A Mouse Model to Investigate the Role of Cancer-associated Fibroblasts in Tumor Growth

Cancer-associated fibroblasts (CAFs) can play an important role in tumor growth by creating a tumor-promoting microenvironment. Models to study the role of CAFs in the tumor microenvironment can be helpful for understanding the functional importance of fibroblasts, fibroblasts from different tissues...

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Bibliographic Details
Published in:	Journal of visualized experiments no. 166
Main Authors:	Jelinek, David, Zhang, Ellen Ran, Ambrus, Aaron, Haley, Erin, Guinn, Emily, Vo, Austin, Le, Peter, Kesaf, Ayse Elif, Nguyen, Jennifer, Guo, Lily, Frederick, Destiny, Sun, Zhengyang, Guo, Natalie, Sevier, Parker, Bilotta, Eric, Atai, Kaiser, Voisin, Laurent, Coller, Hilary A
Format:	Journal Article
Language:	English
Published:	United States 22-12-2020
Subjects:	Animals Cancer-Associated Fibroblasts - pathology Cell Line, Tumor Cell Proliferation Disease Models, Animal Humans Injections Melanoma - pathology Mice Neoplasms - pathology Tumor Burden Tumor Microenvironment
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Summary:	Cancer-associated fibroblasts (CAFs) can play an important role in tumor growth by creating a tumor-promoting microenvironment. Models to study the role of CAFs in the tumor microenvironment can be helpful for understanding the functional importance of fibroblasts, fibroblasts from different tissues, and specific genetic factors in fibroblasts. Mouse models are essential for understanding the contributors to tumor growth and progression in an in vivo context. Here, a protocol in which cancer cells are mixed with fibroblasts and introduced into mice to develop tumors is provided. Tumor sizes over time and final tumor weights are determined and compared among groups. The protocol described can provide more insight into the functional role of CAFs in tumor growth and progression.
ISSN:	1940-087X
DOI:	10.3791/61883