Search Results - "Fredrick, Kurt"

Intersubunit Bridges of the Bacterial Ribosome by Liu, Qi, Fredrick, Kurt

“…The ribosome is a large two-subunit ribonucleoprotein machine that translates the genetic code in all cells, synthesizing proteins according to the sequence of…”
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How the Sequence of a Gene Can Tune Its Translation by Fredrick, Kurt, Ibba, Michael

“…Sixty-one codons specify 20 amino acids, offering cells many options for encoding a polypeptide sequence. Two new studies ( Cannarrozzi et al., 2010; Tuller et…”
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Cryptic and abundant marine viruses at the evolutionary origins of Earth's RNA virome by Zayed, Ahmed A, Wainaina, James M, Dominguez-Huerta, Guillermo, Pelletier, Eric, Guo, Jiarong, Mohssen, Mohamed, Tian, Funing, Pratama, Akbar Adjie, Bolduc, Benjamin, Zablocki, Olivier, Cronin, Dylan, Solden, Lindsey, Delage, Erwan, Alberti, Adriana, Aury, Jean-Marc, Carradec, Quentin, da Silva, Corinne, Labadie, Karine, Poulain, Julie, Ruscheweyh, Hans-Joachim, Salazar, Guillem, Shatoff, Elan, Bundschuh, Ralf, Fredrick, Kurt, Kubatko, Laura S, Chaffron, Samuel, Culley, Alexander I, Sunagawa, Shinichi, Kuhn, Jens H, Wincker, Patrick, Sullivan, Matthew B, Acinas, Silvia G, Babin, Marcel, Bork, Peer, Boss, Emmanuel, Bowler, Chris, Cochrane, Guy, de Vargas, Colomban, Gorsky, Gabriel, Guidi, Lionel, Grimsley, Nigel, Hingamp, Pascal, Iudicone, Daniele, Jaillon, Olivier, Kandels, Stefanie, Karp-Boss, Lee, Karsenti, Eric, Not, Fabrice, Ogata, Hiroyuki, Poulton, Nicole, Pesant, Stéphane, Sardet, Christian, Speich, Sabrinia, Stemmann, Lars, Sullivan, Matthew B, Sungawa, Shinichi, Wincker, Patrick

“…Whereas DNA viruses are known to be abundant, diverse, and commonly key ecosystem players, RNA viruses are insufficiently studied outside disease settings. In…”
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Roles of helix H69 of 23S rRNA in translation initiation by Liu, Qi, Fredrick, Kurt

“…Initiation of translation involves the assembly of a ribosome complex with initiator tRNA bound to the peptidyl site and paired to the start codon of the mRNA…”
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Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth by Dai, Xiongfeng, Zhu, Manlu, Warren, Mya, Balakrishnan, Rohan, Patsalo, Vadim, Okano, Hiroyuki, Williamson, James R., Fredrick, Kurt, Wang, Yi-Ping, Hwa, Terence

“…Bacteria growing under different conditions experience a broad range of demand on the rate of protein synthesis, which profoundly affects cellular resource…”
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In-cell SHAPE reveals that free 30S ribosome subunits are in the inactive state by McGinnis, Jennifer L., Liu, Qi, Lavender, Christopher A., Devaraj, Aishwarya, McClory, Sean P., Fredrick, Kurt, Weeks, Kevin M.

“…It was shown decades ago that purified 30S ribosome subunits readily interconvert between “active” and “inactive” conformations in a switch that involves…”
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Preparation and evaluation of acylated tRNAs by Walker, Sarah E., Fredrick, Kurt

“…In the cell, the activity of tRNA is governed by its acylation state. Interactions with the ribosome, translation factors, and regulatory elements are strongly…”
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Ribosomal Translocation: One Step Closer to the Molecular Mechanism by Shoji, Shinichiro, Walker, Sarah E, Fredrick, Kurt

“…Protein synthesis occurs in ribosomes, the targets of numerous antibiotics. How these large and complex machines read and move along mRNA have proven to be…”
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Role of helix 44 of 16S rRNA in the fidelity of translation initiation by Qin, Daoming, Liu, Qi, Devaraj, Aishwarya, Fredrick, Kurt

“…The molecular mechanisms that govern translation initiation to ensure accuracy remain unclear. Here, we provide evidence that the subunit-joining step of…”
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Reverse Translocation of tRNA in the Ribosome by Shoji, Shinichiro, Walker, Sarah E., Fredrick, Kurt

