J, Norman, David G

“…The four-way (Holliday) DNA junction is the central intermediate in homologous recombination. It is ultimately resolved into two nicked-duplex species by the…”
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A monovalent ion in the DNA binding interface of the eukaryotic junction-resolving enzyme GEN1 by Liu, Yijin, Freeman, Alasdair DJ, Déclais, Anne-Cécile, Lilley, David M J

“…Abstract GEN1 is a member of the FEN/EXO family of structure-selective nucleases that cleave 1 nt 3′ to a variety of branchpoints. For each, the H2TH motif…”
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Analysis of the Intrinsically Disordered N‑Terminus of the DNA Junction-Resolving Enzyme T7 Endonuclease I: Identification of Structure Formed upon DNA Binding by Freeman, Alasdair D. J, Stevens, Michael, Declais, Anne-Cecile, Leahy, Adam, Mackay, Katherine, El Mkami, Hassane, Lilley, David M. J, Norman, David G

“…The four-way (Holliday) DNA junction of homologous recombination is processed by the symmetrical cleavage of two strands by a nuclease. These…”
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GEN1 from a Thermophilic Fungus Is Functionally Closely Similar to Non-Eukaryotic Junction-Resolving Enzymes by Freeman, Alasdair D.J., Liu, Yijin, Déclais, Anne-Cécile, Gartner, Anton, Lilley, David M.J.

“…Processing of Holliday junctions is essential in recombination. We have identified the gene for the junction-resolving enzyme GEN1 from the thermophilic fungus…”
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The Importance of the N-Terminus of T7 Endonuclease I in the Interaction with DNA Junctions by Freeman, Alasdair D.J., Déclais, Anne-Cécile, Lilley, David M.J.

“…T7 endonuclease I is a dimeric nuclease that is selective for four-way DNA junctions. Previous crystallographic studies have found that the N-terminal 16 amino…”
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Human ANKLE1 Is a Nuclease Specific for Branched DNA by Song, Junfang, Freeman, Alasdair D.J., Knebel, Axel, Gartner, Anton, Lilley, David M.J.

“…All physical connections between sister chromatids must be broken before cells can divide, and eukaryotic cells have evolved multiple ways in which to process…”
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Biochemical and mechanistic analysis of the cleavage of branched DNA by human ANKLE1 by Freeman, Alasdair D J, Déclais, Anne-Cécile, Wilson, Timothy J, Lilley, David M J

“…Abstract ANKLE1 is a nuclease that provides a final opportunity to process unresolved junctions in DNA that would otherwise create chromosomal linkages…”
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The complex between a four-way DNA junction and T7 endonuclease I by Déclais, Anne-Cécile, Fogg, Jonathan M., Freeman, Alasdair D. J., Coste, Franck, Hadden, Jonathan M., Phillips, Simon E. V., Lilley, David M. J.

“…The junction‐resolving enzyme endonuclease I is selective for the structure of the DNA four‐way (Holliday) junction. The enzyme binds to a four‐way junction in…”
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Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to DNA by Liu, Yijin, Freeman, Alasdair D.J., Déclais, Anne-Cécile, Wilson, Timothy J., Gartner, Anton, Lilley, David M.J.

“…We present the crystal structure of the junction-resolving enzyme GEN1 bound to DNA at 2.5 Å resolution. The structure of the GEN1 protein reveals it to have…”
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Structural Recognition between a Four-way DNA Junction and a Resolving Enzyme by Déclais, Anne-Cécile, Liu, Jia, Freeman, Alasdair D.J., Lilley, David M.J.

“…Resolving enzymes bind highly selectively to four-way DNA junctions, but the mechanism of this structural specificity is poorly understood. In this study, we…”
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Metal Ion Binding in the Active Site of the Junction-resolving Enzyme T7 Endonuclease I in the Presence and in the Absence of DNA by Freeman, Alasdair D.J, Déclais, Anne-Cécile, Lilley, David M.J

“…Endonuclease I of bacteriophage T7 is a DNA junction-resolving enzyme. We have previously used crystallography to demonstrate the binding of two manganese ions…”
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Investigation of the DNA binding domain of nuclear factor I by Freeman, Alasdair D J

“…Nuclear Factor I is a cellular transcription factor involved in the initiation of adenovirus type 2 replication. It exists as a family of proteins and…”
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