Search Results - "Frasch, Wayne D."

Protonation-dependent stepped rotation of the F-type ATP synthase c-ring observed by single-molecule measurements by Yanagisawa, Seiga, Frasch, Wayne D.

“…The two opposed rotary molecular motors of the F0F1-ATP synthase work together to provide the majority of ATP in biological organisms. Rotation occurs in 120°…”
Get full text

Elastic coupling power stroke mechanism of the F₁-ATPase molecular motor by Martin, James L., Ishmukhametov, Robert, Spetzler, David, Hornung, Tassilo, Frasch, Wayne D.

“…The angular velocity profile of the 120° F₁-ATPase power stroke was resolved as a function of temperature from 16.3 to 44.6 °C using a ΔμATP = −31.25 k B T at…”
Get full text

F1FO ATP synthase molecular motor mechanisms by Frasch, Wayne D., Bukhari, Zain A., Yanagisawa, Seiga

“…The F-ATP synthase, consisting of F 1 and F O motors connected by a central rotor and the stators, is the enzyme responsible for synthesizing the majority of…”
Get full text

pH-dependent 11° F1FO ATP synthase sub-steps reveal insight into the FO torque generating mechanism by Yanagisawa, Seiga, Frasch, Wayne D

“…Most cellular ATP is made by rotary F 1 F O ATP synthases using proton translocation-generated clockwise torque on the F O c-ring rotor, while F 1 -ATP…”
Get full text

Anatomy of F1-ATPase powered rotation by Martin, James L., Ishmukhametov, Robert, Hornung, Tassilo, Ahmad, Zulfiqar, Frasch, Wayne D.

“…F1-ATPase, the catalytic complex of the ATP synthase, is a molecular motor that can consume ATP to drive rotation of the γ-subunit inside the ring of three…”
Get full text

Structural Asymmetry and Kinetic Limping of Single Rotary F-ATP Synthases by Sielaff, Hendrik, Yanagisawa, Seiga, Frasch, Wayne D, Junge, Wolfgang, Börsch, Michael

“…F-ATP synthases use proton flow through the F domain to synthesize ATP in the F₁ domain. In , the enzyme consists of rotor subunits γε and stator subunits…”
Get full text

Direct observation of stepped proteolipid ring rotation in E. coli FoF1-ATP synthase by Ishmukhametov, Robert, Hornung, Tassilo, Spetzler, David, Frasch, Wayne D

“…Although single‐molecule experiments have provided mechanistic insight for several molecular motors, these approaches have proved difficult for membrane bound…”
Get full text

Power Stroke Angular Velocity Profiles of Archaeal A-ATP Synthase Versus Thermophilic and Mesophilic F-ATP Synthase Molecular Motors by Sielaff, Hendrik, Martin, James, Singh, Dhirendra, Biuković, Goran, Grüber, Gerhard, Frasch, Wayne D.

“…The angular velocities of ATPase-dependent power strokes as a function of the rotational position for the A-type molecular motor A3B3DF, from the…”
Get full text

Eukaryotic yeast V1-ATPase rotary mechanism insights revealed by high-resolution single-molecule studies by Yanagisawa, Seiga, Bukhari, Zain A., Parra, Karlett J., Frasch, Wayne D.

“…Vacuolar ATP-dependent proton pumps (V-ATPases) belong to a super-family of rotary ATPases and ATP synthases. The V 1 complex consumes ATP to drive rotation of…”
Get full text

Fo-driven Rotation in the ATP Synthase Direction against the Force of F1 ATPase in the FoF1 ATP Synthase by Martin, James, Hudson, Jennifer, Hornung, Tassilo, Frasch, Wayne D.

“…Living organisms rely on the FoF1 ATP synthase to maintain the non-equilibrium chemical gradient of ATP to ADP and phosphate that provides the primary energy…”
Get full text

ΩqPCR measures telomere length from single-cells in base pair units by Xiong, Fusheng, Frasch, Wayne D

“…Abstract ΩqPCR determines absolute telomere length in kb units from single cells. Accuracy and precision of ΩqPCR were assessed using 800 bp and 1600 bp…”
Get full text

Microsecond resolution of single-molecule rotation catalyzed by molecular motors by Hornung, Tassilo, Martin, James, Spetzler, David, Ishmukhametov, Robert, Frasch, Wayne D

“…Single-molecule measurements of rotation catalyzed by the F(1)-ATPase or the F(o)F(1) ATP synthase have provided new insights into the molecular mechanisms of…”
Get more information

Elastic coupling power stroke mechanism of the F 1 -ATPase molecular motor by Martin, James L, Ishmukhametov, Robert, Spetzler, David, Hornung, Tassilo, Frasch, Wayne D

“…The angular velocity profile of the 120° F -ATPase power stroke was resolved as a function of temperature from 16.3 to 44.6 °C using a Δμ = -31.25 at a time…”
Get full text

Determination of torque generation from the power stroke of Escherichia coli F1-ATPase by Hornung, Tassilo, Ishmukhametov, Robert, Spetzler, David, Martin, James, Frasch, Wayne D.

“…The torque generated by the power stroke of Escherichia coli F1-ATPase was determined as a function of the load from measurements of the velocity of the…”
Get full text

Direct observation of stepped proteolipid ring rotation in E. coli F o F 1 -ATP synthase by Frasch, Wayne D, Ishmukhametov, Robert, Hornung, Tassilo, Spetzler, David

Get full text

Single Molecule Measurements of F1-ATPase Reveal an Interdependence between the Power Stroke and the Dwell Duration by Spetzler, David, Ishmukhametov, Robert, Hornung, Tassilo, Day, Lixia Jin, Martin, James, Frasch, Wayne D

“…Increases in the power stroke and dwell durations of single molecules of Escherichia coli F1-ATPase were measured in response to viscous loads applied to the…”
Get full text

Electron Spin Echo Envelope Modulation Spectroscopy Reveals and Distinguishes Equatorial and Axial Nitrogen Ligands Bound to VO2 by LoBrutto, Russell, Hamstra, Brent J, Colpas, Gerard J, Pecoraro, Vincent L, Frasch, Wayne D

“…ESEEM (electron spin echo envelope modulation) spectra of a series of vanadyl complexes have for the first time revealed the spectral features that arise from…”
Get full text

Padlock probe-mediated qRT-PCR for DNA computing answer determination by Xiong, Fusheng, Frasch, Wayne D.

“…Padlock probe-mediated quantitative real time PCR (PLP-qRT-PCR) was adapted to quantify the abundance of sequential 10mer DNA sequences for use in DNA…”
Get full text

The participation of metals in the mechanism of the F1-ATPase by Frasch, Wayne D

Get full text

Abundance of Escherichia coli F1-ATPase molecules observed to rotate via single-molecule microscopy with gold nanorod probes by York, Justin, Spetzler, David, Hornung, Tassilo, Ishmukhametov, Robert, Martin, James, Frasch, Wayne D

“…The abundance of E. coli F1-ATPase molecules observed to rotate using gold nanorods attached to the gamma-subunit was quantitated. Individual F1 molecules were…”
Get full text