“…A widely held view is that directional movement of tRNA in the ribosome is determined by an intrinsic mechanism and driven thermodynamically by…”
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BipA exerts temperature-dependent translational control of biofilm-associated colony morphology in Vibrio cholerae by Del Peso Santos, Teresa, Alvarez, Laura, Sit, Brandon, Irazoki, Oihane, Blake, Jonathon, Warner, Benjamin R, Warr, Alyson R, Bala, Anju, Benes, Vladimir, Waldor, Matthew K, Fredrick, Kurt, Cava, Felipe

“…Adaptation to shifting temperatures is crucial for the survival of the bacterial pathogen . Here, we show that colony rugosity, a biofilm-associated phenotype,…”
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Direct measurement of the mechanical work during translocation by the ribosome by Liu, Tingting, Kaplan, Ariel, Alexander, Lisa, Yan, Shannon, Wen, Jin-Der, Lancaster, Laura, Wickersham, Charles E, Fredrick, Kurt, Fredrik, Kurt, Noller, Harry, Tinoco, Ignacio, Bustamante, Carlos J

“…A detailed understanding of tRNA/mRNA translocation requires measurement of the forces generated by the ribosome during this movement. Such measurements have…”
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Structural basis for hygromycin B inhibition of protein biosynthesis by Borovinskaya, Maria A, Shoji, Shinichiro, Fredrick, Kurt, Cate, Jamie H D

“…Aminoglycosides are one of the most widely used and clinically important classes of antibiotics that target the ribosome. Hygromycin B is an atypical…”
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Role of hybrid tRNA-binding states in ribosomal translocation by Walker, Sarah E, Shoji, Shinichiro, Pan, Dongli, Cooperman, Barry S, Fredrick, Kurt

“…During translation, tRNAs must move rapidly to their adjacent sites in the ribosome while maintaining precise pairing with mRNA. This movement (translocation)…”
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Resampling and Editing of Mischarged tRNA Prior to Translation Elongation by Ling, Jiqiang, So, Byung Ran, Yadavalli, Srujana S., Roy, Hervé, Shoji, Shinichiro, Fredrick, Kurt, Musier-Forsyth, Karin, Ibba, Michael

“…Faithful translation of the genetic code depends on the GTPase EF-Tu delivering correctly charged aminoacyl-tRNAs to the ribosome for pairing with cognate…”
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Reorganization of an intersubunit bridge induced by disparate 16S ribosomal ambiguity mutations mimics an EF-Tu-bound state by Fagan, Crystal E., Dunkle, Jack A., Maehigashi, Tatsuya, Dang, Mai N., Devaraj, Aishwarya, Miles, Stacey J., Qin, Daoming, Fredrick, Kurt, Dunham, Christine M.

“…After four decades of research aimed at understanding tRNA selection on the ribosome, the mechanism by which ribosomal ambiguity (ram) mutations promote…”
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Modified Aminoglycosides Bind Nucleic Acids in High-Molecular-Weight Complexes by Ying, Lanqing, Zhu, Hongkun, Fosso, Marina Y, Garneau-Tsodikova, Sylvie, Fredrick, Kurt

“…Aminoglycosides represent a large group of antibiotics well known for their ability to target the bacterial ribosome. In studying 6"-substituted variants of…”
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Comparative Analysis of anti-Shine- Dalgarno Function in Flavobacterium johnsoniae and Escherichia coli by McNutt, Zakkary A, Gandhi, Mai D, Shatoff, Elan A, Roy, Bappaditya, Devaraj, Aishwarya, Bundschuh, Ralf, Fredrick, Kurt

“…The anti-Shine-Dalgarno (ASD) sequence of 16S rRNA is highly conserved across Bacteria, and yet usage of Shine-Dalgarno (SD) sequences in mRNA varies…”
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Catalysis of Ribosomal Translocation by Sparsomycin by Fredrick, Kurt, Noller, Harry F.

“…During protein synthesis, transfer RNAs (tRNAs) are translocated from the aminoacyl to peptidyl to exit sites of the ribosome, coupled to the movement of…”
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A Steric Block in Translation Caused by the Antibiotic Spectinomycin by Borovinskaya, Maria A, Shoji, Shinichiro, Holton, James M, Fredrick, Kurt, Cate, Jamie H. D

“…The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome…”
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