Osteogenesis-inducing calcium phosphate nanoparticle precursors applied to titanium surfaces

Osteogenesis-inducing calcium phosphate nanoparticle precursors applied to titanium surfaces

This study investigated the effects of the morphology and physicochemical properties of calcium phosphate (CaP) nanoparticles on osteogenesis. Two types of CaP nanoparticles were compared, namely amorphous calcium phosphate (ACP) nano-spheres (diameter: 9-13 nm) and poorly crystalline apatite (PCA)...

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Bibliographic Details
Published in:Biomedical materials (Bristol) Vol. 8; no. 3; p. 035007
Main Authors: He, Wenxiao, Andersson, Martin, de Souza, Pedro Paulo Chaves, de Souza Costa, Carlos Alberto, Muñoz, Eduardo Mariscal, Schwartz-Filho, Humberto Osvaldo, Hayashi, Mariko, Hemdal, Amanda, Fredel, Axel, Wennerberg, Ann, Jimbo, Ryo
Format: Journal Article
Language:English
Published: England 01-06-2013
Subjects:
Animals
Apatites - chemistry
Bone Substitutes - chemistry
Calcium Phosphates - chemistry
Cells, Cultured
Coated Materials, Biocompatible - chemistry
Gene Expression
Materials Testing
Mice
Microscopy, Electron, Scanning
Nanoparticles - chemistry
Osteoblasts - cytology
Osteoblasts - metabolism
Osteogenesis
Osteotomy
Rabbits
Titanium
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Summary:This study investigated the effects of the morphology and physicochemical properties of calcium phosphate (CaP) nanoparticles on osteogenesis. Two types of CaP nanoparticles were compared, namely amorphous calcium phosphate (ACP) nano-spheres (diameter: 9-13 nm) and poorly crystalline apatite (PCA) nano-needles (30-50 nm × 2-4 nm) that closely resemble bone apatite. CaP particles were spin-coated onto titanium discs and implants; they were evaluated in cultured mouse calvarial osteoblasts, as well as after implantation in rabbit femurs. A significant dependence of CaP coatings was observed in osteoblast-related gene expression (Runx2, Col1a1 and Spp1). Specifically, the PCA group presented an up-regulation of the osteospecific genes, while the ACP group suppressed the Runx2 and Col1a1 expression when compared to blank titanium substrates. Both the ACP and PCA groups presented a more than three-fold increase of calcium deposition, as suggested by Alizarin red staining. The removal torque results implied a slight tendency in favour of the PCA group. Different forms of CaP nanostructures presented different biologic differences; the obtained information can be used to optimize surface coatings on biomaterials.
ISSN:1748-605X
DOI:10.1088/1748-6041/8/3/035007